GapMind for catabolism of small carbon sources

 

Alignments for a candidate for musK in Desulfacinum infernum DSM 9756

Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate WP_073037934.1 BUB04_RS06025 ABC transporter ATP-binding protein

Query= BRENDA::Q8NMV1
         (376 letters)



>NCBI__GCF_900129305.1:WP_073037934.1
          Length = 365

 Score =  265 bits (677), Expect = 1e-75
 Identities = 151/355 (42%), Positives = 205/355 (57%), Gaps = 27/355 (7%)

Query: 21  VKKFNLEIADGEFLVLVGPSGCGKSTTLRMLAGLENVTDGAIFIGDKDVTHVAPRDRDIA 80
           V+  +  + +G+ LV++GPSGCGK+T LR++AGLE VT G I I   DVTH+ P  R+I+
Sbjct: 18  VRGVSFSVEEGQLLVILGPSGCGKTTLLRLIAGLEPVTSGTIHIAGTDVTHLPPVKRNIS 77

Query: 81  MVFQNYALYPHMTVGENMGFALKIAGKSQDEINKRVDEAAATLGLTEFLERKPKALSGGQ 140
           MVFQ+YAL+PH+ V EN+ F LK+     DEI +R+      LGL+  L+ KP  LSGG 
Sbjct: 78  MVFQSYALFPHLNVRENIIFGLKVRKVPADEIERRLKRVVDLLGLSGRLDSKPGELSGGM 137

Query: 141 RQRVAMGRAIVRNPQVFLMDEPLSNLDAKLRVQTRTQIAALQRKLGVTTVYVTHDQTEAL 200
           +QRVA+GRAI+    V LMDEPLSNLDAKLR   R +I +LQR+LG+T +YVTHDQ EA+
Sbjct: 138 QQRVALGRAIIAEKPVTLMDEPLSNLDAKLRNSMRREICSLQRRLGITMIYVTHDQVEAM 197

Query: 201 TMGDRIAVLKDGYLQQVGAPRELYDRPANVFVAGFIGSPAMNLGTFSVKDGDATSGHARI 260
           TM DRI +++DG + Q  +P   Y+RPAN FVA FIG+P MN+       G A       
Sbjct: 198 TMADRIVLMRDGQIVQDDSPENFYERPANTFVARFIGTPPMNIVPLCPTQGGAA------ 251

Query: 261 KLSPETLAAMTPEDNGRITIGFRPEALEIIPEGESTDLSIPIKLDFVEELGSDSFLYGKL 320
            L P         D  R   G RPE L +   G+      P  +   E LGSD+F+  ++
Sbjct: 252 -LEPGGRLLFPGMDPDRYLFGIRPENLRLAESGQ------PAMVTGREYLGSDTFVSCEI 304

Query: 321 VGEGDLGSSSEDVPESGQIVVRAAPNAAPAPGSVFHARIVEGGQHNFSASTGKRL 375
            G+              +++VR         G+V H     G  + F A T +R+
Sbjct: 305 HGQ--------------EVIVRTRGRRNIREGTVVHLTWDPGDVNLFDARTQERV 345


Lambda     K      H
   0.316    0.135    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 361
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 376
Length of database: 365
Length adjustment: 30
Effective length of query: 346
Effective length of database: 335
Effective search space:   115910
Effective search space used:   115910
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory