Alignments for a candidate for prs in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for prs in Bacteroides thetaiotaomicron VPI-5482

Align ribose-phosphate diphosphokinase (EC 2.7.6.1) (characterized)
to candidate 350276 BT0748 ribose-phosphate pyrophosphokinase (NCBI ptt file)

Query= BRENDA::E7EAU9
         (317 letters)



>FitnessBrowser__Btheta:350276
          Length = 305

 Score =  266 bits (679), Expect = 6e-76
 Identities = 142/302 (47%), Positives = 201/302 (66%), Gaps = 3/302 (0%)

Query: 12  IFSLNSNPELAKEIADNVGVQLGKCSVTRFSDGEVQINIEESIRGCDCYIIQSTSAPVNE 71
           +FS  ++  LA++I  ++   LG  ++T F+DGE  ++ EESIRG   +++QST  P ++
Sbjct: 2   VFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLVQSTF-PNSD 60

Query: 72  HIMELLIMVDALKRASAKTINIVIPYYGYARQDRKARSREPITAKLFANLLETAGATRVI 131
           ++MELL+M+DA KRASAK++  VIPY+G+ARQDRK + R  I AKL A+LL  AG  R+I
Sbjct: 61  NLMELLLMIDAAKRASAKSVVAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLI 120

Query: 132 ALDLHAPQIQGFFDIPIDHLMGVPILGHYFEGKDLKDIVIVSPDHGGVTRARKLADRLKA 191
            +DLHA QIQGFF+IP+DHL    +   Y +   L+D+VI +PD GG  RA   +  L  
Sbjct: 121 TMDLHADQIQGFFNIPVDHLYASAVFLPYIQSLKLEDLVIATPDVGGSKRASTFSKYLGV 180

Query: 192 PIAIIDKRRPRPNEVEVMNIVGNVEGKTAILIDDIIDTAGTITLAANALVENGAAEVYAC 251
           P+ + +K R + NEV  M I+G+V+ K  +LIDDI+DTAGTIT AAN ++E GA  V A 
Sbjct: 181 PLVLCNKSREKANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKAANIMMEAGAKSVRAI 240

Query: 252 CTHPVLSGPAVERINNSKIKELVVTNSIKLPEEKKIERFKQLSVGPLLAEAIIRVHEKQS 311
            +H V+S PA  R+  S + E+V T+SI  P  KK  + KQLS+  + AE I RV   +S
Sbjct: 241 ASHCVMSDPASFRVQESGLTEMVFTDSI--PYAKKCAKVKQLSIADMFAETIKRVMNNES 298

Query: 312 VS 313
           +S
Sbjct: 299 IS 300


Lambda     K      H
   0.319    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 196
Number of extensions: 4
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 317
Length of database: 305
Length adjustment: 27
Effective length of query: 290
Effective length of database: 278
Effective search space:    80620
Effective search space used:    80620
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate 350276 BT0748 (ribose-phosphate pyrophosphokinase (NCBI ptt file))
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.19450.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
   4.9e-111  356.5   1.1   5.6e-111  356.3   1.1    1.0  1  lcl|FitnessBrowser__Btheta:350276  BT0748 ribose-phosphate pyrophos


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Btheta:350276  BT0748 ribose-phosphate pyrophosphokinase (NCBI ptt file)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  356.3   1.1  5.6e-111  5.6e-111       2     306 ..       1     301 [.       1     304 [. 0.98

  Alignments for each domain:
  == domain 1  score: 356.3 bits;  conditional E-value: 5.6e-111
                          TIGR01251   2 kilsgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmellllidalkr 77 
                                        +++sg++s+ laek++ +l+ +lg++++++FadgE+ v +ees+rg +vf++ qst  p +d+lmelll+ida+kr
  lcl|FitnessBrowser__Btheta:350276   1 MVFSGTNSRYLAEKICASLNCPLGNMNITHFADGEFAVSYEESIRGAHVFLV-QSTF-PNSDNLMELLLMIDAAKR 74 
                                        689*************************************************.7777.79**************** PP

                          TIGR01251  78 asaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiqgfFdvpvenlsaspklieel 153
                                        asaksv aviPy+g+aRqd+k+k+r +i aklva+ll+ aG+dr++t+dlH++qiqgfF++pv++l+as +++ ++
  lcl|FitnessBrowser__Btheta:350276  75 ASAKSVVAVIPYFGWARQDRKDKPRVSIGAKLVADLLSVAGIDRLITMDLHADQIQGFFNIPVDHLYASAVFLPYI 150
                                        **************************************************************************** PP

                          TIGR01251 154 kkkelknlvvvsPDkGaverakkvakklglelaiieKeRdskenevevtnllgdvegkdvvivDDiisTggTlvka 229
                                        ++ +l++lv+  PD G+ +ra++++k lg++l++ +K R+ k+nev +++++gdv++k+vv++DDi++T+gT++ka
  lcl|FitnessBrowser__Btheta:350276 151 QSLKLEDLVIATPDVGGSKRASTFSKYLGVPLVLCNKSRE-KANEVASMQIIGDVKDKNVVLIDDIVDTAGTITKA 225
                                        ****************************************.899******************************** PP

                          TIGR01251 230 aelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilveekklpkvseisvapliaeaiarihenesvs 305
                                        a+++ e+GAk+v ++a+h v+s++A  r++e+g++e++ t++i+   kk+ kv+++s+a+++ae+i+r+ +nes+s
  lcl|FitnessBrowser__Btheta:350276 226 ANIMMEAGAKSVRAIASHCVMSDPASFRVQESGLTEMVFTDSIPY-AKKCAKVKQLSIADMFAETIKRVMNNESIS 300
                                        *********************************************.99***************************9 PP

                          TIGR01251 306 s 306
                                        s
  lcl|FitnessBrowser__Btheta:350276 301 S 301
                                        8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (305 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.73
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis