Align 3-isopropylmalate dehydratase subunit LeuD (EC 4.2.1.33) (characterized)
to candidate 7026625 Shewana3_3758 isopropylmalate isomerase small subunit (RefSeq)
Query= ecocyc::LEUD-MONOMER (201 letters) >FitnessBrowser__ANA3:7026625 Length = 201 Score = 240 bits (613), Expect = 1e-68 Identities = 118/198 (59%), Positives = 144/198 (72%), Gaps = 1/198 (0%) Query: 3 EKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPDFV 62 + F HTGL V +D+AN+DTD IIPKQFL KVTR GFG HLF+DWR+LD+ G+ PNPDF Sbjct: 2 QPFTSHTGLAVMIDSANIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGEVPNPDFT 61 Query: 63 LNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLLPV 122 LN P+Y GASILLA+ENFGCGSSREHAPWAL D+G + +IAPSFADIFYGNS NN LLPV Sbjct: 62 LNKPRYSGASILLAQENFGCGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLPV 121 Query: 123 KLSDAEVDELFALVKANPGIHFDVDLEAQEVKAG-EKTYRFTIDAFRRHCMMNGLDSIGL 181 KLS EV +L V + G VDL +V + + FT+ RH ++NGLD+IGL Sbjct: 122 KLSANEVRQLMDEVASEEGAQITVDLTTCKVTSPLGAEFSFTLAESARHKLLNGLDAIGL 181 Query: 182 TLQHDDAIAAYEAKQPAF 199 TL H I YE++ P++ Sbjct: 182 TLSHGIQIGEYESQIPSW 199 Lambda K H 0.322 0.138 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 161 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 201 Length of database: 201 Length adjustment: 21 Effective length of query: 180 Effective length of database: 180 Effective search space: 32400 Effective search space used: 32400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 45 (21.9 bits)
Align candidate 7026625 Shewana3_3758 (isopropylmalate isomerase small subunit (RefSeq))
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00171.hmm # target sequence database: /tmp/gapView.17544.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00171 [M=188] Accession: TIGR00171 Description: leuD: 3-isopropylmalate dehydratase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-85 272.2 0.0 1.3e-85 272.0 0.0 1.0 1 lcl|FitnessBrowser__ANA3:7026625 Shewana3_3758 isopropylmalate is Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__ANA3:7026625 Shewana3_3758 isopropylmalate isomerase small subunit (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 272.0 0.0 1.3e-85 1.3e-85 1 185 [. 1 186 [. 1 189 [. 0.98 Alignments for each domain: == domain 1 score: 272.0 bits; conditional E-value: 1.3e-85 TIGR00171 1 mkefkkltGlvvpldkanvdtdaiipkqflkkikrtGfgkhlfyewryldekGkepnpefvlnvpqyqgasillare 77 m++f ++tGl+v +d an+dtd+iipkqfl k++r Gfg hlf++wryld+ G+ pnp+f ln p+y gasilla+e lcl|FitnessBrowser__ANA3:7026625 1 MQPFTSHTGLAVMIDSANIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGEVPNPDFTLNKPRYSGASILLAQE 77 89*************************************************************************** PP TIGR00171 78 nfGcGssrehapwalkdyGfkviiapsfadifynnsfkngllpirlseeeveellalvk.nkglkltvdleaqkvkd 153 nfGcGssrehapwal+d+G++ iiapsfadify+ns++ngllp++ls +ev++l+ v+ ++g ++tvdl + kv++ lcl|FitnessBrowser__ANA3:7026625 78 NFGCGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLPVKLSANEVRQLMDEVAsEEGAQITVDLTTCKVTS 154 **********************************************************9899*************** PP TIGR00171 154 segkvysfeidefrkhcllnGldeigltlqke 185 g sf++ e +h llnGld igltl + lcl|FitnessBrowser__ANA3:7026625 155 PLGAEFSFTLAESARHKLLNGLDAIGLTLSHG 186 *****************************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (188 nodes) Target sequences: 1 (201 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 5.50 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory