Alignments for a candidate for mglA in Tistlia consotensis USBA 355

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Tistlia consotensis USBA 355

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate WP_085126169.1 B9O00_RS28465 multiple monosaccharide ABC transporter ATP-binding protein

Query= TCDB::O05176
         (512 letters)



>NCBI__GCF_900177295.1:WP_085126169.1
          Length = 509

 Score =  686 bits (1769), Expect = 0.0
 Identities = 352/506 (69%), Positives = 411/506 (81%)

Query: 5   ILEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTYEGEI 64
           ILEMR+ITKTFPGV AL+NVNL+V++GEIHAL GENGAGKSTLMK+LSGVYP G Y+G+I
Sbjct: 4   ILEMRDITKTFPGVVALDNVNLQVRQGEIHALAGENGAGKSTLMKILSGVYPYGDYQGDI 63

Query: 65  HYEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASNGVISWQQTFNRT 124
            YEG V+ FR I DSE  GI+IIHQELALVP LSIAENIFLGNE+A  GVI W  T    
Sbjct: 64  VYEGEVKKFRGIADSEAEGIVIIHQELALVPELSIAENIFLGNEIARFGVIDWHATNELA 123

Query: 125 RELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSEALLN 184
            + LK VGLKESP T + D+G+GKQQLVEIAKALSK VKLLILDEPT+SLNE DS ALL 
Sbjct: 124 ADHLKWVGLKESPRTKVQDLGLGKQQLVEIAKALSKEVKLLILDEPTSSLNEQDSAALLK 183

Query: 185 LLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRNMVGR 244
           LL EFR +G++SI+I+HKLNE+ +VAD ITVLRDG TV TLDC    ISED IIR+MVGR
Sbjct: 184 LLAEFRERGISSILISHKLNEISQVADSITVLRDGTTVSTLDCAAASISEDDIIRDMVGR 243

Query: 245 DLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMGAGRT 304
            L DR+PPR   IGE +LEV++W+  H QH DR+V+ D + TVR+GEVVG+AGLMGAGRT
Sbjct: 244 SLSDRFPPRHPKIGELLLEVEHWSVRHPQHLDRRVVKDASFTVRRGEVVGLAGLMGAGRT 303

Query: 305 EFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLNDNILHN 364
           E AMS+FG+SYG  I G+V I GK VDVSTV +AI AGL Y TEDRK  GLVL+D + HN
Sbjct: 304 ELAMSLFGRSYGADIAGEVRIRGKAVDVSTVERAIAAGLCYATEDRKSYGLVLDDIVKHN 363

Query: 365 TTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVLSKWLF 424
            TLANL GV+   +ID  +E++VA D+R RL I++S I Q+T+NLSGGNQQKVVL KWLF
Sbjct: 364 LTLANLEGVANGPVIDHAREIRVAEDYRRRLNIKTSSIAQKTLNLSGGNQQKVVLGKWLF 423

Query: 425 SNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIYVMNEG 484
           ++P+VLILDEPTRGIDVGAKYEIY+II  LAA GKG+L+ISSEMPELLG CDRIYVMN G
Sbjct: 424 ADPEVLILDEPTRGIDVGAKYEIYSIITDLAAAGKGILVISSEMPELLGICDRIYVMNAG 483

Query: 485 RIVAELPKGEASQESIMRAIMRSGEK 510
           R+V ELP  EASQE IM  I+R+ EK
Sbjct: 484 RLVGELPAAEASQEKIMSMIIRTAEK 509


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 767
Number of extensions: 28
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 512
Length of database: 509
Length adjustment: 35
Effective length of query: 477
Effective length of database: 474
Effective search space:   226098
Effective search space used:   226098
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources