GapMind for catabolism of small carbon sources

 

L-proline catabolism in Halococcus hamelinensis 100A6

Best path

ectP, put1, putA

Rules

Overview: Proline degradation in GapMind is based on MetaCyc pathway I via glutamate semialdehyde dehydrogenase (link) and pathway II via 5-aminopentanoate (link). (MetaCyc describes 5-aminopentanoate, also known as 5-aminovalerate, as a fermentative end product, but it is further degraded

53 steps (29 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
ectP proline transporter EctP C447_RS13495 C447_RS15070
put1 proline dehydrogenase C447_RS03770
putA L-glutamate 5-semialdeyde dehydrogenase C447_RS01765 C447_RS14150
Alternative steps:
aapJ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), substrate-binding component AapJ
aapM ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM)
aapP ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP C447_RS05920 C447_RS15655
aapQ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ)
AAT20.2 proline transporter
atoB acetyl-CoA C-acetyltransferase C447_RS07970 C447_RS02785
AZOBR_RS08235 proline ABC transporter, permease component 1 C447_RS09440 C447_RS08150
AZOBR_RS08240 proline ABC transporter, permease component 2 C447_RS09445
AZOBR_RS08245 proline ABC transporter, ATPase component 1 C447_RS09450 C447_RS08140
AZOBR_RS08250 proline ABC transporter, ATPase component 2 C447_RS09455 C447_RS08135
AZOBR_RS08260 proline ABC transporter, substrate-binding component
BAC2 basic amino acid carrier BAC2
betS proline transporter BetS C447_RS15070 C447_RS13495
CCNA_00435 proline transporter
davD glutarate semialdehyde dehydrogenase C447_RS12725 C447_RS14150
davT 5-aminovalerate aminotransferase C447_RS12035 C447_RS11500
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase C447_RS08105 C447_RS15595
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase C447_RS15595 C447_RS15300
gcdG succinyl-CoA:glutarate CoA-transferase C447_RS07395 C447_RS12490
gcdH glutaryl-CoA dehydrogenase C447_RS03890 C447_RS02805
glaH glutarate 2-hydroxylase, succinate-releasing (GlaH or CsiD)
HSERO_RS00870 proline ABC transporter, substrate-binding component
HSERO_RS00885 proline ABC transporter, permease component 1 C447_RS09440
HSERO_RS00890 proline ABC transporter, permease component 2 C447_RS09445
HSERO_RS00895 proline ABC transporter, ATPase component 1 C447_RS09450 C447_RS08140
HSERO_RS00900 proline ABC transporter, ATPase component 2 C447_RS09455 C447_RS08135
hutV proline ABC transporter, ATPase component HutV C447_RS15655 C447_RS12675
hutW proline ABC transporter, permease component HutW C447_RS15660
hutX proline ABC transporter, substrate-binding component HutX
lhgD L-2-hydroxyglutarate dehydrogenase or oxidase (LhgD or LhgO)
N515DRAFT_2924 proline transporter
natA proline ABC transporter, ATPase component 1 (NatA) C447_RS09450 C447_RS08140
natB proline ABC transporter, substrate-binding component NatB C447_RS09460
natC proline ABC transporter, permease component 1 (NatC)
natD proline ABC transporter, permease component 2 (NatD) C447_RS09440
natE proline ABC transporter, ATPase component 2 (NatE) C447_RS09455 C447_RS08135
opuBA proline ABC transporter, ATPase component OpuBA/BusAA C447_RS15655 C447_RS11075
opuBB proline ABC transporter, fused permease and substrate-binding components OpuBB/BusAB
prdA D-proline reductase, prdA component
prdB D-proline reductase, prdB component
prdC D-proline reductase, electron transfer component PrdC
prdF proline racemase
proP proline:H+ symporter ProP
PROT1 proline transporter
proV proline ABC transporter, ATPase component ProV C447_RS15655 C447_RS07635
proW proline ABC transporter, permease component ProW C447_RS15660
proX proline ABC transporter, substrate-binding component ProX
proY proline:H+ symporter
putP proline:Na+ symporter
SLC6A7 proline:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory