Aligments for a candidate for hom in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Aligments for a candidate for hom in Caulobacter crescentus NA1000

Align Homoserine dehydrogenase; EC 1.1.1.3 (characterized, see rationale)
to candidate CCNA_01447 CCNA_01447 homoserine dehydrogenase

Query= uniprot:A0A1L6J6Q3
         (430 letters)



>FitnessBrowser__Caulo:CCNA_01447
          Length = 429

 Score =  360 bits (923), Expect = e-104
 Identities = 194/422 (45%), Positives = 258/422 (61%), Gaps = 2/422 (0%)

Query: 6   RVALAGLGTVGAGVIRLIDANAELIARRAGRPIEIVAVSARDRAKDRGVDITRFDWVDDM 65
           RV +AGLGTVG G+++ +          AG    + AVSAR +++ R VDI+   W DD 
Sbjct: 7   RVGVAGLGTVGGGLLQFLAERPGFAP--AGDLAVVTAVSARSKSRPRTVDISNLVWFDDP 64

Query: 66  TELARHPKADVVVELIGGSDGPALALARATLAAGKGLVTANKAMIAHHGLELAQVAEKSD 125
             LA  P+ D+ VEL+GGSDGPA A     L AGK +VTANKA+IA HG ELA +AE ++
Sbjct: 65  VALAASPEIDIFVELVGGSDGPAKAAVETALKAGKPVVTANKALIAEHGAELAALAEATN 124

Query: 126 TPMKFEAAVAGGVPVIKGLREGAAANQIDRVYGILNGTCNFILSKMEAEGRDFGEVLAEA 185
           TP+ FEAAV GGVP +K +RE    + I  V GILNGTCNFILS+ME  GR F +VL EA
Sbjct: 125 TPLLFEAAVMGGVPAVKMMREALVGDDIISVAGILNGTCNFILSEMEKTGRSFADVLREA 184

Query: 186 QAAGFAEADPSFDIDGVDAAHKLSILASIAFGTQPAFGDVAIGGIRHLLAADIAEAAALG 245
           Q  G+AEADP+ D+ G DA HK+SILA++AFG  P F    I GI  +   DI  A  LG
Sbjct: 185 QGLGYAEADPTMDVGGFDAGHKVSILAALAFGCAPNFAAAEIEGISEVDLLDIKLAKDLG 244

Query: 246 YRIRLLGIADLSGNGLFQRVHPHLVPLSHPLAHVLGPTNAVVAEGNFVGRLLFQGAGAGD 305
           YRI+L+  A  S +G+  +VHP LVPL HPLA   G  NA+  EG  +GR+  QG GAG 
Sbjct: 245 YRIKLIAGAAKSEDGVAVKVHPSLVPLDHPLAQAGGALNALFIEGKRIGRIFLQGPGAGA 304

Query: 306 GPTASAVVADLIDIARTEFGPPYAMPATSLAAEPVAPTGERRGRAYLRFTVADKVGVLAE 365
           GPTA+AV AD+ D+      P +  PA  L         +  G+AYLR  V D+ GV+A 
Sbjct: 305 GPTAAAVAADIADVMTKAVRPVFQAPAGDLKPFVSIDPSKAVGKAYLRVMVQDQPGVIAA 364

Query: 366 IAAAMRDAGVSIESLIQRGAMADGSVLVAIVTHEVPERSIAQALEKLRGSPSLAGEPMWM 425
           I+  + + GVSI+S +Q+     G V + +VTH  PE ++  A+ ++    ++   P  +
Sbjct: 365 ISETLAECGVSIDSFLQKPVEGAGGVPIVLVTHATPESNLLDAISRIEKLQTVLERPRLL 424

Query: 426 HI 427
            +
Sbjct: 425 RV 426


Lambda     K      H
   0.319    0.136    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 496
Number of extensions: 18
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 429
Length adjustment: 32
Effective length of query: 398
Effective length of database: 397
Effective search space:   158006
Effective search space used:   158006
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis