Align glutamyl-tRNAGln amidotransferase subunit B (EC 6.3.5.7) (characterized)
to candidate BPHYT_RS01560 BPHYT_RS01560 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Query= metacyc::MONOMER-13956 (476 letters) >FitnessBrowser__BFirm:BPHYT_RS01560 Length = 491 Score = 442 bits (1136), Expect = e-128 Identities = 229/486 (47%), Positives = 329/486 (67%), Gaps = 15/486 (3%) Query: 3 FETVIGLEVHVELKTKSKIFSSSPTPFGAEANTQTSVIDLGYPGVLPVLNKEAVEFAMKA 62 +E VIGLE H +L T+SKIFS + T FGA NTQ S +DL PG LPV+N+ AVE A++ Sbjct: 5 WEVVIGLETHAQLSTQSKIFSGTATQFGAAPNTQASPVDLALPGTLPVMNRGAVERAIQF 64 Query: 63 AMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKR------- 115 +A+ +A + F RKNYFYPD PK YQISQ++ P+ + G + I+V K Sbjct: 65 GLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKAGKEAYEK 124 Query: 116 -IGITRLHLEEDAGKLTHTG-DGYSLVDFNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKS 173 + +TR HLEEDAGK H G + +D NR GTPL+EIV+EP++R+ EA AY + L + Sbjct: 125 VVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKTLHT 184 Query: 174 IIQYTGVSDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQE 233 ++ + G+ D M+EGS RCDAN+S+RP+GQ EFGT+ E+KNLNSF F+++ +++E +RQ Sbjct: 185 LVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEVRRQI 244 Query: 234 QVLLSGFFIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYIDDEWKERVKASI 293 +++ G + QETR YD ++T MR KE + DYRYFP+PDL+ L ID W ERVKA + Sbjct: 245 ELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWIERVKAEM 304 Query: 294 PELPDERRKRYIEELGFAAYDAMVLTLTKEMADFFEETVQK--GAEAKQASNWLMGEVSA 351 ELP ++R++ + G YDA VLT +K MA ++E V K A AK A+NWLMGEVS+ Sbjct: 305 TELPVAIQQRFVSQYGLTPYDANVLTSSKAMAAYYEAVVSKVGPANAKVAANWLMGEVSS 364 Query: 352 YLNAEQKELADVALTPEGLAGMIKLIEKGTISSKIAKKVFKELIEKGGD----AEKIVKE 407 LN E ++A+ ++ LA +++ I GTIS+KIAK++F + E+ A++I++ Sbjct: 365 QLNREGLDIAENPVSSAQLALLLQRIADGTISNKIAKEIFLAIWEEKATDESAADRIIEA 424 Query: 408 KGLVQISDEGVLLKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNK 467 KGL QISD G L ++ E L NP+S+E+F+ GK++A L+GQ MKA+KG+ANP VN+ Sbjct: 425 KGLKQISDTGALEAIIDEVLAANPKSVEEFRAGKEKAFNALIGQAMKATKGKANPAQVNE 484 Query: 468 ILLEEI 473 +L +++ Sbjct: 485 LLKKKL 490 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 561 Number of extensions: 13 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 476 Length of database: 491 Length adjustment: 34 Effective length of query: 442 Effective length of database: 457 Effective search space: 201994 Effective search space used: 201994 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 52 (24.6 bits)
Align candidate BPHYT_RS01560 BPHYT_RS01560 (aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.27548.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-174 566.6 0.0 2.3e-174 566.4 0.0 1.0 1 lcl|FitnessBrowser__BFirm:BPHYT_RS01560 BPHYT_RS01560 aspartyl/glutamyl- Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__BFirm:BPHYT_RS01560 BPHYT_RS01560 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 566.4 0.0 2.3e-174 2.3e-174 3 480 .. 4 490 .. 2 491 .] 0.98 Alignments for each domain: == domain 1 score: 566.4 bits; conditional E-value: 2.3e-174 TIGR00133 3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnski 72 ++e+viGlE+H ql t+sK+F+ + +++ pNt+ +pv+l+lPG+lPv+N+ av+ A++ +la+++ lcl|FitnessBrowser__BFirm:BPHYT_RS01560 4 QWEVVIGLETHAQLSTQSKIFSGTATQFGA-APNTQASPVDLALPGTLPVMNRGAVERAIQFGLAIGAT- 71 689*************************99.9************************************9. PP TIGR00133 73 vsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke........keigierlhlEeDtgksq 134 v++ s+F+RK+YfYpDlPkgyqi+q+++P++++G+++i++ ++e k ++++r hlEeD+gks lcl|FitnessBrowser__BFirm:BPHYT_RS01560 72 VAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEkagkeayeKVVNLTRAHLEEDAGKSL 141 678************************************9998889999**9****************** PP TIGR00133 135 ykesdkdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsi 204 ++ + ++ +D+NR+g+PLlEiVt+P+++sa ea+a+ k l++++ +l+i dg+++eGs+R+D+Nvs+ lcl|FitnessBrowser__BFirm:BPHYT_RS01560 142 HED--FAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKTLHTLVTWLGICDGNMQEGSFRCDANVSV 209 *94..4789************************************************************* PP TIGR00133 205 rlkGqekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDY 274 r++Gq ++gtr+EiKNlns++ +e+ai+yE+ Rq++l+++g +v+qetr +d k t s+R+Ke+++DY lcl|FitnessBrowser__BFirm:BPHYT_RS01560 210 RPVGQAEFGTRAEIKNLNSFRFLEEAIQYEVRRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDY 279 ********************************************************************** PP TIGR00133 275 RYfpePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklike 344 RYfp+Pdl+p++id ++++ v+++++elP a ++r++++ygl +da+vl+s + ++ ++e vv+ +++ lcl|FitnessBrowser__BFirm:BPHYT_RS01560 280 RYFPDPDLMPLVIDAAWIER-VKAEMTELPVAIQQRFVSQYGLTPYDANVLTSSKAMAAYYEAVVSKVGP 348 *******************9.*********************************************9988 PP TIGR00133 345 p..klavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellen....kkdp 408 + k a+nW++ e+ ++Ln++ +++ae+ +++ +la l++ i +g+is+k+ake++ + e+ ++ + lcl|FitnessBrowser__BFirm:BPHYT_RS01560 349 AnaKVAANWLMGEVSSQLNREGLDIAENPVSSAQLALLLQRIADGTISNKIAKEIFLAIWEEkatdESAA 418 76799***************************************************99999977777889 PP TIGR00133 409 kklieklgliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllke 478 +++ie++gl qisd+ +l +i++ev++ npk+ve++++gkeka++ l+Gq+mk tkg+a+p++v++llk+ lcl|FitnessBrowser__BFirm:BPHYT_RS01560 419 DRIIEAKGLKQISDTGALEAIIDEVLAANPKSVEEFRAGKEKAFNALIGQAMKATKGKANPAQVNELLKK 488 9********************************************************************* PP TIGR00133 479 ll 480 +l lcl|FitnessBrowser__BFirm:BPHYT_RS01560 489 KL 490 98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (491 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 9.55 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory