Aligments for a candidate for argD in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Aligments for a candidate for argD in Caulobacter crescentus NA1000

Align Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized)
to candidate CCNA_03233 CCNA_03233 Acetyl ornithine aminotransferase

Query= curated2:O30156
         (375 letters)



>FitnessBrowser__Caulo:CCNA_03233
          Length = 467

 Score =  169 bits (427), Expect = 2e-46
 Identities = 127/411 (30%), Positives = 199/411 (48%), Gaps = 60/411 (14%)

Query: 21  VIERGEGCYVYDVNGKRYLDLVAGIATVSIGHCNSHLVERLKEQLEKLIHISNLYYTT-- 78
           ++ R +GCY+ D +G R LD +AG+  V++G+    L +   EQ+ +L + +  + T   
Sbjct: 39  IVTRADGCYITDGDGHRILDGMAGLWCVNVGYGRKELADAAYEQMLELPYYNTFFKTATP 98

Query: 79  PQVELAEKLSEIAG--MDRFFFCNSGAEAVEAALKFARR------ATGRKKFVSFTGDFH 130
           P V+LA K++E  G  +   FF +SG+EA +   +  R          R  F+S    +H
Sbjct: 99  PTVKLAAKIAEKMGGHLTHVFFNSSGSEANDTVFRLVRHYWKLKGQPNRTVFISRWNAYH 158

Query: 131 GRTM--------------GALSVTHKEKFRKPFEPL-----VSPVEFAEFNNPESLEKVV 171
           G T+              G L +   E   +P+ P        P  F +    E  +K++
Sbjct: 159 GSTVAGVSLGGMKHMHVQGDLPIPGVEHVMQPY-PFGEGFGEDPAAFRDRAVKEIEDKIL 217

Query: 172 D---EETAAVIVELVQGEAGVYPADREFVKAIEELREKYGFLLIVDEVQTGFGRTGRWFA 228
           +   E  AA I E VQG  GV      +  A+E +  KYG LL+ DEV  GFGR G+WF 
Sbjct: 218 EVGPENVAAFIGEPVQGAGGVIIPPDGYWPAVEAVCRKYGILLVCDEVICGFGRLGQWFG 277

Query: 229 KDHYGIEPDMITMAKAMGSG-VPIGCCALKEEVAEKIQV--GD--HGSTFGGNPLACTAA 283
             HYGI+PD+I MAK + SG +PI    + + +  +++   GD  HG T+ G+P     A
Sbjct: 278 HQHYGIKPDLIAMAKGLSSGYLPISAVGVADHIVAELREKGGDFIHGFTYSGHPTCAAVA 337

Query: 284 LATIEVIEREGLVENSAR-MGEYFVKRLKESFENVI-----GVGLMIGFDV--------- 328
           L  IE+IERE L+  +    G Y  K L    ++ +      +GL+   ++         
Sbjct: 338 LKNIEIIEREDLITRTREDTGPYLAKALARLNDHPLVGETRSLGLIGAVEIVREKGTNHR 397

Query: 329 -----GDAAEFVRK-CLENGLLVNNTSERRIRLVPPLVITEREVDKAVEIM 373
                G+A   VR  C+ NGL+V    +  I   PPL+++  E+D+ V I+
Sbjct: 398 FLDKEGEAGPIVRDICIRNGLMVRAIRD-SIVCCPPLIVSHAEIDELVGII 447


Lambda     K      H
   0.320    0.138    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 453
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 467
Length adjustment: 32
Effective length of query: 343
Effective length of database: 435
Effective search space:   149205
Effective search space used:   149205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis