Alignments for a candidate for asd in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Caulobacter crescentus NA1000

Align Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 (characterized)
to candidate CCNA_00253 CCNA_00253 aspartate-semialdehyde dehydrogenase

Query= SwissProt::P23247
         (337 letters)



>FitnessBrowser__Caulo:CCNA_00253
          Length = 342

 Score =  310 bits (793), Expect = 4e-89
 Identities = 161/330 (48%), Positives = 226/330 (68%), Gaps = 4/330 (1%)

Query: 5   FNVAIFGATGAVGETMLEVLQEREFPVDELFLLASERSEGKTYRFNGKTVRVQNVEEFDW 64
           + VA+ GATG VG  M  +L+E +FPVD++  +AS +S G    F  + ++ +++E+FD+
Sbjct: 3   YRVAVVGATGNVGREMFNILEEVKFPVDKMHAIASRKSIGVEVSFGNEILKCEDLEQFDF 62

Query: 65  SQVHIALFSAGGELSAKWAPIAAEAGVVVIDNTSHFRYDYDIPLVVPEVNPEAIAEFRNR 124
           S+V + L SAGG +S  W      AG +VIDN+SHFR D D+PLVVPEVNPEA+     +
Sbjct: 63  SKVDLVLMSAGGSISKAWGEKIGAAGPIVIDNSSHFRMDPDVPLVVPEVNPEAVNSI-PK 121

Query: 125 NIIANPNCSTIQMLVALKPIYDAVGIERINVTTYQSVSGAGKAGIDELAGQTAKLLNGYP 184
           NIIANPNCST Q++VALKP++D   I+R+ V+TYQSVSGAGK G+DEL  QT  +     
Sbjct: 122 NIIANPNCSTAQLVVALKPLHDEAKIKRVVVSTYQSVSGAGKEGMDELWDQTKGVYVLGA 181

Query: 185 AETNTFSQQIAFNCIPQIDQFMDNGYTKEEMKMVWETQKIFNDPSIMVNPTCVRVPVFYG 244
                F++QIAFN IP ID FMD+G+TKEE KM  ETQKI  DP+I +  TCVRVPV  G
Sbjct: 182 PPPKKFTKQIAFNVIPHIDVFMDDGFTKEEWKMAVETQKIL-DPAIKLTATCVRVPVMVG 240

Query: 245 HAEAVHVETRAPIDAEQVMDMLEQTDGIELFRGADFPTQV--RDAGGKDHVLVGRVRNDI 302
           H+E+V+VE  +P+D ++  ++L + DGI +    +    +  +++ G+  V + R+R D 
Sbjct: 241 HSESVNVEFESPLDEDEAREILREADGIVVVDKREDGGYITPKESQGEFPVYISRIRKDP 300

Query: 303 SHHSGINLWVVADNVRKGAATNAVQIAELL 332
           +  +G+  W V+DN+RKGAA NAVQIA+LL
Sbjct: 301 TVENGLAFWCVSDNLRKGAALNAVQIAQLL 330


Lambda     K      H
   0.319    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 316
Number of extensions: 10
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 337
Length of database: 342
Length adjustment: 28
Effective length of query: 309
Effective length of database: 314
Effective search space:    97026
Effective search space used:    97026
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate CCNA_00253 CCNA_00253 (aspartate-semialdehyde dehydrogenase)
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.30053.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.9e-140  453.7   0.1   2.2e-140  453.5   0.1    1.0  1  lcl|FitnessBrowser__Caulo:CCNA_00253  CCNA_00253 aspartate-semialdehyd


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Caulo:CCNA_00253  CCNA_00253 aspartate-semialdehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  453.5   0.1  2.2e-140  2.2e-140       1     337 [.       4     331 ..       4     333 .. 0.98

  Alignments for each domain:
  == domain 1  score: 453.5 bits;  conditional E-value: 2.2e-140
                             TIGR01296   1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsaGgsv 73 
                                           +va+vGatG+vG+e+ ++Lee +fp+dk+ ++as++s G +v f +++l+ e++e+++f+++d+ l+saGgs+
  lcl|FitnessBrowser__Caulo:CCNA_00253   4 RVAVVGATGNVGREMFNILEEVKFPVDKMHAIASRKSIGVEVSFGNEILKCEDLEQFDFSKVDLVLMSAGGSI 76 
                                           69*********************************************************************** PP

                             TIGR01296  74 skefapkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkplkdeaklk 146
                                           sk++  k+ +ag iviDn+s fr+d+dvPLvvpevn e ++  + k+iianPnCst qlvv+Lkpl+deak+k
  lcl|FitnessBrowser__Caulo:CCNA_00253  77 SKAWGEKIGAAGPIVIDNSSHFRMDPDVPLVVPEVNPEAVNSIP-KNIIANPNCSTAQLVVALKPLHDEAKIK 148
                                           ****************************************9998.**************************** PP

                             TIGR01296 147 rvvvstYqavsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidklkedGytkeelkl 219
                                           rvvvstYq+vsGaGk+g++eL +qtk v++           ++kkf+kqiafn+ip+id +++dG+tkee k+
  lcl|FitnessBrowser__Caulo:CCNA_00253 149 RVVVSTYQSVSGAGKEGMDELWDQTKGVYVLGAP-------PPKKFTKQIAFNVIPHIDVFMDDGFTKEEWKM 214
                                           ****************************987544.......57****************************** PP

                             TIGR01296 220 lfetrkilgiedlkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvviddpsenlyptPleav 292
                                           + et+kil+ + +k++atcvrvPv++ghsesv++efe +l+ +e++e+L+ea+g+vv+d+ ++  y+tP e +
  lcl|FitnessBrowser__Caulo:CCNA_00253 215 AVETQKILD-PAIKLTATCVRVPVMVGHSESVNVEFESPLDEDEAREILREADGIVVVDKREDGGYITPKESQ 286
                                           *********.*************************************************************** PP

                             TIGR01296 293 gkdevfvgrirkDlskekglalfvvaDnlrkGaalnavqiaelli 337
                                           g+  v+++rirkD + e+gla ++v+DnlrkGaalnavqia+ll 
  lcl|FitnessBrowser__Caulo:CCNA_00253 287 GEFPVYISRIRKDPTVENGLAFWCVSDNLRKGAALNAVQIAQLLH 331
                                           *****************************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (342 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.69
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis