Alignments for a candidate for xad in Paucidesulfovibrio gracilis DSM 16080

GapMind for catabolism of small carbon sources

Alignments for a candidate for xad in Paucidesulfovibrio gracilis DSM 16080

Align Xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate WP_078718407.1 B5D49_RS14320 dihydroxy-acid dehydratase

Query= reanno::pseudo6_N2E2:Pf6N2E2_1668
         (594 letters)



>NCBI__GCF_900167125.1:WP_078718407.1
          Length = 554

 Score =  289 bits (739), Expect = 2e-82
 Identities = 186/550 (33%), Positives = 288/550 (52%), Gaps = 29/550 (5%)

Query: 35  GMTREELQSGRPIIGIAQTGSDLTPCNRHHLELAQRVKAGIRDAGGIPMEFPV----HPI 90
           G+TREE+   RP++G+    +++ P + H   +A  VK G+R +GG PMEFP       +
Sbjct: 23  GITREEMD--RPLVGVCNAFNEVIPGHVHLRTIADAVKNGVRMSGGTPMEFPAIGVCDGL 80

Query: 91  AEQSRRPTAALDRNLAYLGLVEILH-GYPLDGVVLTTGCDKTTPACLMAAATTDLPAIVL 149
           A        +L         +EI+   +P D +V  T CDK  P  LMA    ++PA+++
Sbjct: 81  AMNHEGMRFSLPSREIIADSIEIMATAHPFDALVCVTNCDKIVPGMLMAMLRLNIPAVIV 140

Query: 150 SGGPMLDGHHKGELIGSGTVLWHARNLMAAGEIDYEGFMEMTTAASPSVGHCNTMGTALS 209
           SGGPML G  K ++    TV +     +  G++      E+   A P+ G C+ M TA +
Sbjct: 141 SGGPMLAGKQKKQVSDLITV-FEGVGRVKRGDMSETELAELEECACPTCGSCSGMFTANT 199

Query: 210 MNALAEALGMSLPGCASIPAPYRERGQMAYATGKRICDLVRQDIRPSQIMTRQAFENAIA 269
           MN L+EA+G++LPG  +IPA    R ++A   G  + +L+ Q I P  I+T  +  NA+A
Sbjct: 200 MNCLSEAIGLALPGNGTIPATAAARVRLAKQAGMAVMNLLEQGITPRDIVTTDSVRNAVA 259

Query: 270 VASALGASSNCPPHLIAIARHMGVELSLEDWQRIGEDVPLLVNCMPAGKYLGEGFHRAGG 329
           +  ALG S+N   HL A+     +ELSLE +  I   VP +    PAG Y  E    AGG
Sbjct: 260 MDMALGGSTNTVLHLPAVFHEAELELSLELFDEISRQVPNICKLSPAGHYHIEDLDAAGG 319

Query: 330 VPSVMHELQKAGRLHEDCATVSGKTIGEIVS--NSLTSNTDVIHPFDTPLKHRAGFIVLS 387
           +P+VM  +   G LH DC TV+GKT+G+ +   N+   + DVI P D     + G  +L 
Sbjct: 320 IPAVMRRVADTGALHLDCLTVTGKTVGQNLDDLNADVLDPDVIRPLDKAYSPQGGIAILR 379

Query: 388 GNFFD-SAIMKMSVVG-EAFRKTYLSEPGAENSFEARAIVFEGPEDYHARIDDPALDIDE 445
           GN  +   ++K S V  E  ++T             +A VF+  ED    I    +   +
Sbjct: 380 GNLAEQGCVVKQSAVAPEMMQRT------------GKARVFDSEEDAVEAILGNGIKAGD 427

Query: 446 RCILVIRGVGTVGYPGSAEVVNMAPPAALIKQGI-DSLPCLGDGRQSGTSASPSILNMSP 504
             ++VIR  G  G PG  E+  + P +A+   G+ +S+  + DGR SG +   +I ++SP
Sbjct: 428 --VVVIRYEGPKGGPGMREM--LTPTSAIAGMGLGESVALITDGRFSGGTRGAAIGHVSP 483

Query: 505 EAAVGGGLALLKTNDRLKVDLNTRTVNLLIDDAEMAQRRREWIPNIPPSQTPWQELYRQL 564
           EAA GG + L++  D +++D+  R + LL+D+ E+AQRR+ W P      +P+   Y   
Sbjct: 484 EAAEGGLIGLVREGDSIRIDIPERRIELLVDETELAQRRQNWTPLQKEVTSPFLRRYAAR 543

Query: 565 VGQLSTGGCL 574
           V   +TGG +
Sbjct: 544 VTSAATGGVM 553


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 952
Number of extensions: 42
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 594
Length of database: 554
Length adjustment: 36
Effective length of query: 558
Effective length of database: 518
Effective search space:   289044
Effective search space used:   289044
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources