Alignments for a candidate for leuC in Paraburkholderia bryophila 376MFSha3.1

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuC in Paraburkholderia bryophila 376MFSha3.1

Align 3-isopropylmalate dehydratase large subunit 1; Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1; EC 4.2.1.33 (characterized)
to candidate H281DRAFT_02541 H281DRAFT_02541 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33)

Query= SwissProt::P15717
         (466 letters)



>FitnessBrowser__Burk376:H281DRAFT_02541
          Length = 465

 Score =  476 bits (1224), Expect = e-139
 Identities = 238/466 (51%), Positives = 310/466 (66%), Gaps = 6/466 (1%)

Query: 1   MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT 60
           MA+TL+++++DAHVV +      LL IDRHL+H+++ P  F+ L A   PV +P  +FAT
Sbjct: 1   MAQTLFDRIWDAHVVTDLGGGWALLAIDRHLLHDLSGPGGFETLAARELPVSRPDLSFAT 60

Query: 61  MDHNVST---QTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGV 117
            DH VS+   +T + N +G      ++     C+   V  YDL    QGIVHVM PE G+
Sbjct: 61  ADHAVSSAPHRTGETNPAGAKLYASLRRF---CDSASVPFYDLGKAGQGIVHVMAPEMGL 117

Query: 118 TLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPG 177
           TLPG TIVCGDSHT THG  GALAFGIG+SE+ HVLATQT++Q + + M+I  TG  A G
Sbjct: 118 TLPGTTIVCGDSHTCTHGGLGALAFGIGSSELAHVLATQTVRQKKPRQMRITYTGVLAKG 177

Query: 178 ITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDE 237
           + AKD+ L +I K G + G G+ +E CG A++ LS+E R+TLCNM++E+G+K     PD+
Sbjct: 178 VFAKDLALHVIAKLGCSAGVGYAIEHCGPALKRLSVEERLTLCNMSVELGSKIAFCPPDD 237

Query: 238 TTFNYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQV 297
            TF++++G  +AP G +F+ AV  W+ L++DD A FD  V++   ++ P VTWGT P   
Sbjct: 238 VTFDFLRGLPYAPAGDEFEHAVSEWRKLRSDDNAHFDVEVSIDLSDVEPMVTWGTTPEHA 297

Query: 298 ISVTDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRA 357
           ++VT  +PDP   +D   R   E AL YMGL  G  L   ++D VFIGSC NSRI DLR 
Sbjct: 298 LAVTSAVPDPTDSTDAERRKGVEAALEYMGLCGGQSLAGTSVDWVFIGSCANSRISDLRE 357

Query: 358 AAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDR 417
           AA + +G+ VAP VQA VVPGS  VK  AEAEGL  +F  AGFEWR PGCSMC+A N D 
Sbjct: 358 AASIVRGKHVAPNVQAWVVPGSQLVKEIAEAEGLADVFRSAGFEWREPGCSMCVAANGDV 417

Query: 418 LNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIR 463
           + PG+R  STSNRNF GRQG G RTHL SPA AAAAA+ G   D R
Sbjct: 418 VPPGQRIVSTSNRNFIGRQGPGARTHLASPATAAAAAIAGAIVDPR 463


Lambda     K      H
   0.318    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 672
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 466
Length of database: 465
Length adjustment: 33
Effective length of query: 433
Effective length of database: 432
Effective search space:   187056
Effective search space used:   187056
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate H281DRAFT_02541 H281DRAFT_02541 (3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00170.hmm
# target sequence database:        /tmp/gapView.26635.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00170  [M=466]
Accession:   TIGR00170
Description: leuC: 3-isopropylmalate dehydratase, large subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                    Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                    -----------
   4.4e-184  598.3   0.0   5.3e-184  598.1   0.0    1.0  1  lcl|FitnessBrowser__Burk376:H281DRAFT_02541  H281DRAFT_02541 3-isopropylmalat


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Burk376:H281DRAFT_02541  H281DRAFT_02541 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.3
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  598.1   0.0  5.3e-184  5.3e-184       1     464 [.       1     463 [.       1     464 [. 0.99

  Alignments for each domain:
  == domain 1  score: 598.1 bits;  conditional E-value: 5.3e-184
                                    TIGR00170   1 maktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnis 66 
                                                  ma+tl+++++dahvv +  ++  ll idrhl+h+++ p  fe l a++ +v r+d ++at dh++s
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541   1 MAQTLFDRIWDAHVVTDLGGGWALLAIDRHLLHDLSGPGGFETLAARELPVSRPDLSFATADHAVS 66 
                                                  89**************************************************************** PP

                                    TIGR00170  67 tesrdveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshta 132
                                                  +  + +  ++        +l + ++   v+++dl +a qgivhv+ pe gltlpg+tivcgdsht+
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541  67 SAPHRTGETNPAGAKLYASLRRFCDSASVPFYDLGKAGQGIVHVMAPEMGLTLPGTTIVCGDSHTC 132
                                                  9988787777777777899*********************************************** PP

                                    TIGR00170 133 thgafgalafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtg 198
                                                  thg +galafgig+se+ hvlatqt++q+++++++i+  g lakg+ akd+ l +i k+g + g g
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 133 THGGLGALAFGIGSSELAHVLATQTVRQKKPRQMRITYTGVLAKGVFAKDLALHVIAKLGCSAGVG 198
                                                  ****************************************************************** PP

                                    TIGR00170 199 yvvefageairdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywkt 264
                                                  y +e++g a++ ls+eer+t+cnm++e+g+k  +  pd++tf+++++ +yap g+efe+av++w+ 
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 199 YAIEHCGPALKRLSVEERLTLCNMSVELGSKIAFCPPDDVTFDFLRGLPYAPAGDEFEHAVSEWRK 264
                                                  ****************************************************************** PP

                                    TIGR00170 265 lktdegakfdkvvtleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglep 330
                                                  l++d++a+fd +v+++ +d+ p+vtwgt+p+++l+v++ vpdp + +d  +++++e al+y+gl  
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 265 LRSDDNAHFDVEVSIDLSDVEPMVTWGTTPEHALAVTSAVPDPTDSTDAERRKGVEAALEYMGLCG 330
                                                  ****************************************************************** PP

                                    TIGR00170 331 gtklkdikvdkvfigsctnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkif 396
                                                  g++l    vd vfigsc+nsri dlr+aa++v+gk+va+nv+ a vvpgs+lvk+ ae+egl  +f
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 331 GQSLAGTSVDWVFIGSCANSRISDLREAASIVRGKHVAPNVQ-AWVVPGSQLVKEIAEAEGLADVF 395
                                                  ******************************************.*********************** PP

                                    TIGR00170 397 leagfewreagcslclgmnndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvd 462
                                                    agfewre+gcs+c++ n dv+++++r  stsnrnf grqg garthl spa aaaaa+ag +vd
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 396 RSAGFEWREPGCSMCVAANGDVVPPGQRIVSTSNRNFIGRQGPGARTHLASPATAAAAAIAGAIVD 461
                                                  *****************************************************************9 PP

                                    TIGR00170 463 ir 464
                                                   r
  lcl|FitnessBrowser__Burk376:H281DRAFT_02541 462 PR 463
                                                  76 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (465 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.55
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis