Align Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized)
to candidate WP_092487756.1 BM299_RS18040 amino acid ABC transporter permease
Query= TCDB::Q9I403 (248 letters) >NCBI__GCF_900115975.1:WP_092487756.1 Length = 234 Score = 120 bits (302), Expect = 2e-32 Identities = 76/215 (35%), Positives = 120/215 (55%), Gaps = 14/215 (6%) Query: 32 TIAIALVGWIIALALGSLLGVMRTVPNRLVSGIATAYVEIFRNVPLLVQLFIWYFLVPDL 91 T+ I ++ I LG + G+ R +++ +ATAYV+ FR PLLVQ+FI YF +P L Sbjct: 19 TLQITIISVAIGCLLGLIGGLSRLSRRKVLRFLATAYVDFFRGTPLLVQIFIVYFGIPKL 78 Query: 92 LPEGLQTWFKQDLNPTTSA-------YLSVVVCLGLFTAARVCEQVRTGIQALPYGQTSA 144 L + LQ++ T A +L+ V+ L + A V E R G+Q++ GQ A Sbjct: 79 LMD-LQSFMVDSYGMTPVADSFALPRFLAAVIACSLNSGAYVAEIFRAGVQSIERGQMEA 137 Query: 145 ARAMGFRLPQIYRHVLLPQAFRIIIPPLTSEFLNIFKNSSVASLIGLMELLAQTKQTAEF 204 AR++G Q R+V+LPQAF+ +IPPL +EF+ + K++S+ S+IG EL T+ Sbjct: 138 ARSLGMTHGQAMRYVILPQAFKRVIPPLGNEFIAMLKDTSLLSVIGFAEL---TRNGQLI 194 Query: 205 SANLFEAFTL---ATLIYFTLNMSLMLIMRLVERK 236 A +E+F + IY + +L I+ +ER+ Sbjct: 195 IARTYESFAIWLTVGFIYLIMTFTLSRIVDYLERR 229 Lambda K H 0.327 0.141 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 154 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 248 Length of database: 234 Length adjustment: 23 Effective length of query: 225 Effective length of database: 211 Effective search space: 47475 Effective search space used: 47475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory