Alignments for a candidate for carA in Shewanella sp. ANA-3

GapMind for Amino acid biosynthesis

Alignments for a candidate for carA in Shewanella sp. ANA-3

Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate 7023737 Shewana3_0967 carbamoyl phosphate synthase small subunit (RefSeq)

Query= SwissProt::P0A6F1
         (382 letters)



>FitnessBrowser__ANA3:7023737
          Length = 386

 Score =  605 bits (1560), Expect = e-178
 Identities = 295/382 (77%), Positives = 331/382 (86%)

Query: 1   MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI 60
           + KSALLVLEDGT F G AIGA G +VGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI
Sbjct: 5   LTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI 64

Query: 61  GNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTR 120
           GN GTN+ D ES+ VHA GL+IRDLPL+AS+FRN + LS YLK +N+V IADIDTRKLTR
Sbjct: 65  GNTGTNNEDVESNGVHACGLIIRDLPLVASSFRNQQTLSDYLKANNVVGIADIDTRKLTR 124

Query: 121 LLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGG 180
           +LREKGAQ GCI+AG + D A AL  A+AFPGL GMDLAKEVTT +AY W +GSW L GG
Sbjct: 125 ILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTDKAYPWRKGSWRLVGG 184

Query: 181 LPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSN 240
           LPE    + L + VVAYD+G K+NILRMLVDRGC +T+VPA+TSA DVL MNPDG+FLSN
Sbjct: 185 LPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGVFLSN 244

Query: 241 GPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDV 300
           GPGDP PCDYAI AIQ+ L+TD P+FGICLGHQLLALASGAKT+KMKFGHHG NHPV ++
Sbjct: 245 GPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNI 304

Query: 301 EKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGP 360
           EK  VMIT+QNHGFA DEATLPAN++VTHKSLFDG+LQGIH TDKPAFSFQGHPEASPGP
Sbjct: 305 EKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGP 364

Query: 361 HDAAPLFDHFIELIEQYRKTAK 382
           +DAAPLFDHFIELIEQYR+ AK
Sbjct: 365 NDAAPLFDHFIELIEQYRQNAK 386


Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 551
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 386
Length adjustment: 30
Effective length of query: 352
Effective length of database: 356
Effective search space:   125312
Effective search space used:   125312
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate 7023737 Shewana3_0967 (carbamoyl phosphate synthase small subunit (RefSeq))
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01368.hmm
# target sequence database:        /tmp/gapView.28663.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01368  [M=361]
Accession:   TIGR01368
Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   6.2e-152  491.6   0.0   7.1e-152  491.5   0.0    1.0  1  lcl|FitnessBrowser__ANA3:7023737  Shewana3_0967 carbamoyl phosphat


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__ANA3:7023737  Shewana3_0967 carbamoyl phosphate synthase small subunit (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  491.5   0.0  7.1e-152  7.1e-152       1     360 [.       9     379 ..       9     380 .. 0.96

  Alignments for each domain:
  == domain 1  score: 491.5 bits;  conditional E-value: 7.1e-152
                         TIGR01368   1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaeskkikvkglv 77 
                                       a lvledGtvf+g+++ga++  vGevvFnTsmtGYqEiltDpsY++qiv+ltyp+ign+g+n+ed+es+ +++ gl+
  lcl|FitnessBrowser__ANA3:7023737   9 ALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDVESNGVHACGLI 85 
                                       579************************************************************************** PP

                         TIGR01368  78 vkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavistek.sekeelvekakespkvkevnl 153
                                       +++l   +s++r++++L+++lk +++v+i+++DTR+l++ lRekg+++++i++ +  ++ ++ ++ak+ p +k+++l
  lcl|FitnessBrowser__ANA3:7023737  86 IRDLPLVASSFRNQQTLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGAdLDEAKALAAAKAFPGLKGMDL 162
                                       ***************************************************987615677888999*********** PP

                         TIGR01368 154 vkevstkeayeleq...........kakkegkklrvvvidlGvKenilreLvkrgvevtvvpadtsaeeikklnpdg 219
                                       +kev+t++ay +++           ++ +e  k++vv++d+GvK+nilr+Lv+rg++vtvvpa+tsa+++ ++npdg
  lcl|FitnessBrowser__ANA3:7023737 163 AKEVTTDKAYPWRKgswrlvgglpeDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDG 239
                                       *************9877776665533556666699****************************************** PP

                         TIGR01368 220 illsnGPGdPaaveeaietvkklleakiPifGIclGhqllalalgaktyklkfGhrGaNhpvkdlktgrveitsqNH 296
                                       ++lsnGPGdP+ +++aie ++++l++++PifGIclGhqllala+gakt+k+kfGh+GaNhpv ++++g+v+itsqNH
  lcl|FitnessBrowser__ANA3:7023737 240 VFLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNH 316
                                       ***************************************************************************** PP

                         TIGR01368 297 gyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQyHPeaspGphdteylFdefvelik 360
                                       g+a de++l+++ ++vth++l+Dg+++g++ +++p+fs Q HPeaspGp+d++ lFd+f+eli+
  lcl|FitnessBrowser__ANA3:7023737 317 GFAADEATLPAN-IKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIE 379
                                       ********8866.************************************************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (361 nodes)
Target sequences:                          1  (386 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 10.52
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis