Alignments for a candidate for cysK in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for cysK in Bacteroides thetaiotaomicron VPI-5482

Align cysteine synthase (EC 2.5.1.47) (characterized)
to candidate 352607 BT3080 cysteine synthase A (NCBI ptt file)

Query= BRENDA::P9WP55
         (310 letters)



>FitnessBrowser__Btheta:352607
          Length = 315

 Score =  377 bits (968), Expect = e-109
 Identities = 191/306 (62%), Positives = 234/306 (76%), Gaps = 2/306 (0%)

Query: 3   IAEDITQLIGRTPLVRLRRV--TDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGL 60
           IA  +T L+G TPL+ L     ++G  A ++ K+E FNPA SVKDR+ +AM++ AE  G+
Sbjct: 4   IARKLTDLVGNTPLLELSNYNKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEMKGV 63

Query: 61  IKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGAD 120
           + P   I+EPTSGNTG+ LA V AA+GY+ +LTMP+TMS+ERR LL+A GAEL+LTPGAD
Sbjct: 64  LTPGATIIEPTSGNTGVGLAFVAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTPGAD 123

Query: 121 GMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDTDGKVDIVVAGVGTG 180
           GM GAIAKAEEL        + QQFENPANPA+H  TT  E+WRDT+GKVDI VAGVGTG
Sbjct: 124 GMKGAIAKAEELKAATPGSVILQQFENPANPAMHLRTTGLEIWRDTEGKVDIFVAGVGTG 183

Query: 181 GTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI 240
           GTI+GV + +K R PS + VAVEPA SPVLSGG+ GPH IQGIGAGFVP   +  +VDEI
Sbjct: 184 GTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTYNASVVDEI 243

Query: 241 ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYL 300
           I V N+DA+  +R LA EEGLLVGISSGAA  AA ++A+RPEN GK+IV +LPD GERYL
Sbjct: 244 IQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPENEGKMIVALLPDTGERYL 303

Query: 301 STPLFA 306
           ST L+A
Sbjct: 304 STILYA 309


Lambda     K      H
   0.318    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 348
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 310
Length of database: 315
Length adjustment: 27
Effective length of query: 283
Effective length of database: 288
Effective search space:    81504
Effective search space used:    81504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate 352607 BT3080 (cysteine synthase A (NCBI ptt file))
to HMM TIGR01139 (cysK: cysteine synthase A (EC 2.5.1.47))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01139.hmm
# target sequence database:        /tmp/gapView.24082.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01139  [M=298]
Accession:   TIGR01139
Description: cysK: cysteine synthase A
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
   2.5e-144  466.0   0.8   2.9e-144  465.8   0.8    1.0  1  lcl|FitnessBrowser__Btheta:352607  BT3080 cysteine synthase A (NCBI


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Btheta:352607  BT3080 cysteine synthase A (NCBI ptt file)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  465.8   0.8  2.9e-144  2.9e-144       2     298 .]       9     308 ..       8     308 .. 0.98

  Alignments for each domain:
  == domain 1  score: 465.8 bits;  conditional E-value: 2.9e-144
                          TIGR01139   2 seliGntPlvrLn...laeeakaevlvkleslnPsssvkdrialamiedaekegllkkgktiveatsGntGialam 74 
                                        ++l+GntPl++L      ++ ka+++vk+es+nP++svkdr+alamiedae +g+l++g ti+e+tsGntG++la+
  lcl|FitnessBrowser__Btheta:352607   9 TDLVGNTPLLELSnynKSNGLKARLIVKIESFNPAGSVKDRVALAMIEDAEMKGVLTPGATIIEPTSGNTGVGLAF 84 
                                        689*********8655677899****************************************************** PP

                          TIGR01139  75 vaaargykliltmpetmslerrkllkayGaelvLtdgaegmkgaiekaeelveetpnkylllkqfenpanpeihrk 150
                                        vaaa+gykliltmp+tms+err+llka+GaelvLt+ga gmkgai+kaeel ++tp +  +l+qfenpanp++h +
  lcl|FitnessBrowser__Btheta:352607  85 VAAAKGYKLILTMPDTMSVERRNLLKALGAELVLTPGADGMKGAIAKAEELKAATPGSV-ILQQFENPANPAMHLR 159
                                        ********************************************************666.**************** PP

                          TIGR01139 151 ttapeilkdldgkldafvagvGtGGtitGvgevlkekkpdikvvavePaespvlsggkpgphkiqGigagfiPkvL 226
                                        tt+ ei++d++gk+d+fvagvGtGGti+Gvge+lk  +p++k+vavePa+spvlsggkpgphkiqGigagf+Pk  
  lcl|FitnessBrowser__Btheta:352607 160 TTGLEIWRDTEGKVDIFVAGVGTGGTISGVGEALKMRDPSVKAVAVEPADSPVLSGGKPGPHKIQGIGAGFVPKTY 235
                                        **************************************************************************** PP

                          TIGR01139 227 dkevidevikvsdeeaietarrlakeeGilvGissGaavaaalkvakkle.kdkkivvilpdtgerYlstaLf 298
                                        + +v+de+i+v++++ai+t+r la+eeG+lvGissGaav+aa+++ak++e ++k+iv++lpdtgerYlst L+
  lcl|FitnessBrowser__Btheta:352607 236 NASVVDEIIQVQNDDAIRTSRALAEEEGLLVGISSGAAVYAATELAKRPEnEGKMIVALLPDTGERYLSTILY 308
                                        **************************************************99******************998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (315 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 10.73
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis