Aligments for a candidate for aroE in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Aligments for a candidate for aroE in Burkholderia phytofirmans PsJN

Align shikimate dehydrogenase (EC 1.1.1.25) (characterized)
to candidate BPHYT_RS27375 BPHYT_RS27375 shikimate 5-dehydrogenase

Query= BRENDA::Q88IJ7
         (272 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS27375 BPHYT_RS27375 shikimate
           5-dehydrogenase
          Length = 277

 Score =  352 bits (904), Expect = e-102
 Identities = 187/277 (67%), Positives = 217/277 (78%), Gaps = 5/277 (1%)

Query: 1   MSDRYAVIGRPINHTKSPLIHGLFAQASNQQLEYGAIEGSLDDFEA---QVLQFRSEGGK 57
           M+D+Y+VIG PI HTKSPLIHGLFAQ + Q + Y AIEG L   EA    V  FR+ GGK
Sbjct: 1   MTDQYSVIGNPIGHTKSPLIHGLFAQETQQDMSYTAIEGPLSPHEAFAETVNAFRAAGGK 60

Query: 58  GMNITAPFKLRAFELADRRSERAQLARAANALKFEDGRIVAENFDGIGLLRDIEENLGEP 117
           GMN+TAPFKL+AF +AD RSERAQLA AANALKFEDGRI AENFDG+GL+RDIE NL  P
Sbjct: 61  GMNVTAPFKLKAFAMADERSERAQLAGAANALKFEDGRIAAENFDGVGLVRDIEVNLDLP 120

Query: 118 LRNRRVLLLGAGGAVRGALLPFLQAGPSELVIANRDMAKALALRNELD-HSRLRISRYEA 176
           +  +RVL+LGAGGA+RGALLPFL A P ELVIANRD+AKA AL  ++   + L    YE 
Sbjct: 121 MAGKRVLVLGAGGAMRGALLPFLAARPVELVIANRDVAKAEALVAQIGARASLIACGYED 180

Query: 177 LEGQS-FDIVVNATSASLTADLPPLPADVLGEAALAYELAYGKGLTPFLRLAREQGQARL 235
           L   S FD+VVNATSASLT +LPP+P +V      AYELAYGKGLTPFLRLAR  G   +
Sbjct: 181 LAAMSGFDLVVNATSASLTGELPPVPPNVFNPKGAAYELAYGKGLTPFLRLARNAGVLGI 240

Query: 236 ADGVGMLVEQAAEAFAWWRGVRPDTRAVINQLTIPLE 272
           ADGVGMLVEQAAEAFAWWRG+RP TR++I+QLT+PL+
Sbjct: 241 ADGVGMLVEQAAEAFAWWRGIRPRTRSLIDQLTVPLD 277


Lambda     K      H
   0.320    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 239
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 272
Length of database: 277
Length adjustment: 25
Effective length of query: 247
Effective length of database: 252
Effective search space:    62244
Effective search space used:    62244
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate BPHYT_RS27375 BPHYT_RS27375 (shikimate 5-dehydrogenase)
to HMM TIGR00507 (aroE: shikimate dehydrogenase (EC 1.1.1.25))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00507.hmm
# target sequence database:        /tmp/gapView.2522.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00507  [M=270]
Accession:   TIGR00507
Description: aroE: shikimate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                -----------
    7.7e-77  244.0   0.0    9.2e-77  243.8   0.0    1.0  1  lcl|FitnessBrowser__BFirm:BPHYT_RS27375  BPHYT_RS27375 shikimate 5-dehydr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__BFirm:BPHYT_RS27375  BPHYT_RS27375 shikimate 5-dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  243.8   0.0   9.2e-77   9.2e-77       2     265 ..       4     272 ..       3     276 .. 0.96

  Alignments for each domain:
  == domain 1  score: 243.8 bits;  conditional E-value: 9.2e-77
                                TIGR00507   2 llgviGnpikhSksplihnaalkqlgleleYlafevei...eelekalsgikalglkGvnvTvPfKeevl 68 
                                               ++viGnpi h ksplih  +++++++++ Y+a+e ++   e++ +++++++a+g kG+nvT PfK +++
  lcl|FitnessBrowser__BFirm:BPHYT_RS27375   4 QYSVIGNPIGHTKSPLIHGLFAQETQQDMSYTAIEGPLsphEAFAETVNAFRAAGGKGMNVTAPFKLKAF 73 
                                              589******************************99765111569************************** PP

                                TIGR00507  69 ellDeieesakligavNTlkledgklvgynTDgiGlvssLek.lsklksekrvliiGAGGaakavaleLl 137
                                              +++De +e+a+l+ga N lk edg++ ++n Dg+Glv ++e  l+ + ++krvl++GAGGa ++++l++l
  lcl|FitnessBrowser__BFirm:BPHYT_RS27375  74 AMADERSERAQLAGAANALKFEDGRIAAENFDGVGLVRDIEVnLDLPMAGKRVLVLGAGGAMRGALLPFL 143
                                              *****************************************99*************************99 PP

                                TIGR00507 138 ka.dkeviiaNRtvekaeelaerlqelgeilalsleevelkk.vdliinatsaglsgeideaevkaellk 205
                                               a + e++iaNR v+kae+l+ ++ +  + +a   e++   + +dl++natsa+l+ge+  ++v++++++
  lcl|FitnessBrowser__BFirm:BPHYT_RS27375 144 AArPVELVIANRDVAKAEALVAQIGARASLIACGYEDLAAMSgFDLVVNATSASLTGEL--PPVPPNVFN 211
                                              9879*********************************999888****************..********* PP

                                TIGR00507 206 egklvvDlvynpletpllkeakkkg.tkvidGlgMlvaQaalsFelwtgvepdvekvfeal 265
                                               + +++ l+y ++ tp+l++a++ g   ++dG+gMlv+Qaa +F  w+g+ p   + +++l
  lcl|FitnessBrowser__BFirm:BPHYT_RS27375 212 PKGAAYELAYGKGLTPFLRLARNAGvLGIADGVGMLVEQAAEAFAWWRGIRPRTRSLIDQL 272
                                              *************************99*************************998888776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (277 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.21
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis