Alignments for a candidate for acs in Rhodanobacter denitrificans FW104-10B01

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Rhodanobacter denitrificans FW104-10B01

Align isobutanoate/2-methylbutanoate--CoA ligase (EC 6.2.1.1) (characterized)
to candidate WP_007510062.1 LRK54_RS08400 AMP-binding protein

Query= metacyc::MONOMER-20125
         (556 letters)



>NCBI__GCF_021560695.1:WP_007510062.1
          Length = 557

 Score =  171 bits (433), Expect = 7e-47
 Identities = 170/571 (29%), Positives = 250/571 (43%), Gaps = 72/571 (12%)

Query: 9   ASSSPL---TPLGFLERAATVYGDCTSVVYDA--VSYTWSQTHRRCLCLASSIASLGIEN 63
           AS+ PL   T    ++R A  Y D  ++V  A  V  ++ Q H     +A+ + +LG+E 
Sbjct: 11  ASAQPLLGDTVGSLIDRIAVAYPDRPALVVRAQGVRLSYRQFHTEVERIAAGLLALGLEP 70

Query: 64  GHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLI-------- 115
           G  + + +PN  +   L FA P AG +L  +N       +   L+    + +        
Sbjct: 71  GERIGIWSPNRAEWVLLQFAAPKAGLVLVNINPAYRTHELEYSLNKVACRALVLPRRFKT 130

Query: 116 --FVDHLSRDLILEAIALFPKQAPVPRL------VFMADESESGNSSELGKEFFCSYKDL 167
             ++D L+ +L  E  A  P +    RL      V + DE   G            +  L
Sbjct: 131 SHYLDILT-ELAPELAAGVPGELRAARLPALREVVLLDDEPAPGTRG---------WWQL 180

Query: 168 IDRGDPDFKWVMPKSEW-----DPMILNYTSGTTSSPKGVVHCHRGI-----FIMTVDSL 217
              GD      + + E      DP+ + +TSGTT +PKG    H  I     FI     L
Sbjct: 181 AALGDAAALARLHEVERELGFDDPVNIQFTSGTTGAPKGATLTHHNIVNNGWFIGEAMRL 240

Query: 218 IDWGVPKQPVYLWTLPMFHANGWSYPWGMAAVGGTNICL----RKFDSEIIYDMIKRHGV 273
            ++     PV     P +H  G     G  A      C+      FD+    + +     
Sbjct: 241 TEYDRLCIPV-----PFYHCFGMVL--GNLACVTHGACMVIPGEGFDALATLETVAEEKC 293

Query: 274 THMCGAPVVLNMLSNAP--GSEPLKTTVQIMTAGAPPPSAVLFRT--ESLGFAVSHGYGL 329
           T + G P +       P      L T    + AG+P P  V+ R   E     ++  YG+
Sbjct: 294 TGLHGVPTMFIAELEHPRFAEFDLSTLRTGIMAGSPCPIEVMRRVVGEMHLAEITIAYGM 353

Query: 330 TETAGLVVSCAWKKEWNHLPATERARLKSRQGVGTVM-QTKIDVVDPVTGAAVKRDGSTL 388
           TET+ +         +  +P     R      VG +  Q ++ +VD   G  V R   T 
Sbjct: 354 TETSPV--------SFQTVPDDPLERRVD--SVGRIHPQLEVKLVDE-RGLIVPR--GTP 400

Query: 389 GEVVLRGGSVMLGYLKDPEGTAKSMTADGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGE 448
           GE+  RG SVMLGY +D   T + + A  W +TGD+ V+  DGY  I  R KD+II GGE
Sbjct: 401 GELCTRGYSVMLGYWEDESRTREVIDAARWMHTGDLAVIDADGYCRIVGRLKDMIIRGGE 460

Query: 449 NLSSVEVESILYSHPDILEAAVVARPDEFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSK 508
           N+   EVE  LY+HP +L+  V   PD  +GE  CA+V L++G     +E EI +YCR  
Sbjct: 461 NVYPREVEEFLYAHPKVLDVQVFGVPDTKFGEQVCAWVRLREG--AGASEAEIQDYCRHH 518

Query: 509 LPRYMVPKTVVFKEELPKTSTGKVQKFILRD 539
           L  Y VP  V F +  P T TGKVQK+ +R+
Sbjct: 519 LAYYKVPHYVRFVDAFPMTVTGKVQKYRMRE 549


Lambda     K      H
   0.319    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 766
Number of extensions: 44
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 557
Length adjustment: 36
Effective length of query: 520
Effective length of database: 521
Effective search space:   270920
Effective search space used:   270920
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources