Alignments for a candidate for uxaA in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for uxaA in Halomonas desiderata SP1

Align Altronate dehydratase (EC 4.2.1.7) (characterized)
to candidate WP_086509098.1 BZY95_RS06185 altronate dehydratase family protein

Query= reanno::Pedo557:CA265_RS19875
         (548 letters)



>NCBI__GCF_002151265.1:WP_086509098.1
          Length = 547

 Score =  564 bits (1454), Expect = e-165
 Identities = 289/543 (53%), Positives = 363/543 (66%), Gaps = 11/543 (2%)

Query: 6   LKIHPNDNVLVALQNLAKGETVIYDGHEYILQDDIQAKHKFFMQDMNAGDHIIMYGVLVG 65
           L++H  DNV VAL++L  G  V        L + I+ KHKF + D+   D +IMYGV VG
Sbjct: 16  LRVHHADNVRVALKDLPAGHAVDDGERRLHLAEAIRHKHKFALHDLAPDDAVIMYGVTVG 75

Query: 66  KAQHFILKGGLMDTENTKHASDPYEFRPYHYEWHAPDVSKFEGRTFNGYIRSDGRVGTAN 125
           +A   I  G  + T+NT H++     +    EW APDV+ F G TF+GY R +G+VGTAN
Sbjct: 76  RATRPIPAGAAITTDNTAHSTATSVPQARRGEWQAPDVAAFAGATFDGYHRPNGQVGTAN 135

Query: 126 YWLFIPTVFCENRNLDVIREALHNELGYAVTDKYKSYAHQLVEAYKNGEILAEADPSSIG 185
            WL +P VFCENRN++V+R+ + + LG      YK  A +L+              S   
Sbjct: 136 VWLVVPLVFCENRNIEVLRQCVADALGEDPYRDYKRMARELLHG-----------SSGTA 184

Query: 186 LANPSANRVFKNVDGIKFLNHQGGCGGTRQDAAVLSKLLAAYADHPNVAGVTVLSLGCQN 245
              P   R+F NVDG++FL H  GCGGT  DA  L +LLA Y  HPNVAG TVLSLGCQ 
Sbjct: 185 AVEPPGERLFPNVDGVRFLTHTLGCGGTDDDALALCRLLAGYICHPNVAGATVLSLGCQK 244

Query: 246 LQVKDFMDDLKHRSPNFDKPLFVFEQQQSQSEEQLVKEAIRKTFIGLTEINKIERQPAPL 305
            Q++     +  R P   +P+   EQQ SQSEE L++EA+   F GL+E N+  R PAPL
Sbjct: 245 AQIEMLQTAVAERDPCGLRPVHYLEQQGSQSEEALIREALTTIFDGLSEANRTPRTPAPL 304

Query: 306 SKLVLGVKCGGSDGFSGISANPAVGYTSDLLVALGGTVLLAEFPELCGAEQQLIDRTKDE 365
           S L LGV+CGGSDGFSG+SANP VG   D LVALGG+ +L+EFPELCG E +LI R +D+
Sbjct: 305 SALTLGVECGGSDGFSGLSANPLVGAVVDRLVALGGSGILSEFPELCGVEHELIARCRDD 364

Query: 366 TAARKFIQLMTAYNQSAENVGSGFFMNPSPGNIKDGLITDAIKSTGAAKKGGTSPVEDVL 425
             A +F  LM AY + A  VG+ F MNPSPGNI+DGLITDA+KS GAAKKGG SPV DVL
Sbjct: 365 AVAERFRTLMEAYQRHAARVGADFSMNPSPGNIRDGLITDAMKSAGAAKKGGDSPVVDVL 424

Query: 426 DYTEPATKPGLNLVCTPGNDVEATTGKAASGATLILFTTGLGTPTGNPVCPTIKVSTNNA 485
           DY EP T+ GL+L+CTPGNDVE+TT  A SGA LILFTTGLGTPTGNPV P +K+S+N+ 
Sbjct: 425 DYAEPHTRAGLSLLCTPGNDVESTTALAGSGANLILFTTGLGTPTGNPVTPVLKISSNSE 484

Query: 486 LTKRMGDIIDINCGPVIEGEKTIEQMGEDILEYCIKAASGEVIPKAVLLNQDDFIPWKRG 545
           L  RMGD+ID + GP+I GE +I+++ E +L  C+  ASG  +P+AV L Q DFIPWKRG
Sbjct: 485 LAARMGDVIDFDAGPIIRGEVSIDELAERLLRLCLDTASGRYLPRAVKLAQYDFIPWKRG 544

Query: 546 VSL 548
           VSL
Sbjct: 545 VSL 547


Lambda     K      H
   0.317    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 815
Number of extensions: 33
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 548
Length of database: 547
Length adjustment: 36
Effective length of query: 512
Effective length of database: 511
Effective search space:   261632
Effective search space used:   261632
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources