GapMind for catabolism of small carbon sources

 

Alignments for a candidate for msiK in Paucidesulfovibrio gracilis DSM 16080

Align MsiK protein, component of The cellobiose/cellotriose (and possibly higher cellooligosaccharides), CebEFGMsiK [MsiK functions to energize several ABC transporters including those for maltose/maltotriose and trehalose] (characterized)
to candidate WP_078717009.1 B5D49_RS06865 spermidine/putrescine ABC transporter ATP-binding protein PotA

Query= TCDB::P96483
         (377 letters)



>NCBI__GCF_900167125.1:WP_078717009.1
          Length = 370

 Score =  226 bits (576), Expect = 8e-64
 Identities = 109/226 (48%), Positives = 157/226 (69%)

Query: 28  IEDGEFLVLVGPSGCGKSTSLRMLAGLEDVNGGAIRIGDRDVTHLPPKDRDIAMVFQNYA 87
           I  GEFL ++GPSGCGK+T LR+LAG E  + G I +  R +  +PP  R +  VFQ+YA
Sbjct: 30  IRHGEFLTILGPSGCGKTTLLRLLAGFESPSSGEIILDGRSMRDVPPDGRRVNTVFQSYA 89

Query: 88  LYPHMTVADNMGFALKIAGVPKAEIRQKVEEAAKILDLTQYLDRKPKALSGGQRQRVAMG 147
           L+PHM+V DN+ F L+++G+PK EI ++V +A +++ L     R+P +LSGGQ+QRVA+ 
Sbjct: 90  LFPHMSVFDNVAFGLRMSGIPKVEIGERVAKALRMVGLAGQAGRRPTSLSGGQQQRVAIA 149

Query: 148 RAIVREPQVFLMDEPLSNLDAKLRVSTRTQIASLQRRLGITTVYVTHDQVEAMTMGDRVA 207
           RA+V  P V L+DEPLS LD KLRV  RT++  L+R +GIT ++VTHDQ EA +M DRV 
Sbjct: 150 RAVVNRPLVLLLDEPLSALDYKLRVQMRTELKQLRREMGITFIFVTHDQEEAFSMSDRVV 209

Query: 208 VLKDGLLQQVDSPRNMYDKPANLFVAGFIGSPAMNLVEVPITDGGV 253
           V+ +G + QV +P  +Y++P N+FVA F+G   +       +DGG+
Sbjct: 210 VMNEGCVAQVGTPVEVYEQPVNMFVARFVGETNVFEGVAGQSDGGI 255


Lambda     K      H
   0.317    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 374
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 370
Length adjustment: 30
Effective length of query: 347
Effective length of database: 340
Effective search space:   117980
Effective search space used:   117980
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory