Alignments for a candidate for thrB in Burkholderia phytofirmans PsJN

GapMind for Amino acid biosynthesis

Alignments for a candidate for thrB in Burkholderia phytofirmans PsJN

Align Homoserine kinase; HK; HSK; EC 2.7.1.39 (characterized)
to candidate BPHYT_RS33645 BPHYT_RS33645 homoserine kinase

Query= SwissProt::P29364
         (316 letters)



>FitnessBrowser__BFirm:BPHYT_RS33645
          Length = 331

 Score =  222 bits (565), Expect = 1e-62
 Identities = 133/318 (41%), Positives = 174/318 (54%), Gaps = 14/318 (4%)

Query: 1   MSVFTPLERSTLEAFLAPYDLGRLRDFRGIAEGSENSNFFVSLEHGEFVLTLVERGPVQD 60
           M+VFT +    L  +++ YDLG + +FRGI+ G ENSNFF++   GE+VLT+ E+   + 
Sbjct: 1   MAVFTAVTEPQLAEWMSHYDLGDVVEFRGISSGIENSNFFLTTTRGEYVLTIFEKLTAEQ 60

Query: 61  LPFFIELLDVLHEDGLPVPYALRTRDGEALRRLEGKPALLQPRLAGRHERQPNAHHCQEV 120
           LPF+++L+  L    +PVP  +   DG     L GKPA +  +L G  E  P   HC EV
Sbjct: 61  LPFYLDLMRHLAAHRVPVPDPMPREDGALFGTLHGKPAAIVTKLEGAPELAPGVEHCIEV 120

Query: 121 GDLLGHLHAATRGRILERPSDRGLPWMLEQGANLAPRLPEQARALLAPALAEIAAL--DA 178
           G +L  +H A R     +P+ R LPW  E    + P L  + R LL+  LA   A    A
Sbjct: 121 GQMLARMHLAGRDYTGYQPNLRSLPWWRETVPTILPFLQGEQRELLSSELAHQEAFFASA 180

Query: 179 ERPALPRANLHADLFRDNVLF-----DGPH---LAGLIDFYNA-CSGWMLYDLAITLNDW 229
           +  ALP    HADLFRDN +F     D  H   L G  DFY A C  W L+D+A+T+NDW
Sbjct: 181 DYAALPEGPCHADLFRDNAMFAHAAPDTGHEVRLGGFFDFYFAGCDKW-LFDVAVTVNDW 239

Query: 230 CSN-TDGSLDPARARALLAAYANRRPFTALEAEHWPSMLRVACVRFWLSRLIAAEAFAGQ 288
           C +   G LD AR  A+L AY   RPFTA E  HW  MLR    RFW+SRL         
Sbjct: 240 CVDLATGKLDNARTEAMLRAYQTVRPFTAEENRHWGDMLRAGAYRFWVSRLYDFYLPRAA 299

Query: 289 DVL-IHDPAEFEIRLAQR 305
           ++L  HDP  FE  L +R
Sbjct: 300 ELLKPHDPGHFERILRER 317


Lambda     K      H
   0.324    0.140    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 299
Number of extensions: 9
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 316
Length of database: 331
Length adjustment: 28
Effective length of query: 288
Effective length of database: 303
Effective search space:    87264
Effective search space used:    87264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 48 (23.1 bits)

Align candidate BPHYT_RS33645 BPHYT_RS33645 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00938.hmm
# target sequence database:        /tmp/gapView.19408.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00938  [M=307]
Accession:   TIGR00938
Description: thrB_alt: homoserine kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                -----------
   5.9e-100  320.5   0.0   6.8e-100  320.3   0.0    1.0  1  lcl|FitnessBrowser__BFirm:BPHYT_RS33645  BPHYT_RS33645 homoserine kinase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__BFirm:BPHYT_RS33645  BPHYT_RS33645 homoserine kinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  320.3   0.0  6.8e-100  6.8e-100       1     306 [.       1     315 [.       1     316 [. 0.95

  Alignments for each domain:
  == domain 1  score: 320.3 bits;  conditional E-value: 6.8e-100
                                TIGR00938   1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflellt 70 
                                              mav+t v++ +l ++++ ydlG++++++Gi++G+ensn++ltt++g yvLt++ek + ae+LPf+l+l++
  lcl|FitnessBrowser__BFirm:BPHYT_RS33645   1 MAVFTAVTEPQLAEWMSHYDLGDVVEFRGISSGIENSNFFLTTTRGEYVLTIFEKLT-AEQLPFYLDLMR 69 
                                              9*******************************************************9.************ PP

                                TIGR00938  71 hLaerglpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkeerk 140
                                              hLa++ +pv+ p++++dG++ ++L+GkPaa+v  L+G     P +e+c evg++la++hlag d+++ + 
  lcl|FitnessBrowser__BFirm:BPHYT_RS33645  70 HLAAHRVPVPDPMPREDGALFGTLHGKPAAIVTKLEGAPELAPGVEHCIEVGQMLARMHLAGRDYTGYQP 139
                                              ********************************************************************** PP

                                TIGR00938 141 ndlrleaWsilaakkfkvleqleeelaalldkeldalkkflpr....dLPrgvihadlfkdnvll..... 201
                                              n lr   W+ ++  +  +l++l+ e+ +ll++el++ + f++      LP+g  hadlf+dn ++     
  lcl|FitnessBrowser__BFirm:BPHYT_RS33645 140 N-LRSLPWWRETVPT--ILPFLQGEQRELLSSELAHQEAFFASadyaALPEGPCHADLFRDNAMFahaap 206
                                              *.***********99..******************9998876422227****************944444 PP

                                TIGR00938 202 d.gdk..lkgvidfyfaCedallydlaiavndWcfe.addkldaaaakallkgyeavrpLseeekaafpv 267
                                              d g++  l+g+ dfyfa  d +l+d+a++vndWc++ a +kld+a++ a+l +y++vrp+++ee+  +  
  lcl|FitnessBrowser__BFirm:BPHYT_RS33645 207 DtGHEvrLGGFFDFYFAGCDKWLFDVAVTVNDWCVDlATGKLDNARTEAMLRAYQTVRPFTAEENRHWGD 276
                                              223445589**************************96789****************************** PP

                                TIGR00938 268 llrgaalrfllsrlldlvftqagelvvakdPaeferkLk 306
                                              +lr++a rf++srl d++ ++a el ++ dP +fer+L+
  lcl|FitnessBrowser__BFirm:BPHYT_RS33645 277 MLRAGAYRFWVSRLYDFYLPRAAELLKPHDPGHFERILR 315
                                              *************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (307 nodes)
Target sequences:                          1  (331 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 11.68
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis