GapMind for Amino acid biosynthesis

 

Aligments for a candidate for metB in Echinicola vietnamensis KMM 6221, DSM 17526

Align cystathionine γ-lyase (EC 2.5.1.48) (characterized)
to candidate Echvi_2283 Echvi_2283 Cystathionine beta-lyases/cystathionine gamma-synthases

Query= metacyc::HP0106-MONOMER
         (380 letters)



>lcl|FitnessBrowser__Cola:Echvi_2283 Echvi_2283 Cystathionine
           beta-lyases/cystathionine gamma-synthases
          Length = 391

 Score =  276 bits (705), Expect = 9e-79
 Identities = 150/367 (40%), Positives = 219/367 (59%), Gaps = 11/367 (2%)

Query: 22  SVPIYQTSTYRQDAIGRHK--------GYEYSRSGNPTRFALEELIADLEGGVKGFAFAS 73
           S PIY TS++  D+    +        G  YSR  NP    L E +  +EG   G A AS
Sbjct: 24  SAPIYMTSSFTFDSAEEARQMFAEEIPGNIYSRYANPNSSDLIEKVCAVEGTEDGIATAS 83

Query: 74  GLAGIH-AVFSLLQSGDHVLLGDDVYGGTFRLFNQVLVKNGLSCTIIDTSDISQIKKAIK 132
           G+A +  ++ SLL+ GDH+L    ++G T +L  +V  K G++ T  D SDI    K +K
Sbjct: 84  GMAAMFGSMASLLEQGDHILASRSLFGSTHQLLTRVFPKWGITSTYGDISDIENWDKLVK 143

Query: 133 PNTKALYLETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFATPYYQNPLLLGADIVAHS 192
           PNTK L++ETPSNP L++ DL      AK H L+ +VDN FATPY Q P   GADIVAHS
Sbjct: 144 PNTKMLFIETPSNPGLEVIDLEWIGKFAKAHNLILVVDNCFATPYLQQPAKWGADIVAHS 203

Query: 193 GTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQRGIKTLGLRMEAH 252
            TKY+ G   V+ GL+    E L +E+ FF    G  + P ++W+L R ++TL +RME H
Sbjct: 204 ATKYIDGQGRVLGGLILGKQE-LIKEVQFFTRHTGPSISPFNAWILSRSMETLAIRMERH 262

Query: 253 QKNALCVAEFLEKHPKVERVYYPGLPTHPNYELAKKQMRGFSGMLSFTLKNDSE-AVAFV 311
             NAL VA + + + ++E V YP L +HP ++LAKKQM+   G+++ TLK   E A  F+
Sbjct: 263 CANALAVASYFQDNKELEFVKYPFLKSHPQHDLAKKQMKHGGGIVTLTLKGGIERAQRFI 322

Query: 312 ESLKLFILGESLGGVESLVGIPAFMTHACIPKTQREAAGIRDGLVRLSVGIEHEQDLLED 371
           + L++  +  +LG   S++  PA  TH+ + + +R+  GI  GL+RLS G+EH  D++ D
Sbjct: 323 DELQMITVTANLGDTRSIITHPASTTHSKLTEEERQRVGILPGLIRLSAGLEHHDDIIAD 382

Query: 372 LEQAFAK 378
           +E+A  +
Sbjct: 383 IERALER 389


Lambda     K      H
   0.319    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 385
Number of extensions: 22
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 380
Length of database: 391
Length adjustment: 30
Effective length of query: 350
Effective length of database: 361
Effective search space:   126350
Effective search space used:   126350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory