Alignments for a candidate for atoB in Methylocystis bryophila S285

GapMind for catabolism of small carbon sources

Alignments for a candidate for atoB in Methylocystis bryophila S285

Align Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 (characterized)
to candidate WP_085770214.1 B1812_RS02620 acetyl-CoA C-acetyltransferase

Query= SwissProt::P50174
         (393 letters)



>NCBI__GCF_002117405.1:WP_085770214.1
          Length = 391

 Score =  585 bits (1508), Expect = e-172
 Identities = 288/387 (74%), Positives = 332/387 (85%)

Query: 6   IVIASAARTAVGSFNGAFGNTLAHELGAAAIKAVLERAGVEAGEVDEVILGQVLPAGEGQ 65
           IVI SAARTAVGSFNGAFG   AHELGAAA+K+ LERA V   EV EVILGQVL A +GQ
Sbjct: 5   IVIVSAARTAVGSFNGAFGGVPAHELGAAAVKSALERAKVAPSEVCEVILGQVLGAAQGQ 64

Query: 66  NPARQAAMKAGLPQEKTAWGMNQLCGSGLRAVALGMQQIATGDAKVIVAGGMESMSMAPH 125
           NPARQAA+KAG+P   TA+G+NQ+CGSGLRAVAL  QQI  GDA+++VAGG ESMS++ H
Sbjct: 65  NPARQAAIKAGVPDSATAFGINQVCGSGLRAVALAAQQIQAGDAQIVVAGGQESMSLSTH 124

Query: 126 CAHLRGGVKMGDYKMIDTMIKDGLTDAFYGYHMGITAENVARKWQLTREEQDEFALASQN 185
            AH+R G KMG     DTMI DGLTDAF  YHMGITAENVA KWQ++R EQD FA+ASQN
Sbjct: 125 AAHMRAGTKMGPVSFADTMIIDGLTDAFNNYHMGITAENVAAKWQISRAEQDAFAVASQN 184

Query: 186 KAEAAQKAGRFADEIVPFVVKTRKGDVNVDQDEYIRHGATLDSIAKLRPAFDKEGTVTAG 245
           KAEAAQKAG+F DEI+P+ + TRKGDV VD DEY++HG TL+S++KLRPAF K+GTVTA 
Sbjct: 185 KAEAAQKAGKFKDEILPYTISTRKGDVVVDSDEYVKHGVTLESVSKLRPAFTKDGTVTAA 244

Query: 246 NASGLNDGAAAALLMTEAEAARRGIQPLARIVSWATAGVDPQIMGTGPIPASRKALEKAG 305
           NASGLNDGAAA ++M+ AEA +RG++PLARI +WATAGVDP IMG+GPIPA+RKALEKAG
Sbjct: 245 NASGLNDGAAALVVMSAAEAKKRGLEPLARIAAWATAGVDPSIMGSGPIPATRKALEKAG 304

Query: 306 WSVADIELVEANEAFAAQACAVNKDLGWDPSIVNVNGGAIAIGHPIGASGARVLNTLLFE 365
           W V+D+ LVEANEAFAAQA AVNKDLGWDP+IVNVNGGAIAIGHPIGASGAR+L TLL+E
Sbjct: 305 WKVSDLNLVEANEAFAAQAIAVNKDLGWDPAIVNVNGGAIAIGHPIGASGARILTTLLYE 364

Query: 366 MKRRGVSKGLATLCIGGGMGVAMCVER 392
           +KRRG  KGLATLCIGGGMG+A+ VER
Sbjct: 365 LKRRGGGKGLATLCIGGGMGIALAVER 391


Lambda     K      H
   0.317    0.132    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 502
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 391
Length adjustment: 31
Effective length of query: 362
Effective length of database: 360
Effective search space:   130320
Effective search space used:   130320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources