Alignments for a candidate for cadA in Malonomonas rubra DSM 5091

GapMind for catabolism of small carbon sources

Alignments for a candidate for cadA in Malonomonas rubra DSM 5091

Align Lysine decarboxylase LdcA; EC 4.1.1.18 (characterized)
to candidate WP_072905987.1 BUB13_RS04490 aminotransferase class V-fold PLP-dependent enzyme

Query= SwissProt::Q9I2S7
         (751 letters)



>NCBI__GCF_900142125.1:WP_072905987.1
          Length = 643

 Score =  237 bits (605), Expect = 1e-66
 Identities = 185/635 (29%), Positives = 291/635 (45%), Gaps = 106/635 (16%)

Query: 78  DVVELIRVARVRAPQLPIFALGEQVTIENAPAESMADLHQLRGILYLFEDTVPFLARQVA 137
           D++  I+  R+    +PI+ LG +    N   ES+ D+       + F D +      + 
Sbjct: 89  DIISAIKAIRIT---VPIYVLGNEKQGLNIIDESI-DIES-----FFFRDDIILDPESIL 139

Query: 138 RAARN-YLAGLLPPFFRALVEHTAQSNYSWHTPGHGGGVAYRKSPVGQAFHQFFGENTLR 196
               N +      PF++A   +  ++N SWHTPGH GG+++R SP    F+ F+G N   
Sbjct: 140 GYILNDFDDRSQAPFWKAYKNYITETNDSWHTPGHCGGLSFRNSPYINDFYNFYGRNVFV 199

Query: 197 SDLSVSVPELGSLLDHTGPLAEAEDRAARNFGADHTFFVINGTSTANKIVWHSMVGREDL 256
            DLSVSV  LGSL + T  + +A++ AA  F    T+FV NG+ST+NK++  +++   + 
Sbjct: 200 GDLSVSVDSLGSLAEGTNAIGKAQEAAAYTFEVTKTYFVTNGSSTSNKVILQTLLRAGNK 259

Query: 257 VLVDRNCHKSILHSIIMTGAIPLYLTPERN-ELGIIGPIPLSEFSKQSIAAKIAASPLAR 315
           V+VDRNCHKS+ + I+ +GA P+YL+   + + GI  P  L E  +++I A         
Sbjct: 260 VIVDRNCHKSVHYGIVQSGAEPVYLSSVLHPDYGIFAPPTLEEI-REAIDA--------- 309

Query: 316 GREPKVKLAVVTNSTYDGLCYNAELIKQTLGDSVEVLHFDEAWYAYAAFHEFYDGRYGMG 375
              P  KL V+T  TY+GL  +   +          +  DEAW+AY+ FH  +  RY   
Sbjct: 310 --HPDTKLIVLTGCTYEGLLMDIRAVVDMAHAKGIKVFIDEAWFAYSLFHPTF--RYYSA 365

Query: 376 TSRSEEGPLVFATHSTHKMLAAFSQASMIHVQDGGTRKLDVARFNEAFMMHISTSPQYGI 435
                +    + THS HK+++AFSQAS IHV D      D   F+E F M++STSP+Y I
Sbjct: 366 IDSGAD----YITHSAHKVVSAFSQASYIHVND---PDFDADFFHEIFSMYVSTSPKYQI 418

Query: 436 IASLDVASAMMEGPAGRSLIQETFDEALSFRRALANVRQNLDRNDWWFGVWQPEQVEGTD 495
           IASLDV    +E   G  L+++  ++    +  + + R                      
Sbjct: 419 IASLDVCHKQLE-MEGYGLLKDLLEQVAELKEQMKSFR---------------------- 455

Query: 496 QVGTHDWVLEPSADWHGFGDIAEDYVLLDPIKVTLTTPGLSAGGKLSEQGIPAAIVSRFL 555
               H  +L+       F    +D V  DP+K+ +    L              I    L
Sbjct: 456 ----HIRILDIEDFREAFPHFKQDNVGHDPLKILIDVSRLDYSTN--------EIHRYLL 503

Query: 556 WERGLVVEKTGLYSFLVLFSMGITKGKWSTLVTELLEFKRCYDANLPLLDVLPSVAQAGG 615
            E GL +EK    + LVL ++G T+ K   L   L +  +   A++P+            
Sbjct: 504 DEIGLEIEKYTHSTMLVLLTLGGTRSKVIRLYNALKKLDQ-KKASIPI------------ 550

Query: 616 KRYNGVGLRDLSDAMHASYRDNATAKAMKRMYTVLPEVAMRPSEAYDKLVRGEVEAVPIA 675
                                   +K  K+    LP++ +  + A  K   GE E++   
Sbjct: 551 ------------------------SKNRKKKLEGLPKITLTCTPA--KAFYGERESLSYE 584

Query: 676 RLEGRIAAVMLVPYPPGIPLIMPGERFTEATRSIL 710
              GR +A ++ PYPPGIPL++PG+   E   + L
Sbjct: 585 CAIGRTSAGLVTPYPPGIPLLLPGQAVEEGAIAYL 619


Lambda     K      H
   0.320    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1031
Number of extensions: 43
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 751
Length of database: 643
Length adjustment: 39
Effective length of query: 712
Effective length of database: 604
Effective search space:   430048
Effective search space used:   430048
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources