Alignments for a candidate for vorA in Paucidesulfovibrio gracilis DSM 16080

GapMind for catabolism of small carbon sources

Alignments for a candidate for vorA in Paucidesulfovibrio gracilis DSM 16080

Align Ketoisovalerate oxidoreductase subunit VorA; VOR; 2-oxoisovalerate oxidoreductase alpha chain; 2-oxoisovalerate-ferredoxin oxidoreductase subunit alpha; EC 1.-.-.- (characterized)
to candidate WP_078717838.1 B5D49_RS11420 thiamine pyrophosphate-dependent enzyme

Query= SwissProt::P80907
         (478 letters)



>NCBI__GCF_900167125.1:WP_078717838.1
          Length = 256

 Score =  253 bits (647), Expect = 4e-72
 Identities = 123/244 (50%), Positives = 160/244 (65%)

Query: 15  VFERKGGSAPTATHYCAGCGHGILHKLIGEAIDELGIQERSVMISPVGCAVFAYYYFDCG 74
           VF+R        THYC GC HG+ H+L+GE +DE+ ++E S++I  +GC+VF Y Y D  
Sbjct: 9   VFDRPESVIDRPTHYCPGCHHGVAHRLVGELLDEMKLREESILIGSIGCSVFLYNYLDID 68

Query: 75  NVQVAHGRAPAVGTGISRAEDTPVVLLYQGDGDLASIGLNETIQAANRGEKMAVFFVNNT 134
            V+  HGRAPAVGTG  RA     VL YQGDGDLASIG+ E + AANRGE++ V FVNNT
Sbjct: 69  AVEAPHGRAPAVGTGAKRARPDKFVLTYQGDGDLASIGMAEIMHAANRGERICVVFVNNT 128

Query: 135 VYGMTGGQMAPTTLIGEVTVTCPGGRDPRYAGYPLHMCELLDNLQAPVFIERVSLADPKS 194
           VYGMTGGQMAPTTL+G+ T TCPGGR     G P+ M E++  L    F  R SL   K+
Sbjct: 129 VYGMTGGQMAPTTLVGQRTTTCPGGRCRDKEGLPIKMTEIISQLGGVAFAGRASLDSVKN 188

Query: 195 IRKAKRAVKRALEIQRDGKGYAFVEVLSPCPTNLRQDAEGAERFLKEEMEREFPVKNFRD 254
           IR AK+ +++A E Q   +G+ FVE+LS CPTN +     A   ++EEM   FP+  ++D
Sbjct: 189 IRTAKKYLRKAFECQTQDRGFGFVELLSACPTNWKMPPVQANERIREEMIPYFPLGVYKD 248

Query: 255 RSSE 258
            + E
Sbjct: 249 VTEE 252


Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 364
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 478
Length of database: 256
Length adjustment: 29
Effective length of query: 449
Effective length of database: 227
Effective search space:   101923
Effective search space used:   101923
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align Ketoisovalerate oxidoreductase subunit VorA; VOR; 2-oxoisovalerate oxidoreductase alpha chain; 2-oxoisovalerate-ferredoxin oxidoreductase subunit alpha; EC 1.-.-.- (characterized)
to candidate WP_078717839.1 B5D49_RS11425 2-oxoacid:acceptor oxidoreductase family protein

Query= SwissProt::P80907
         (478 letters)



>NCBI__GCF_900167125.1:WP_078717839.1
          Length = 181

 Score =  117 bits (292), Expect = 4e-31
 Identities = 63/171 (36%), Positives = 107/171 (62%), Gaps = 6/171 (3%)

Query: 301 IAGFGGQGVLSMGLTLAQAACSEGRHTSWYPAYGPEQRGGTSSCGVVISGERVGSPAVDT 360
           IAGFGGQGV+ +G  LA +  ++G + ++ P YGPE RGGT++C VV+S E +GSP +  
Sbjct: 9   IAGFGGQGVMLIGNLLAYSGMNQGLNVTYIPVYGPEMRGGTANCTVVLSDEDIGSPIIHR 68

Query: 361 PDVLVAFNQPSLDEFAGDVREGGIVLYDTA--TADFSKKENLRAIGVPALEIAKEHGTGR 418
           P  L+  N+PSLD+F   + +GG+ + +++   A+ ++ + ++++ VPA +IA E G  R
Sbjct: 69  PKSLIIMNRPSLDKFQPRLEDGGVQIINSSLVDAELAETDRVKSVFVPANDIADEIGNTR 128

Query: 419 AANTAMLGV-MMALGITGLDEESFRDAIRFTFSGK-DKIIDINLKILEAGA 467
            AN   +G  + A G+  +  ++  D++    S    K+I +N K ++AGA
Sbjct: 129 MANMVAIGAFLQATGVIPI--QAAIDSLENVISAHYKKLIPMNAKAIQAGA 177


Lambda     K      H
   0.318    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 194
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 478
Length of database: 181
Length adjustment: 26
Effective length of query: 452
Effective length of database: 155
Effective search space:    70060
Effective search space used:    70060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources