Alignments for a candidate for mglA in Tistlia consotensis USBA 355

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Tistlia consotensis USBA 355

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_085124750.1 B9O00_RS21270 sugar ABC transporter ATP-binding protein

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_900177295.1:WP_085124750.1
          Length = 500

 Score =  364 bits (935), Expect = e-105
 Identities = 206/496 (41%), Positives = 315/496 (63%), Gaps = 9/496 (1%)

Query: 3   PILEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEII 62
           P +E+  I K F GV AL  V    +PGEVHA++GENGAGKSTL+KI+ G    D GE+ 
Sbjct: 7   PRVELVGISKAFAGVPALDRVDFTLHPGEVHALLGENGAGKSTLIKIMTGALAADAGEMR 66

Query: 63  YEGRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIFIDYKKMYREAE 122
            +GR V     +EA   GI TV+QE++++  LSVA N+ +G E +R   I ++    +A 
Sbjct: 67  LDGRAVAVGSTAEARALGISTVYQEVNLVPTLSVARNVTLGREPRRFGLISWRAAREKAR 126

Query: 123 KFMKEEFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLFE 182
             +K    +EID E  LG + +A+QQ+V IARA+ + ++VL+LDEPT+SL  +ET  LF 
Sbjct: 127 ARLKR-LSLEIDVERALGSFPVAVQQLVAIARALEEDSRVLVLDEPTASLDAQETRLLFR 185

Query: 183 VVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMVGRKL 242
           +++ LK +G+A++FISH L++++EI D++SVLR+G  +G+ +   L + +++ +M+GR+L
Sbjct: 186 ILRDLKAQGMAVVFISHFLDQVYEISDRISVLRNGRLVGSAATAALPQRELITLMLGREL 245

Query: 243 EKFYIKEAHEP---GEVVLEVKNLSGERF-ENVSFSLRRGEILGFAGLVGAGRTELMETI 298
           E+   + A  P   G  VL  + L   R+ E     LR GE +G AGL+G+GRTE  + I
Sbjct: 246 ERVEQRAAAAPKAAGAPVLAAEKLGRRRWIEPFDLVLRAGEAIGLAGLLGSGRTETAKLI 305

Query: 299 FGFRPKRGGEIYIEGKRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIMHNVSLPSLDR 358
           FG      G + ++G  +    P  A+ +GI   PEDRK  GL+L +S+  N+ L    +
Sbjct: 306 FGAVRADSGRLTLDGAPLGHASPRRALRRGIAFCPEDRKAEGLVLDLSVRENILLAL--Q 363

Query: 359 IKKGPFISFKREKE--LADWAIKTFDIRPAYPDRKVLYLSGGNQQKVVLAKWLALKPKIL 416
            K+G      R+++  LA+  I+   I  +  ++ V  LSGGNQQKVVLA+ LA KP++L
Sbjct: 364 TKRGWLRPLPRDEQRRLAEAMIEALGIATSDAEKPVGQLSGGNQQKVVLARALASKPRVL 423

Query: 417 ILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRIAVMSFGKLAGII 476
           +LDEPTRGIDVGA AEI  ++ +L  EG+ +++ SSEL E++ +SDR+AV+   ++ G I
Sbjct: 424 VLDEPTRGIDVGAHAEIVALVRRLCAEGLALLVASSELDEIVAVSDRVAVLRDRRIVGEI 483

Query: 477 DAKEASQEKVMKLAAG 492
              E ++E +++L AG
Sbjct: 484 AGAEITRETIIELIAG 499



 Score = 90.9 bits (224), Expect = 9e-23
 Identities = 64/226 (28%), Positives = 114/226 (50%), Gaps = 6/226 (2%)

Query: 269 ENVSFSLRRGEILGFAGLVGAGRTELMETIFGFRPKRGGEIYIEGKRVEINHPLDAIEQG 328
           + V F+L  GE+    G  GAG++ L++ + G      GE+ ++G+ V +    +A   G
Sbjct: 25  DRVDFTLHPGEVHALLGENGAGKSTLIKIMTGALAADAGEMRLDGRAVAVGSTAEARALG 84

Query: 329 IGLVPEDRKKLGLILIMSIMHNVSLPSLDRIKKGPFISFKREKELADWAIKTFDIRPAYP 388
           I  V ++   + L+  +S+  NV+L    R  +   IS++  +E A   +K   +     
Sbjct: 85  ISTVYQE---VNLVPTLSVARNVTLGREPR--RFGLISWRAAREKARARLKRLSLEIDV- 138

Query: 389 DRKVLYLSGGNQQKVVLAKWLALKPKILILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVI 448
           +R +       QQ V +A+ L    ++L+LDEPT  +D      ++RI+  L  +G+ V+
Sbjct: 139 ERALGSFPVAVQQLVAIARALEEDSRVLVLDEPTASLDAQETRLLFRILRDLKAQGMAVV 198

Query: 449 MISSELPEVLQMSDRIAVMSFGKLAGIIDAKEASQEKVMKLAAGLE 494
            IS  L +V ++SDRI+V+  G+L G        Q +++ L  G E
Sbjct: 199 FISHFLDQVYEISDRISVLRNGRLVGSAATAALPQRELITLMLGRE 244


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 594
Number of extensions: 24
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 494
Length of database: 500
Length adjustment: 34
Effective length of query: 460
Effective length of database: 466
Effective search space:   214360
Effective search space used:   214360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources