GapMind for Amino acid biosynthesis

 

Aligments for a candidate for cysK in Echinicola vietnamensis KMM 6221, DSM 17526

Align Homocysteine/cysteine synthase; O-acetylserine/O-acetylhomoserine sulfhydrylase; OAS-OAH SHLase; OAS-OAH sulfhydrylase; EC 2.5.1.47; EC 2.5.1.49 (characterized)
to candidate Echvi_2283 Echvi_2283 Cystathionine beta-lyases/cystathionine gamma-synthases

Query= SwissProt::P06106
         (444 letters)



>lcl|FitnessBrowser__Cola:Echvi_2283 Echvi_2283 Cystathionine
           beta-lyases/cystathionine gamma-synthases
          Length = 391

 Score =  239 bits (609), Expect = 1e-67
 Identities = 143/434 (32%), Positives = 232/434 (53%), Gaps = 51/434 (11%)

Query: 4   HFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQLFGLEVPGYVYSRFQNPT 63
           HF+T  +           H   + PIY T+S+ F++++   Q+F  E+PG +YSR+ NP 
Sbjct: 5   HFETEAVRIASSKSNQREH---SAPIYMTSSFTFDSAEEARQMFAEEIPGNIYSRYANPN 61

Query: 64  SNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNIVSTSYLYGGTYNQFKISFK 123
           S+ L E++ A+EG    +A +SG AA   ++  L   GD+I+++  L+G T+      F 
Sbjct: 62  SSDLIEKVCAVEGTEDGIATASGMAAMFGSMASLLEQGDHILASRSLFGSTHQLLTRVFP 121

Query: 124 RFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNVPDFEKIVAIAHKHGIPVVVD 183
           ++GI + + +  + E ++K+    TK +++ET  NP   V D E I   A  H + +VVD
Sbjct: 122 KWGITSTYGDISDIENWDKLVKPNTKMLFIETPSNPGLEVIDLEWIGKFAKAHNLILVVD 181

Query: 184 NTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGIIVDSGKFPWKDYPEKFPQFSQP 243
           N F A  Y  QP K+GADIV HSATK+I G G  +GG+I+       K    K  QF   
Sbjct: 182 NCF-ATPYLQQPAKWGADIVAHSATKYIDGQGRVLGGLILG------KQELIKEVQF--- 231

Query: 244 AEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFASFLLLQGVETLSLRAERHGENA 303
                                      R  GP ++PF +++L + +ETL++R ERH  NA
Sbjct: 232 -------------------------FTRHTGPSISPFNAWILSRSMETLAIRMERHCANA 266

Query: 304 LKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNGFGGVLSFGVK-DLPNADKETDPF 362
           L +A + + +  + +V YP L SH  H+ AKK + +G GG+++  +K  +  A +     
Sbjct: 267 LAVASYFQDNKELEFVKYPFLKSHPQHDLAKKQMKHG-GGIVTLTLKGGIERAQR----- 320

Query: 363 KLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQLNDKEKLASGVTKDLIRVSVGIE 422
                  +D L++ +  AN+GD ++++  P  TTH +L ++E+   G+   LIR+S G+E
Sbjct: 321 ------FIDELQMITVTANLGDTRSIITHPASTTHSKLTEEERQRVGILPGLIRLSAGLE 374

Query: 423 FIDDIIADFQQSFE 436
             DDIIAD +++ E
Sbjct: 375 HHDDIIADIERALE 388


Lambda     K      H
   0.317    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 453
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 444
Length of database: 391
Length adjustment: 31
Effective length of query: 413
Effective length of database: 360
Effective search space:   148680
Effective search space used:   148680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory