GapMind for Amino acid biosynthesis

 

Aligments for a candidate for metB in Echinicola vietnamensis KMM 6221, DSM 17526

Align cystathionine gamma-synthase (EC 2.5.1.48) (characterized)
to candidate Echvi_1734 Echvi_1734 Cystathionine beta-lyases/cystathionine gamma-synthases

Query= BRENDA::Q1M0P5
         (380 letters)



>lcl|FitnessBrowser__Cola:Echvi_1734 Echvi_1734 Cystathionine
           beta-lyases/cystathionine gamma-synthases
          Length = 369

 Score =  310 bits (794), Expect = 4e-89
 Identities = 177/381 (46%), Positives = 236/381 (61%), Gaps = 15/381 (3%)

Query: 1   MHMQTKLIHGGISEDATTGAVSVPIYQTSTYRQDAIGHHKGYEYSRSGNPTRFALEELIA 60
           M  +T  IHGG  + A   AV  PI  ++T+      H +   YSRS NP R ALEEL+A
Sbjct: 1   MKFETLAIHGGEKKSAPHRAVVQPITLSTTFEH----HEESLIYSRSQNPNRMALEELLA 56

Query: 61  DLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIID 120
            LE G    AF+SG A   AVF  L  G H++   D+Y G  +   + L K+ L  T  D
Sbjct: 57  QLEKGSAAAAFSSGNAAGMAVFQALPLGSHIVAPSDMYHGLKKQLVE-LFKDKLEVTFTD 115

Query: 121 TSDLSQIKKAIKPNTKALYLETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFATPYYQN 180
            SD   ++KAI+PNTK L++ETPSNP+LKI+D+ +   +AK+  +  + DNTFATP +QN
Sbjct: 116 LSDPENLEKAIQPNTKLLWIETPSNPMLKISDIRRLTKMAKEDDIRVVCDNTFATPVFQN 175

Query: 181 PLLLGADIVVHSGTKYLGGHSDVVAG-LVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQ 239
           PL LGAD+V+HS TKY GGHSD++ G L+T  ++   ++I   Q   G VL P D +LL 
Sbjct: 176 PLELGADLVMHSATKYFGGHSDILGGALITKKSDEFWKQIVNVQQTGGAVLSPFDCYLLV 235

Query: 240 RGIKTLGLRMQAHQKNALCVAEFLEKHPKVERVYYPGLPTHPNYELAKKQMRGFSGMLSF 299
           R IKTL  RM+ H ++A  +A FL++HPKVERV+YPGL  HP +++AK QM GF G+LSF
Sbjct: 236 RSIKTLAYRMRGHAEHAGMIATFLDQHPKVERVFYPGLTAHPGHDVAKSQMTGFGGILSF 295

Query: 300 TLKNDSE-ATPFVESLKLFILGESLGGVESLVGVPAFMTHACIPKTQREAAGIRDGLVRL 358
            +K   E A   + SLK +    SLGGVESL+   A +     P T+         L+RL
Sbjct: 296 LVKGKPEDADKLISSLKYYTNATSLGGVESLIERRAAVEG---PDTKTP-----QNLIRL 347

Query: 359 SVGIEHEQDLLEDLEQAFAKI 379
           SVG+EH  DLLED+E+AF  I
Sbjct: 348 SVGLEHLDDLLEDMEKAFYSI 368


Lambda     K      H
   0.318    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 411
Number of extensions: 15
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 380
Length of database: 369
Length adjustment: 30
Effective length of query: 350
Effective length of database: 339
Effective search space:   118650
Effective search space used:   118650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory