Alignments for a candidate for pimF in Methylocystis bryophila S285

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Methylocystis bryophila S285

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_085772004.1 B1812_RS13170 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_002117405.1:WP_085772004.1
          Length = 728

 Score =  306 bits (785), Expect = 2e-87
 Identities = 226/725 (31%), Positives = 331/725 (45%), Gaps = 60/725 (8%)

Query: 11  DQVAIVTVDSP--PVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEF 68
           D VA++T D P   +N ++  V   + + V    AD A++  V+      F  GAD++  
Sbjct: 13  DGVALLTWDMPGRSMNVITEEVMSELAQVVEKVAADAAIKGCVIASGKNAFSGGADLSML 72

Query: 69  GKP---------------------PQPPALNDVIAALENSPKPTIAAIHGTALGGGLEVA 107
            K                           L+ +   LE   KP   AIHG  LGG  E+A
Sbjct: 73  QKSRAAHGKALGEKGAEEANRLFFESARKLSQLYRRLETCGKPFAIAIHGVCLGGAFELA 132

Query: 108 LGCHFRVAVKE--AKLGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEAL 165
           L CH RV   +   K+GLPEVK+GL PGAGGTQR+ R +    A+Q +  G  +    A 
Sbjct: 133 LACHHRVLADDDKTKVGLPEVKVGLFPGAGGTQRVARIMPTGDALQFLFKGEQLSGKAAK 192

Query: 166 KHGLVEEVVENLVAGAVAFAKK-VLAEKRPLRRLRDDDSKLAAAK----ADRSIFTNAVA 220
             GL +E+     +  V+ AK  +L   + +      D K  + +        I+  A A
Sbjct: 193 AAGLAQELAPQ--SEIVSRAKDWILGGGKAIAPWDQKDFKPPSGRIFSPGGMMIWMPANA 250

Query: 221 AMTKKAR-GLEAPFACADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQ-RYAFFAER 278
              ++      A  A    +   + LP ++GL  E   F  ++ S ++ A  R  F ++ 
Sbjct: 251 IYRRETYDNYPAVKAILHCVYEGLQLPIDQGLTVEARWFAHILRSKEAAAMIRSLFLSKN 310

Query: 279 EAAKVDGVPDGTKPRPVSRVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLKRGLGIM 338
           E  K    P    P  + ++ ++GAG MG G+    A  G+ V LI+  +E   +G   +
Sbjct: 311 ELDKGARRPKDEPPTQLKKIGVLGAGFMGAGVGYVSALNGLEVVLIDRDQESADKGKATI 370

Query: 339 QKNWEATAARGGLPPDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDA 398
            K      +RG     A    +A +      E +K  DLI+EAVFE  AVK E      A
Sbjct: 371 DKLISGQVSRGKATASAKEALLARVEATPDYERLKGCDLILEAVFEDRAVKAEATKRAQA 430

Query: 399 HAKPGAVLASNTSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLT 458
              P  V ASNTS L I  +A T+ +P++ +G+HFFSP   M L EI+ G KT+  AL T
Sbjct: 431 AVGPDVVFASNTSTLPITSLAETSLKPENFIGVHFFSPVEKMLLVEIILGEKTSDKALAT 490

Query: 459 AVSIAKKIAKVPVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGP 518
           A+   + I K P+VV    GF  NR + A  ++ + +L EG  P  ++      GMP+GP
Sbjct: 491 ALDFVRMIKKTPIVVNDSRGFFANRCVLAYVREGQTMLVEGVPPAMIENAARMAGMPVGP 550

Query: 519 FAMGDLAGLDIGWR----SRKDRG------IKSEIADALCEA-GRFGQKTGKGYYKY-EQ 566
            ++ D  GLD+GW+    ++KD G       +  +   L E  GRFG+K GKG+Y Y   
Sbjct: 551 LSLNDEVGLDLGWKILQATKKDLGAAAVDPAQERVLGFLVEKNGRFGRKNGKGFYDYPAS 610

Query: 567 GSRAPMPDPEVETLINDTLAKLGLKRRD---ITDEEILERMVYPMINEGARILEEKIAAR 623
           G +   P           LA L  K  D   I  +E+  R +     E AR   E +   
Sbjct: 611 GKKTLWPG----------LAGLAEKTLDPDAIDVKELERRFLVAQAVEAARTFAEHVVTD 660

Query: 624 PSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAA 683
           P + DV  + G+G+  + GG + Y D +GLK         A A   P   P  LL  +A 
Sbjct: 661 PREADVGSILGFGFAPFTGGALSYIDGMGLKAFVALCDTLA-AKYGPRFAPPQLLRDMAR 719

Query: 684 EGKTF 688
           EG+TF
Sbjct: 720 EGETF 724


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1103
Number of extensions: 57
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 699
Length of database: 728
Length adjustment: 39
Effective length of query: 660
Effective length of database: 689
Effective search space:   454740
Effective search space used:   454740
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources