Alignments for a candidate for iatA in Tistlia consotensis USBA 355

GapMind for catabolism of small carbon sources

Alignments for a candidate for iatA in Tistlia consotensis USBA 355

Align Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized)
to candidate WP_085124759.1 B9O00_RS21320 sugar ABC transporter ATP-binding protein

Query= TCDB::B8H229
         (515 letters)



>NCBI__GCF_900177295.1:WP_085124759.1
          Length = 518

 Score =  361 bits (927), Expect = e-104
 Identities = 205/496 (41%), Positives = 302/496 (60%), Gaps = 8/496 (1%)

Query: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTF 62
           LL++  VS+ FPGV ALD+VD  +  GEVH L GENGAGKST+I +++ A     G   F
Sbjct: 23  LLELRGVSRRFPGVLALDKVDFQLRPGEVHVLFGENGAGKSTMISMIAGALRPSEGEFRF 82

Query: 63  AGQVLDPRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLRADAQ 122
            G+ ++       R  LGI+ ++QEF+L P+++V EN++LG E  RLGL+D   L   A 
Sbjct: 83  RGEPVELNSVHHARG-LGISAVFQEFSLVPQMTVEENLFLGAESTRLGLLDRRALHDRAD 141

Query: 123 ALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAI 182
           A+L  LG PL P   V  LT AEQQMVEIAKA      ++I+DEPTA+L+ RE  +L  +
Sbjct: 142 AILKRLGFPLRPRDRVLHLTRAEQQMVEIAKAFRSELSVLILDEPTASLTERETAQLFKL 201

Query: 183 IAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRLMVGRHVE 242
           I  +KA+ V VIY++HRL E++ + DR TV+RDGR+VA+ D       ++VRLM GR  +
Sbjct: 202 IEQVKAQGVGVIYITHRLSEIRRIGDRITVLRDGRYVATVDAQTTPEGELVRLMTGRVYD 261

Query: 243 FERRKRRRPPGAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLA 302
               K R  PG  +L VE +T      +A G + + S +   GEIVGLAGLVG+G++++A
Sbjct: 262 ELFPKVRFKPGRELLSVERLT------TASGSVVEASLSVCAGEIVGLAGLVGSGKSEVA 315

Query: 303 RLIFGADPIAAGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLSLPS 362
           R  FG + IA G V +  + +  + P+  ++ G   VP DR+ +G  +   +R N+SLPS
Sbjct: 316 RACFGLEKIAEGHVRLKGEEVTGQGPKRMLKRGFFYVPPDRRDEGLMMMRPVRENVSLPS 375

Query: 363 LK-ALSALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTP 421
           L  A    G  +D R E D V    ++L ++    E A+   SGGNQQKVLL ++     
Sbjct: 376 LTLAPFGNGALLDRRGETDRVRRLAEQLNLQPPRIERAVEAFSGGNQQKVLLAKSRTRPV 435

Query: 422 KVLIVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIV 481
           +V   DEPT G+D+G +  +++ + DL + G  +++ISS+L EV+ +S+R  VF  G + 
Sbjct: 436 QVFAFDEPTVGVDVGTRVAIYEFIRDLCEAGAGILLISSDLPEVLNLSNRAYVFYRGRVQ 495

Query: 482 ADLDAQTATEEGLMAY 497
           A+L     TEE ++++
Sbjct: 496 AELAGDEITEEKVLSH 511



 Score = 89.7 bits (221), Expect = 2e-22
 Identities = 77/257 (29%), Positives = 121/257 (47%), Gaps = 25/257 (9%)

Query: 252 PGAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFGADPI 311
           P A +L++ GV+   P + A   L +V F  R GE+  L G  GAG++ +  +I GA   
Sbjct: 19  PAAPLLELRGVSRRFPGVLA---LDKVDFQLRPGEVHVLFGENGAGKSTMISMIAGALRP 75

Query: 312 AAGRVLVDDKPLRLRSPRDAIQAGIM-------LVPEDRKQQGCFLDHSIRRNLSLPSLK 364
           + G      +P+ L S   A   GI        LVP+   ++  FL     R        
Sbjct: 76  SEGEFRFRGEPVELNSVHHARGLGISAVFQEFSLVPQMTVEENLFLGAESTR-------- 127

Query: 365 ALSALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTPKVL 424
               LG  +D RA  D  +   ++L   +   +  +  L+   QQ V + +A      VL
Sbjct: 128 ----LGL-LDRRALHDRADAILKRLGFPLRPRDRVL-HLTRAEQQMVEIAKAFRSELSVL 181

Query: 425 IVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVADL 484
           I+DEPT  +     A++ +++  +   GV V+ I+  L+E+  + DRI V R+G  VA +
Sbjct: 182 ILDEPTASLTERETAQLFKLIEQVKAQGVGVIYITHRLSEIRRIGDRITVLRDGRYVATV 241

Query: 485 DAQTATEEGLMAYMATG 501
           DAQT T EG +  + TG
Sbjct: 242 DAQT-TPEGELVRLMTG 257


Lambda     K      H
   0.320    0.136    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 628
Number of extensions: 34
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 518
Length adjustment: 35
Effective length of query: 480
Effective length of database: 483
Effective search space:   231840
Effective search space used:   231840
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources