Aligments for a candidate for cimA in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for Amino acid biosynthesis

Aligments for a candidate for cimA in Echinicola vietnamensis KMM 6221, DSM 17526

Align (R)-citramalate synthase CimA; EC 2.3.1.182 (characterized, see rationale)
to candidate Echvi_2061 Echvi_2061 Isopropylmalate/homocitrate/citramalate synthases

Query= uniprot:Q8F3Q1_LEPIN
         (516 letters)



>FitnessBrowser__Cola:Echvi_2061
          Length = 504

 Score =  528 bits (1361), Expect = e-154
 Identities = 266/503 (52%), Positives = 362/503 (71%), Gaps = 7/503 (1%)

Query: 11  LDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIMEWAA 70
           +D TLRDGEQT GVSF  SEKL IAK LL++L VDR+E+ASARVS+GELE V+KI  WAA
Sbjct: 1   MDTTLRDGEQTSGVSFLPSEKLQIAKLLLEELRVDRIEVASARVSEGELEGVKKITHWAA 60

Query: 71  TEQLTERIEILGFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTPKEFFTDVSFV 130
            +   + +E+LGFVD   +VDW+ ++GAKVLNLLTKGSL+HL  QL KTP E F  +   
Sbjct: 61  EKGYLDCVEVLGFVDTPASVDWLTEAGAKVLNLLTKGSLNHLTHQLKKTPVEHFAAIEKC 120

Query: 131 IEYAIKSGLKINVYLEDWSNGFRNSPDYVKSLVEHLSKEHIERIFLPDTLGVLSPEETFQ 190
           I YA + G+ +NVYLEDWS+G R+S DY   L+  L+ ++++R+ LPDTLG+L P E  +
Sbjct: 121 IHYANEKGISVNVYLEDWSSGMRHSRDYTLELIAFLADQNVKRVMLPDTLGLLKPAEVAE 180

Query: 191 GVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGERAGNTPLEALV 250
            V  + +++P++HF+FH HNDYDLSVAN ++AI  G+ G+H ++NGLGERAGN PLE++V
Sbjct: 181 YVGLVSEQFPEVHFDFHAHNDYDLSVANVMEAINHGISGIHTTVNGLGERAGNAPLESVV 240

Query: 251 TTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGN 310
            T+ D +  K N+ E  I   S+LVE FSG  I +N+P+VGE+VFTQTAG+HADGD K N
Sbjct: 241 ATLSDFTTVKLNVQENKIYRISKLVEQFSGLHIPSNKPVVGENVFTQTAGIHADGDNKKN 300

Query: 311 LYANPILPERFGRKRSYALGKLAGKASISENVKQLGMVLSEVVLQKVLERVIELGDQNKL 370
           LY N +LPERFGR R YALGK +GKA+I +N+ +LG+ L    L KV +++IELGD+ + 
Sbjct: 301 LYFNDLLPERFGRTRKYALGKTSGKANILKNLLELGIKLEPEELSKVTQKIIELGDRKER 360

Query: 371 VTPEDLPFIIADV-SGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIHKEISEGDGG 429
           VT EDLP+II+DV    + +K ++I+  ++    G++P  Q++L+++ + ++  + G+G 
Sbjct: 361 VTTEDLPYIISDVLQNNSIKKDISIEGYHMTHSKGLKPTVQLKLKFKDQFYEAHASGNGQ 420

Query: 430 YDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLEEDQTFKTM 489
           +D+FM AL KI   L   +PKL D+ V IPPGGKTDA VET ITW      +  +  KT 
Sbjct: 421 FDSFMLALQKIYKSLNKKLPKLTDFSVSIPPGGKTDAFVETVITW------DYGRIIKTK 474

Query: 490 GVHPDQTVAAVHATEKMLNQILQ 512
           G+  DQTVAA+ ATEKMLN I Q
Sbjct: 475 GLDSDQTVAAMMATEKMLNIIEQ 497


Lambda     K      H
   0.315    0.134    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 793
Number of extensions: 31
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 516
Length of database: 504
Length adjustment: 34
Effective length of query: 482
Effective length of database: 470
Effective search space:   226540
Effective search space used:   226540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis