Align Serine hydroxymethyltransferase 2; SHMT 2; Serine methylase 2; EC 2.1.2.1 (characterized)
to candidate H281DRAFT_00990 H281DRAFT_00990 serine hydroxymethyltransferase (EC 2.1.2.1)
Query= SwissProt::Q3JGP5 (424 letters) >FitnessBrowser__Burk376:H281DRAFT_00990 Length = 431 Score = 631 bits (1628), Expect = 0.0 Identities = 312/418 (74%), Positives = 351/418 (83%) Query: 7 FFSQSLAERDASVRGAILKELERQQSQVELIASENIVSRAVLDAQGSVLTNKYAEGYPGK 66 FF++ L RD + I EL RQQ+Q+ELIASENIVS AV++AQG+VLTNKYAEGYP K Sbjct: 7 FFAEPLQSRDPVIASEIALELRRQQTQIELIASENIVSAAVMEAQGTVLTNKYAEGYPSK 66 Query: 67 RYYGGCEFADEVEALAIERVKRLFNAGHANVQPHSGAQANGAVMLALAKPGDTVLGMSLD 126 RYYGGCE D VEALAI+RVK LF A ANVQPHSGAQANGAVMLAL KPG+TV+GMSLD Sbjct: 67 RYYGGCEHVDRVEALAIDRVKALFEAEFANVQPHSGAQANGAVMLALVKPGETVMGMSLD 126 Query: 127 AGGHLTHGAKPALSGKWFNALQYGVSRDTMLIDYDQVEALAQQHKPSLIIAGFSAYPRKL 186 AGGHLTHGA+PALSGKWFNA+QYGV+ T +DY+QV +LAQ+H+P LIIAG+SAYPR L Sbjct: 127 AGGHLTHGARPALSGKWFNAVQYGVNPQTYRVDYEQVRSLAQEHRPKLIIAGYSAYPRAL 186 Query: 187 DFARFRAIADSVGAKLMVDMAHIAGVIAAGRHANPVEHAHVVTSTTHKTLRGPRGGFVLT 246 DFA FR IADSVGA LMVDMAHIAG++AAGRH NPV A VVTSTTHKTLRGPRGGF+LT Sbjct: 187 DFAAFRDIADSVGALLMVDMAHIAGIVAAGRHENPVRFADVVTSTTHKTLRGPRGGFILT 246 Query: 247 NDEEIAKKINSAVFPGLQGGPLMHVIAGKAVAFGEALTDDFKTYIDRVLANAQALGDVLK 306 N+ +IAKKINSAVFPGLQGGPLMHVIAGKAVAFGEAL +F YID+VL NAQALGDVLK Sbjct: 247 NNGDIAKKINSAVFPGLQGGPLMHVIAGKAVAFGEALRPEFTAYIDQVLRNAQALGDVLK 306 Query: 307 AGGVDLVTGGTDNHLLLVDLRPKGLKGAQVEQALERAGITCNKNGIPFDPEKPTITSGIR 366 +GG+ LVT GTDNHLLLVDLR K L G Q E+ALERAGITCNKNGIPFD E PT+TSGIR Sbjct: 307 SGGLSLVTDGTDNHLLLVDLRSKRLTGTQAEKALERAGITCNKNGIPFDTESPTVTSGIR 366 Query: 367 LGTPAGTTRGFGAAEFREVGRLILEVFEALRTNPEGDHATEQRVRREIFALCERFPIY 424 LGTPAGTTRGFG A+F ++G++ILEV AL P GD E+ VR + LC +FPIY Sbjct: 367 LGTPAGTTRGFGTAQFEQIGQMILEVLSALEQEPAGDEQVERAVRSRVRDLCSQFPIY 424 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 660 Number of extensions: 24 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 424 Length of database: 431 Length adjustment: 32 Effective length of query: 392 Effective length of database: 399 Effective search space: 156408 Effective search space used: 156408 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory