Align dihydroxyacid dehydratase (EC 4.2.1.9) (characterized)
to candidate BPHYT_RS04815 BPHYT_RS04815 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-18815 (557 letters) >FitnessBrowser__BFirm:BPHYT_RS04815 Length = 557 Score = 989 bits (2557), Expect = 0.0 Identities = 481/556 (86%), Positives = 528/556 (94%) Query: 1 MAFNKRSQNITQGVARSPNRSMYYALGYKKEDFDKPMVGIANGHSTITPCNAGLQRLADA 60 MA+N+RS+NITQGVARSPNRSMYYALGY+K DFDKPM+GIANGHSTITPCNAGLQRLADA Sbjct: 1 MAYNRRSKNITQGVARSPNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADA 60 Query: 61 AIDAIKASDANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAAQGQWMDGVVVI 120 A+ AIK +DANPQ+FGTPTISDGMSMGTEGMKYSL+SREVI+DCIET QGQWMDGVVVI Sbjct: 61 AVAAIKGADANPQIFGTPTISDGMSMGTEGMKYSLVSREVISDCIETCVQGQWMDGVVVI 120 Query: 121 GGCDKNMPGGMIALARTNVPGIYVYGGTIKPGNWKGKDLTIVSSFEAVGEFTAGRMSQED 180 GGCDKNMPGGMI + RTNVP IYVYGGTI+PGNWKG DLTIVSSFEAVGEFTAGRMSQED Sbjct: 121 GGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWKGTDLTIVSSFEAVGEFTAGRMSQED 180 Query: 181 FEGVEKNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240 F+G+E+NACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV Sbjct: 181 FDGIEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV 240 Query: 241 EAIKQDIKPRDIITRKSIENAVALIMATGGSTNAVLHYLAIAHAAEVEWTIDDFERIRRK 300 E++K+D+KPRDI+T+KSIENAVA+IMATGGSTNAVLH+LAIAHAAEVEW+IDDFER+R+K Sbjct: 241 ESVKKDLKPRDIVTKKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIDDFERMRKK 300 Query: 301 VPVICNLKPSGQYVATDLHKAGGIPQVMKILLKAGMLHGDCLTITGRTLAEELENVPDTP 360 VPVICNLKPSGQ+VATDLHKAGGIPQVMKILL AG+LHGDC+TITGRT+AEEL++VP P Sbjct: 301 VPVICNLKPSGQFVATDLHKAGGIPQVMKILLDAGLLHGDCITITGRTIAEELKDVPGKP 360 Query: 361 RADQDVILPIEKALYAEGHLAILKGNLAEEGAVAKITGLKNPVITGPARVFEDEQSAMEA 420 RADQ VI PI++ALY EGHLAILKGNLA +GAVAKITGLKNPVITGPARVF+DEQSA+EA Sbjct: 361 RADQQVIFPIDQALYKEGHLAILKGNLAVDGAVAKITGLKNPVITGPARVFDDEQSALEA 420 Query: 421 ILADKINAGDILVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV 480 ILADKI AGD++VLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVG ITDGRFSGGTWGMV Sbjct: 421 ILADKIVAGDVVVLRYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMV 480 Query: 481 VGHVAPEAYVGGTIALVQEGDSITIDAHKLLLQLNVADEELARRRANWKQPAPRYTRGVL 540 VGHVAPEA+VGGTIA VQEGDSITIDAHKLLLQLN+ + EL RRRA W+QP PRYTRGV+ Sbjct: 481 VGHVAPEAFVGGTIAFVQEGDSITIDAHKLLLQLNIDNAELERRRAAWQQPKPRYTRGVM 540 Query: 541 AKFSKLASTASKGAVT 556 AK+ LA A+KGA+T Sbjct: 541 AKYFALAQPANKGAIT 556 Lambda K H 0.316 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1169 Number of extensions: 43 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 557 Length of database: 557 Length adjustment: 36 Effective length of query: 521 Effective length of database: 521 Effective search space: 271441 Effective search space used: 271441 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate BPHYT_RS04815 BPHYT_RS04815 (dihydroxy-acid dehydratase)
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.2174.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9e-225 733.2 3.7 1e-224 733.0 3.7 1.0 1 lcl|FitnessBrowser__BFirm:BPHYT_RS04815 BPHYT_RS04815 dihydroxy-acid deh Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__BFirm:BPHYT_RS04815 BPHYT_RS04815 dihydroxy-acid dehydratase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 733.0 3.7 1e-224 1e-224 1 541 [. 18 556 .. 18 557 .] 0.99 Alignments for each domain: == domain 1 score: 733.0 bits; conditional E-value: 1e-224 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamg 70 ++r++++a+G++++d++kP+i+++n++++i+P++ l+ la+++ ++i+ a + ++ f+t ++sDG++mg lcl|FitnessBrowser__BFirm:BPHYT_RS04815 18 PNRSMYYALGYEKADFDKPMIGIANGHSTITPCNAGLQRLADAAVAAIKGADANPQIFGTPTISDGMSMG 87 68******************************************************************** PP TIGR00110 71 heGmkysLpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktkls 140 +eGmkysL+sre+i+D +et v+++++D++vvi+ CDk++PG ++++lr+n+P+i v+GG++ +g+ k + lcl|FitnessBrowser__BFirm:BPHYT_RS04815 88 TEGMKYSLVSREVISDCIETCVQGQWMDGVVVIGGCDKNMPGGMIGMLRTNVPSIYVYGGTIRPGNWK-G 156 ********************************************************************.9 PP TIGR00110 141 ekidlvdvfeavgeyaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsa 210 ++++v+ feavge +ag++s+e+ + ie++acP++gsC+G++tan+m++ +ealG+sl +sst++ ++ lcl|FitnessBrowser__BFirm:BPHYT_RS04815 157 TDLTIVSSFEAVGEFTAGRMSQEDFDGIEQNACPSTGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQ 226 ********************************************************************** PP TIGR00110 211 ekkelakksgkrivelvkknikPrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdr 280 ek ++a +s++ +ve vkk++kPrdi+tk+++ena+++++a+GGstn+vLh laia+ a+v+ s+ddf+r lcl|FitnessBrowser__BFirm:BPHYT_RS04815 227 EKVDSAAESARVLVESVKKDLKPRDIVTKKSIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWSIDDFER 296 ********************************************************************** PP TIGR00110 281 lsrkvPllaklkPsgkkviedlhraGGvsavlkeldkegllhkdaltvtGktlaetlekvkvlr.vdqdv 349 +++kvP++++lkPsg+ v +dlh+aGG+++v+k l +gllh d++t+tG+t+ae+l++v+ ++ +dq+v lcl|FitnessBrowser__BFirm:BPHYT_RS04815 297 MRKKVPVICNLKPSGQFVATDLHKAGGIPQVMKILLDAGLLHGDCITITGRTIAEELKDVPGKPrADQQV 366 ************************************************************988638999* PP TIGR00110 350 irsldnpvkkegglavLkGnlaeeGavvkiagveedilkfeGpakvfeseeealeailggkvkeGdvvvi 419 i ++d++++keg+la+LkGnla +Gav+ki+g ++ +++Gpa+vf++e+ aleail+ k+ +Gdvvv+ lcl|FitnessBrowser__BFirm:BPHYT_RS04815 367 IFPIDQALYKEGHLAILKGNLAVDGAVAKITGLKN--PVITGPARVFDDEQSALEAILADKIVAGDVVVL 434 ***********************************..999****************************** PP TIGR00110 420 ryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtrGlsiGhvsPeaaegGaialvedGDkik 489 ry GPkGgPGm+emLaPtsa++g GLg++v+LitDGrfsGgt G+++Ghv+Pea +gG+ia+v++GD i+ lcl|FitnessBrowser__BFirm:BPHYT_RS04815 435 RYLGPKGGPGMPEMLAPTSAIIGKGLGESVGLITDGRFSGGTWGMVVGHVAPEAFVGGTIAFVQEGDSIT 504 ********************************************************************** PP TIGR00110 490 iDienrkldlevseeelaerrakakkkearevkgaLakyaklvssadkGavl 541 iD+++ l+l+++++el++rra+++++++r+++g++aky l++ a+kGa++ lcl|FitnessBrowser__BFirm:BPHYT_RS04815 505 IDAHKLLLQLNIDNAELERRRAAWQQPKPRYTRGVMAKYFALAQPANKGAIT 556 **************************************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (557 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 9.92 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory