GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapL in Shewanella sp. ANA-3

Align N-acetyldiaminopimelate deacetylase; EC 3.5.1.47 (uncharacterized)
to candidate 7026812 Shewana3_3941 carboxypeptidase (RefSeq)

Query= curated2:B1YJ90
         (370 letters)



>lcl|FitnessBrowser__ANA3:7026812 Shewana3_3941 carboxypeptidase
           (RefSeq)
          Length = 470

 Score =  183 bits (465), Expect = 7e-51
 Identities = 127/381 (33%), Positives = 186/381 (48%), Gaps = 31/381 (8%)

Query: 5   IEMRRELHKIPEPGFKEFKTQAFILDQIRSYPEDRVSYDTFETGVFVRVKG--LTGNRT- 61
           I+ RR+LH+ PE   +EF+T   I   ++S   + V      TGV   +KG  L G +  
Sbjct: 73  IDWRRDLHQHPELSNREFRTSKIIEKHLKSLGLE-VQTGIAHTGVVAILKGGKLKGGKPG 131

Query: 62  --IGYRADIDGLPIEEATGLPFCSEHP--------GFMHACGHDVHASIALGLLRRIVEL 111
             I  RAD+D LP+ E   +PF S+          G MHACGHD H ++ +G+   +V++
Sbjct: 132 PLIAIRADMDALPVTEVVDVPFASKATDTYRGKTVGVMHACGHDTHVAMLMGVAENLVKV 191

Query: 112 P--VMDDVVFLFQPAEEGP-----GGAEPMIKSPLFEKYRPSEMYGLHVAPEYPVGTIAS 164
              +  DV+F+FQPAEEG      GGAE M+K  LF K +P +++G+HV    P G I  
Sbjct: 192 KDSLAGDVMFIFQPAEEGAPDGEEGGAELMLKQGLFAKRKPDQVFGMHVTSSMPSGMIGV 251

Query: 165 RPGVLFASAREVHITIYGQSGHAAFPHLTIDTVVAQAALIMQLQTIVSRSINPMNC-SVI 223
           R G   AS     I + G+  H + P   +D +VA A +I  +QTIVSR ++     +V+
Sbjct: 252 RSGPAMASGDSFTIKVKGRQTHGSRPWNGVDPIVAAAQIITNVQTIVSRQVDITKAPAVV 311

Query: 224 TIGKVDAGIRENVIAGRALLDGTMRALNGTDMEKLEQRVRDIIRGIEASFGVKIDLQFGN 283
           + G V+ GIR N+I     L GT+R  +      ++ R+ +I      + G     +   
Sbjct: 312 SFGAVNGGIRSNIIPDEVELIGTIRTFDQPMRADIKVRLAEIAELSAKTLGATATTEIHQ 371

Query: 284 RYYEVVNDQRVVDKFS---SFVKMNANYIECDAAMTGEDFGFMLKEIPGMMFWLGVN--- 337
            Y  VVN+  +V       + V  +   IE       EDF F   E PGM F+LGV    
Sbjct: 372 GYPVVVNNPELVASMRPVLASVVGDKMLIEPGLITGAEDFSFYALESPGMFFFLGVTPKG 431

Query: 338 ---NATSGLHQPTLNPDEEAI 355
                 +  H P    DE A+
Sbjct: 432 TDPETAASNHSPAFYVDESAL 452


Lambda     K      H
   0.323    0.140    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 421
Number of extensions: 24
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 470
Length adjustment: 31
Effective length of query: 339
Effective length of database: 439
Effective search space:   148821
Effective search space used:   148821
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory