GapMind for Amino acid biosynthesis

 

Finding step leuC for L-leucine biosynthesis in Bacteroides thetaiotaomicron VPI-5482

2 candidates for leuC: 3-isopropylmalate dehydratase, large subunit

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi BT1860 3-isopropylmalate dehydratase large subunit (NCBI ptt file) 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 (characterized) 54% 99% 495.7 Homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 33% 209.1
hi BT1860 3-isopropylmalate dehydratase large subunit (NCBI ptt file) leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33) (TIGR00170) 99% 642.1 Homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 33% 209.1
lo BT2072 aconitate hydratase (NCBI ptt file) 3-isopropylmalate dehydratase large subunit 2; EC 4.2.1.33; Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 (uncharacterized) 33% 100% 208.4 aconitate hydratase (EC 4.2.1.3) 85% 1314.7

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

Also see fitness data for the candidates

Definition of step leuC

Or cluster all characterized leuC proteins

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory