Protein WP_092993135.1 in Thiohalomonas denitrificans HLD2

GapMind for catabolism of small carbon sources

Protein WP_092993135.1 in Thiohalomonas denitrificans HLD2

Annotation: NCBI__GCF_900102855.1:WP_092993135.1

Length: 481 amino acids

Source: GCF_900102855.1 in NCBI

Candidate for 49 steps in catabolism of small carbon sources

Pathway	Step	Score	Similar to	Id.	Cov.	Bits	Other hit	Other id.	Other bits
L-arginine catabolism	gabD	med	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized)	45%	96%	390.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
L-citrulline catabolism	gabD	med	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized)	45%	96%	390.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
putrescine catabolism	gabD	med	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized)	45%	96%	390.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
L-arginine catabolism	davD	med	glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized)	44%	99%	385.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
L-citrulline catabolism	davD	med	glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized)	44%	99%	385.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
L-lysine catabolism	davD	med	glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized)	44%	99%	385.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
L-proline catabolism	davD	med	glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized)	44%	99%	385.6	3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97	44%	381.3
4-hydroxybenzoate catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
2'-deoxyinosine catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
2-deoxy-D-ribose catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
ethanol catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-threonine catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
thymidine catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-tryptophan catabolism	adh	med	aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)	41%	90%	343.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	patD	lo	aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized)	35%	95%	287.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-citrulline catabolism	patD	lo	aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized)	35%	95%	287.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
putrescine catabolism	patD	lo	aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized)	35%	95%	287.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
4-hydroxybenzoate catabolism	praB	lo	2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized)	36%	97%	285.8	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-tryptophan catabolism	praB	lo	2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized)	36%	97%	285.8	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-fucose catabolism	aldA	lo	NAD⁺-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)	36%	98%	273.1	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-rhamnose catabolism	aldA	lo	NAD⁺-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)	36%	98%	273.1	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-threonine catabolism	aldA	lo	NAD⁺-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized)	36%	98%	273.1	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arabinose catabolism	xacF	lo	Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)	36%	97%	270.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
D-galacturonate catabolism	dopDH	lo	Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)	36%	97%	270.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
D-glucuronate catabolism	dopDH	lo	Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)	36%	97%	270.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
D-xylose catabolism	dopDH	lo	Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized)	36%	97%	270.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-phenylalanine catabolism	pad-dh	lo	phenylacetaldehyde dehydrogenase (EC 1.2.1.39) (characterized)	36%	97%	265.8	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-tryptophan catabolism	nbaE	lo	2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized)	35%	94%	259.6	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arabinose catabolism	aldA	lo	lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)	33%	98%	258.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
D-xylose catabolism	aldA	lo	lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)	33%	98%	258.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	kauB	lo	gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)	35%	95%	252.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	puuC	lo	gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)	35%	95%	252.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-citrulline catabolism	puuC	lo	gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)	35%	95%	252.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
putrescine catabolism	puuC	lo	gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized)	35%	95%	252.7	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-lysine catabolism	patD	lo	aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized)	33%	98%	246.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-valine catabolism	mmsA	lo	Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; EC 1.2.1.-; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MSDH; EC 1.2.1.27 (uncharacterized)	30%	96%	219.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	putA	lo	L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)	30%	93%	208	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	rocA	lo	L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)	30%	93%	208	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-citrulline catabolism	putA	lo	L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)	30%	93%	208	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-citrulline catabolism	rocA	lo	L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)	30%	93%	208	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-proline catabolism	putA	lo	L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)	30%	93%	208	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-isoleucine catabolism	iolA	lo	malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)	30%	95%	203.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
myo-inositol catabolism	mmsA	lo	malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)	30%	95%	203.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
propionate catabolism	iolA	lo	malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)	30%	95%	203.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-threonine catabolism	iolA	lo	malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)	30%	95%	203.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-valine catabolism	iolA	lo	malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)	30%	95%	203.4	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-lysine catabolism	amaB	lo	L-piperidine-6-carboxylate dehydrogenase; EC 1.2.1.21 (characterized, see rationale)	30%	92%	197.2	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-arginine catabolism	astD	lo	N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized)	31%	93%	167.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6
L-citrulline catabolism	astD	lo	N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized)	31%	93%	167.5	succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79)	45%	390.6

Sequence Analysis Tools

View WP_092993135.1 at NCBI

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Find functional residues: SitesBLAST

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Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Sequence

MLESPLLSHTAGYINGSWQRGVGEPFQVTNPANGEVLAEIPAMGAEETRQAIEAGRHALE
SSAGLETRRAWLQMIAEALTDNREEIGRILTHEHGKPWKEGIGEVDYAAGFFRYAGENIG
LLKPYTLQEQPRGLSWTVYHRPAGVVGLMTPWNFPIGMIAKKLSAALAADCSCVVKPSRK
TPLTMIALFDLLDRTLDLPPGKVNLVMGGACPITDTLMEHPHVRVISFTGSTAVGRQLIR
KSADGIKRLTLELGGNAPFIVFADADLDHAAEQLVSNKFRGGGQTCVCSNRIFVERPVAP
EFAEKLAEKASALRVGNGMDRVTDIGPLIDRSGYEKVRSHLTDALDKGAQMVSGGDPGEW
STARGFFRPTVIRGVAPGMLVTREETFGPLVPIAEFDSEEEVIARANDTEFGLAAYLFTA
DPERAGHVVSRLEFAHVGWNTGSGPTPEAPFGGMKQSGFGREGGREGLVEFVETQTVAQQ
P

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources