Align candidate 349777 BT0249 (5-methyltetrahydrofolate--homocysteine methyltransferase or methionine synthase (NCBI ptt file))
to HMM PF02965 (Met_synt_B12)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/PF02965.21.hmm # target sequence database: /tmp/gapView.27873.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: Met_synt_B12 [M=273] Accession: PF02965.21 Description: Vitamin B12 dependent methionine synthase, activation domain Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-103 331.8 0.0 2e-103 331.6 0.0 1.0 1 lcl|FitnessBrowser__Btheta:349777 BT0249 5-methyltetrahydrofolate- Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Btheta:349777 BT0249 5-methyltetrahydrofolate--homocysteine methyltransferase or methionine synt # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 331.6 0.0 2e-103 2e-103 2 273 .] 7 285 .. 6 285 .. 0.98 Alignments for each domain: == domain 1 score: 331.6 bits; conditional E-value: 2e-103 Met_synt_B12 2 leelveyidWtpffqaWelkgkypkile..................dekvgeeakklfkdAqamLkkiieekllka 59 ++++++yi+W +ff+aW+++ ++++i++ d+++++ea++lfk+A++mL+ + ++ ++ lcl|FitnessBrowser__Btheta:349777 7 IHNVAPYINWIYFFHAWGFQPRFAAIANihgcdvcraswlttfpeeDRNKASEAMQLFKEANRMLDLLDKDY--EV 80 899****************************************************************98877..99 PP Met_synt_B12 60 kavvglfpAnsegddievyadesrseelatlhtLrqqaekeegkpnlclaDfvapkesgvkDyiGlFavtaglgie 135 + +++l++Ans+gd++++ e+++++ ++++Lrqq+ k++g+p +cl+Df++p +sg+ D+iG+Fa ++++++e lcl|FitnessBrowser__Btheta:349777 81 RTIFKLCKANSDGDNLVI---EKETDQFLVFPLLRQQTPKRDGSPFFCLSDFIRPLSSGIPDTIGAFASCIDADME 153 ******************...56667899*********************************************** PP Met_synt_B12 136 elakefeaekddYsailvkaladrLaeAfaellhekvrkelWgyakdeklsneelikekYqgiRpApGYpacpdht 211 l++ +d Y+++lv++l+drLaeA++e++he vrke Wgyakde ls+ +l+ ekYqgiRpA+GYp++pd++ lcl|FitnessBrowser__Btheta:349777 154 GLYE-----QDPYKHLLVQTLSDRLAEAATEKMHEYVRKEAWGYAKDEILSMPDLLVEKYQGIRPAVGYPSLPDQS 224 **98.....7****************************************************************** PP Met_synt_B12 212 ekktlfelldaeekigieLteslamtPaasvsGlyfahpearyFavgkiekdqvedyakrkg 273 ++ l elld+++ igi+Lte+ am+P+asv+Gl+fahp+a+yF+vgki++dq+edya+r+g lcl|FitnessBrowser__Btheta:349777 225 INFLLDELLDMKQ-IGITLTENGAMYPHASVCGLMFAHPAAEYFSVGKIGEDQLEDYARRRG 285 *************.**********************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (273 nodes) Target sequences: 1 (300 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00 # Mc/sec: 8.21 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory