Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate H281DRAFT_00811 H281DRAFT_00811 L-threonine synthase
Query= BRENDA::Q2SWH9 (483 letters) >FitnessBrowser__Burk376:H281DRAFT_00811 Length = 483 Score = 852 bits (2200), Expect = 0.0 Identities = 424/483 (87%), Positives = 451/483 (93%) Query: 1 MNYISTRGAGIGERHTFSDILLGGLAKDGGLYLPSEYPQVSADELARWRTLPYADLAFEI 60 MNY+STRGAG GERHTFSDILLGGLAKDGGLYLP+EYP+VSADELARWRTLPYADLAFEI Sbjct: 1 MNYLSTRGAGAGERHTFSDILLGGLAKDGGLYLPAEYPRVSADELARWRTLPYADLAFEI 60 Query: 61 LSKFCDDIAAADLRAITRRTYTADVYRHARRGGNAADITPLTTLGTENGAPVSLLELSNG 120 LSKF DDI A DLRA+TR+TYTA Y + R A ITPL TLG ENGAP+SLLELSNG Sbjct: 61 LSKFSDDIPAEDLRALTRKTYTAQTYCNVRDDEGAEQITPLKTLGIENGAPLSLLELSNG 120 Query: 121 PTLAFKDMAMQLLGNLFEYTLAKHGETLNILGATSGDTGSAAEYAMRGKEGVRVFMLSPH 180 PTLAFKDMAMQL+GNLFEY LAKHGETLNILGATSGDTGSAAEYAMRGK+G+RVFMLSPH Sbjct: 121 PTLAFKDMAMQLIGNLFEYALAKHGETLNILGATSGDTGSAAEYAMRGKQGIRVFMLSPH 180 Query: 181 KKMSAFQTAQMYSLQDPNIFNLAVNGVFDDCQDIVKAVSNDHAFKAQQKIGTVNSINWAR 240 KKMSAFQTAQMYSLQDPNIFNLAV GVFDDCQDIVKAVSNDHAFKA+ KIGTVNSINWAR Sbjct: 181 KKMSAFQTAQMYSLQDPNIFNLAVEGVFDDCQDIVKAVSNDHAFKAKYKIGTVNSINWAR 240 Query: 241 VVAQVVYYFKGYFAATRSNDERVSFTVPSGNFGNVCAGHIARMMGLPIEKLVVATNENDV 300 VVAQVVYYFKGYFAAT+SNDERVSFTVPSGNFGNVCAGHIARMMGLPIEKLVVATNENDV Sbjct: 241 VVAQVVYYFKGYFAATKSNDERVSFTVPSGNFGNVCAGHIARMMGLPIEKLVVATNENDV 300 Query: 301 LDEFFRTGAYRVRSAQDTYHTSSPSMDISKASNFERFVFDLLGRDPARVVQLFRDVEQKG 360 LDEFFRTG YRVR A +TYHTSSPSMDISKASNFERFVFDLLGRDPARV+QLFRDVE+KG Sbjct: 301 LDEFFRTGIYRVRKAAETYHTSSPSMDISKASNFERFVFDLLGRDPARVLQLFRDVEEKG 360 Query: 361 GFDLAASGDFARVAEFGFVSGRSTHADRIATIRDVFERYRTMIDTHTADGLKVAREHLRP 420 GFDLAASGDFARV EFGFVSGRS+H DR+ IRDVF+RY TMIDTHTADGLKVAREHL+P Sbjct: 361 GFDLAASGDFARVKEFGFVSGRSSHDDRVEAIRDVFQRYDTMIDTHTADGLKVAREHLQP 420 Query: 421 GVPMVVLETAQPIKFGESIREALGQEPSRPAAFDGLEALPQRFEVVDANAQQVKDFIAAH 480 GVPM+VLETAQPIKFGE+IREAL +EP RPAAF GLE+LPQRFEV+ A+AQ+VKDFI A+ Sbjct: 421 GVPMIVLETAQPIKFGETIREALLREPERPAAFTGLESLPQRFEVLPADAQRVKDFIVAN 480 Query: 481 TGA 483 TGA Sbjct: 481 TGA 483 Lambda K H 0.320 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 824 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 483 Length of database: 483 Length adjustment: 34 Effective length of query: 449 Effective length of database: 449 Effective search space: 201601 Effective search space used: 201601 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate H281DRAFT_00811 H281DRAFT_00811 (L-threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.17033.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.1e-72 228.3 0.0 9.6e-72 227.9 0.0 1.1 1 lcl|FitnessBrowser__Burk376:H281DRAFT_00811 H281DRAFT_00811 L-threonine synt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Burk376:H281DRAFT_00811 H281DRAFT_00811 L-threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 227.9 0.0 9.6e-72 9.6e-72 38 331 .. 110 443 .. 74 448 .. 0.87 Alignments for each domain: == domain 1 score: 227.9 bits; conditional E-value: 9.6e-72 TIGR00260 38 enlyvkelfhgPtlaFKDrglqfvavlltkalelgne..tvlcAtsGdtgaaaaealagkanvkvv 101 l ++el+ gPtlaFKD+++q +++l+++al + e +l+AtsGdtg+aa +a++gk++++v+ lcl|FitnessBrowser__Burk376:H281DRAFT_00811 110 APLSLLELSNGPTLAFKDMAMQLIGNLFEYALAKHGEtlNILGATSGDTGSAAEYAMRGKQGIRVF 175 458899************************99888775679************************* PP TIGR00260 102 vLyPkgkispv.keklvtalaenakvlaikGdFDdaqdlvkeifedke...klklnsvNsinpari 163 +L P+ k+s ++ + n+ la++G FDd+qd+vk++ +d+ k+k++ vNsin+ar+ lcl|FitnessBrowser__Burk376:H281DRAFT_00811 176 MLSPHKKMSAFqTAQMYSLQDPNIFNLAVEGVFDDCQDIVKAVSNDHAfkaKYKIGTVNSINWARV 241 ***********99*******************************9666677*************** PP TIGR00260 164 eaqktyafeiveqlgkespdkvvvpvpsgnfgailkGflekkelglpieklaiaaegaadivrrfl 229 aq++y+f + k + ++v + vpsgnfg++ +G ++++glpiekl +a++++ d++++f lcl|FitnessBrowser__Burk376:H281DRAFT_00811 242 VAQVVYYFKGYFAATKSNDERVSFTVPSGNFGNVCAG-HIARMMGLPIEKLVVATNEN-DVLDEFF 305 ****888876666666999******************.6667777************9.******* PP TIGR00260 230 ksg.dlepkedk..eTlstAmdignpsnverale.....larrslgnledlke............. 274 ++g + + k+ +T s++mdi++ sn+er ++ r l+ + d++e lcl|FitnessBrowser__Burk376:H281DRAFT_00811 306 RTGiYRVRKAAEtyHTSSPSMDISKASNFERFVFdllgrDPARVLQLFRDVEEkggfdlaasgdfa 371 ***64443333347899**************9998532223456667777777************* PP TIGR00260 275 ..........svsdeeileaikklaeeegyllephtavavaalkklvekg....vsatadpaKFee 326 + s ++ +eai+ + ++ ++++hta ++++ ++ ++ g v ta+p KF e lcl|FitnessBrowser__Burk376:H281DRAFT_00811 372 rvkefgfvsgRSSHDDRVEAIRDVFQRYDTMIDTHTADGLKVAREHLQPGvpmiVLETAQPIKFGE 437 **********99**************************************7654449*******98 PP TIGR00260 327 vve.al 331 ++ al lcl|FitnessBrowser__Burk376:H281DRAFT_00811 438 TIReAL 443 876555 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (483 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.61 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory