Alignments for a candidate for metA in Bacteroides thetaiotaomicron VPI-5482

GapMind for Amino acid biosynthesis

Alignments for a candidate for metA in Bacteroides thetaiotaomicron VPI-5482

Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate 351941 BT2413 homoserine O-succinyltransferase (NCBI ptt file)

Query= SwissProt::Q72X44
         (301 letters)



>FitnessBrowser__Btheta:351941
          Length = 305

 Score =  379 bits (973), Expect = e-110
 Identities = 174/299 (58%), Positives = 227/299 (75%)

Query: 1   MPIIIDKDLPARKVLQEENIFVMTKERAETQDIRALKIAILNLMPTKQETEAQLLRLIGN 60
           MP+ +   LPA ++L+EENIFV+   RA  QDIR L+I ILNLMP K  TE  L+RL+ N
Sbjct: 1   MPLNLPDKLPAIELLKEENIFVIDTSRATQQDIRPLRIVILNLMPLKITTETDLVRLLSN 60

Query: 61  TPLQLDVHLLHMESHLSRNVAQEHLTSFYKTFRDIENEKFDGLIITGAPVETLSFEEVDY 120
           TPLQ+++  + ++SH S+N   EH+ +FY  F  + NEK+DG+IITGAPVE + FEEV Y
Sbjct: 61  TPLQVEISFMKIKSHTSKNTPIEHMQTFYTDFEQMRNEKYDGMIITGAPVEQMDFEEVTY 120

Query: 121 WEELKRIMEYSKTNVTSTLHICWGAQAGLYHHYGVQKYPLKEKMFGVFEHEVREQHVKLL 180
           WEE+  I ++++T+VTSTL+ICW AQAGLYHHYGV KYPL +KMFG+FEH V E    + 
Sbjct: 121 WEEITEIFDWARTHVTSTLYICWAAQAGLYHHYGVPKYPLDQKMFGIFEHRVLEPFHSIF 180

Query: 181 QGFDELFFAPHSRHTEVRESDIREVKELTLLANSEEAGVHLVIGQEGRQVFALGHSEYSC 240
           +GFD+ F+ PHSRHTEVR  DI +V ELTLL+ S++AGV++ + + GR+ F  GHSEYS 
Sbjct: 181 RGFDDCFYVPHSRHTEVRREDILKVPELTLLSESKDAGVYMAMARGGREFFVTGHSEYSP 240

Query: 241 DTLKQEYERDRDKGLNIDVPKNYFKHDNPNEKPLVRWRSHGNLLFSNWLNYYVYQETPY 299
            TL  EY RD  KGL I++P+NY+  D+P++ PLVRWR+H NLLFSNWLNY+VYQETPY
Sbjct: 241 YTLDTEYRRDLGKGLPIEIPRNYYVDDDPDKGPLVRWRAHANLLFSNWLNYFVYQETPY 299


Lambda     K      H
   0.319    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 319
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 301
Length of database: 305
Length adjustment: 27
Effective length of query: 274
Effective length of database: 278
Effective search space:    76172
Effective search space used:    76172
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate 351941 BT2413 (homoserine O-succinyltransferase (NCBI ptt file))
to HMM TIGR01001 (metA: homoserine O-succinyltransferase (EC 2.3.1.46))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01001.hmm
# target sequence database:        /tmp/gapView.13495.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01001  [M=301]
Accession:   TIGR01001
Description: metA: homoserine O-succinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
   6.5e-142  458.4   0.2   7.4e-142  458.3   0.2    1.0  1  lcl|FitnessBrowser__Btheta:351941  BT2413 homoserine O-succinyltran


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Btheta:351941  BT2413 homoserine O-succinyltransferase (NCBI ptt file)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  458.3   0.2  7.4e-142  7.4e-142       1     300 [.       1     301 [.       1     302 [. 1.00

  Alignments for each domain:
  == domain 1  score: 458.3 bits;  conditional E-value: 7.4e-142
                          TIGR01001   1 mpirvpdelpavellkeenifvmtekrashqdirplevlilnlmpkkietenqllrllsnsplqvditllridsrk 76 
                                        mp+++pd+lpa+ellkeenifv+ ++ra++qdirpl+++ilnlmp ki te+ l+rllsn+plqv+i +++i+s++
  lcl|FitnessBrowser__Btheta:351941   1 MPLNLPDKLPAIELLKEENIFVIDTSRATQQDIRPLRIVILNLMPLKITTETDLVRLLSNTPLQVEISFMKIKSHT 76 
                                        9*************************************************************************** PP

                          TIGR01001  77 skntpiehlekfykeleevkdrkfdGlivtGapvellefedvayweelkeilewskenvtstlyicwaaqaalkll 152
                                        skntpieh+++fy+++e+++++k+dG+i+tGapve+++fe+v ywee++ei++w++++vtstlyicwaaqa+l+  
  lcl|FitnessBrowser__Btheta:351941  77 SKNTPIEHMQTFYTDFEQMRNEKYDGMIITGAPVEQMDFEEVTYWEEITEIFDWARTHVTSTLYICWAAQAGLYHH 152
                                        **************************************************************************** PP

                          TIGR01001 153 ygipkrtleeklsGvykhdiv.kedlllrgfddkflaphsryadldeeliaeltdleilaesdeagvylaaskder 227
                                        yg+pk+ l++k++G+++h+++ +++ ++rgfdd f++phsr++++ +e+i ++++l +l es++agvy+a+++  r
  lcl|FitnessBrowser__Btheta:351941 153 YGVPKYPLDQKMFGIFEHRVLePFHSIFRGFDDCFYVPHSRHTEVRREDILKVPELTLLSESKDAGVYMAMARGGR 228
                                        ********************999***************************************************** PP

                          TIGR01001 228 nifvtGhpeydketlrqeyvrdvgeglkvdipknyypkddpektpiaswrshanllfanwlnyavyqktpydl 300
                                        + fvtGh ey  +tl  ey rd+g+gl ++ip nyy +ddp+k p + wr+hanllf+nwlny vyq+tpy++
  lcl|FitnessBrowser__Btheta:351941 229 EFFVTGHSEYSPYTLDTEYRRDLGKGLPIEIPRNYYVDDDPDKGPLVRWRAHANLLFSNWLNYFVYQETPYNI 301
                                        ***********************************************************************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (301 nodes)
Target sequences:                          1  (305 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.27
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis