GapMind for Amino acid biosynthesis


L-histidine biosynthesis in Paraburkholderia bryophila 376MFSha3.1

Best path

prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC, hisN, hisD

Also see fitness data for the top candidates


Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).

11 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
prs ribose-phosphate diphosphokinase H281DRAFT_01939
hisG ATP phosphoribosyltransferase H281DRAFT_05651
hisI phosphoribosyl-ATP pyrophosphatase H281DRAFT_05661 H281DRAFT_05652
hisE phosphoribosyl-AMP cyclohydrolase H281DRAFT_05660 H281DRAFT_05652
hisA isomerase HisA H281DRAFT_05658 H281DRAFT_05659
hisF IGP synthase, cyclase subunit H281DRAFT_05659 H281DRAFT_05658
hisH IGP synthase, amidotransferase subunit H281DRAFT_05657
hisB IGP dehydratase H281DRAFT_05655
hisC histidinol-phosphate aminotransferase H281DRAFT_01942 H281DRAFT_05654
hisN histidinol-phosphate phosphatase H281DRAFT_04707 H281DRAFT_02047
hisD histidinol dehydrogenase H281DRAFT_05652

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory