catabolism of small carbon sources in Alkalihalobacterium alkalinitrilicum DSM 22532

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Alkalihalobacterium alkalinitrilicum DSM 22532

Pathways are sorted by completeness. Sort by name instead.

Pathway	Steps
deoxyinosine	nupA, nupB, nupC', bmpA, deoD, deoB, deoC, adh, ackA, pta
thymidine	nupC, deoA, deoB, deoC, adh, ackA, pta
sucrose	ams, fruII-ABC, 1pfk, fba, tpi
ethanol	etoh-dh-nad, adh, ackA, pta
fructose	fruII-ABC, 1pfk, fba, tpi
glycerol	glpF, glpK, glpD, tpi
L-lactate	lctP, lutA, lutB, lutC
D-lactate	lctP, D-LDH
maltose	susB, ptsG-crr
alanine	alsT
fumarate	sdcL
glucose	ptsG-crr
L-malate	sdlC
succinate	sdc
isoleucine	brnQ, bkdA, bkdB, bkdC, lpd, acdH, ech, ivdG, fadA, pccA, pccB, epi, mcm-large, mcm-small
propionate	lctP, prpE, pccA, pccB, epi, mcm-large, mcm-small
NAG	nagEIIA, nagPcb, nagA, nagB
trehalose	treEIIA, treB, treC, glk
citrate	citM, acn, icd
proline	putP, put1, putA
cellobiose	bgl, ptsG-crr
glucosamine	gamP, nagB
glutamate	dmeA, gdhA
2-oxoglutarate	Psest_0084, Psest_0085
gluconate	gntT, gntK, gnd
xylitol	fruI, x5p-reductase
valine	brnQ, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcm-large, mcm-small
leucine	brnQ, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, atoA, atoD, atoB
threonine	tdcC, ltaE, adh, ackA, pta, gcvP, gcvT, gcvH, lpd
tryptophan	tnaT, kynA, kynB, kyn, antA, antB, antC, xylE, praB, praC, praD, mhpD, mhpE, adh, ackA, pta
arginine	bgtB, artP, rocF, rocD, PRO3, put1, putA
tyrosine	aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
4-hydroxybenzoate	pcaK, pobA, praA, praB, praC, praD, mhpD, mhpE, adh, ackA, pta
deoxyribose	deoP, deoK, deoC, adh, ackA, pta
acetate	actP, ackA, pta
phenylalanine	aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
pyruvate	yjcH, actP
serine	serP, sdaB
sorbitol	SOT, sdh, scrK
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, atoA, atoD, atoB
aspartate	glt
glucose-6-P	uhpT
asparagine	ans, glt
mannose	manP, manA
glucuronate	exuT, udh, gci, garL, garR, garK
mannitol	PLT5, mt2d, scrK
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, rocD, PRO3, put1, putA
galactose	galP, galK, galT, galE, pgmA
D-alanine	cycA, dadA
ribose	rbsU, rbsK
D-serine	cycA, dsdA
xylose	xylT, xylA, xylB
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
putrescine	puuP, patA, patD, gabT, gabD
galacturonate	exuT, uxaC, uxaB, uxaA, kdgK, eda
rhamnose	rhaT, LRA1, LRA2, LRA3, LRA5, LRA6
histidine	PA5503, PA5504, PA5505, hutH, hutU, hutI, hutG
lysine	lysP, cadA, patA, patD, davT, davD, glaH, lhgD
fucose	fucP, fucU, fucI, fucK, fucA, tpi, aldA
arabinose	araE, araA, araB, araD
myoinositol	iolT, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA, tpi
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources