Aligments for a candidate for argD in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Aligments for a candidate for argD in Cupriavidus basilensis 4G11

Align 4-aminobutyrate aminotransferase GabT; (S)-3-amino-2-methylpropionate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.22 (characterized)
to candidate RR42_RS26240 RR42_RS26240 4-aminobutyrate aminotransferase

Query= SwissProt::P22256
         (426 letters)



>FitnessBrowser__Cup4G11:RR42_RS26240
          Length = 420

 Score =  480 bits (1236), Expect = e-140
 Identities = 237/414 (57%), Positives = 307/414 (74%), Gaps = 2/414 (0%)

Query: 4   NKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYLDFAGGIAVLNTGHLHPKVV 63
           N +L++R+  A PRGVG +   +A+RAEN  +WDVEG  Y+DFA GIAVLNTGH HP++V
Sbjct: 3   NADLVRRKDAATPRGVGVMCNFYAERAENSELWDVEGNRYIDFAAGIAVLNTGHRHPRLV 62

Query: 64  AAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFAKKTLLVTTGSEAVENAVKIAR 123
            A++AQ+++ +HT +Q++ Y  Y+EL E +N + PG FAKKT   TTG+EAVENA+KIAR
Sbjct: 63  QAMQAQMERFTHTAYQIVPYASYIELAEKINARAPGAFAKKTAFFTTGAEAVENAIKIAR 122

Query: 124 AATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMGLMPGHVYRALYPCPLHGISEDDA 183
           AAT R G IAFSG +HGRT   +ALTGKV PY  G G  PG V+ A YP  LHG+S  D+
Sbjct: 123 AATGRPGVIAFSGGFHGRTMMGMALTGKVVPYKVGFGPFPGDVFHAPYPYGLHGVSVQDS 182

Query: 184 IASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASSPAFMQRLRALCDEHGIMLIADEVQS 243
           I ++H++FK D  P+ +AAI+ EPVQGEGGF  +   F++ LRA+CDEHGI+L+ADEVQ+
Sbjct: 183 INALHQLFKADVDPKRVAAIIFEPVQGEGGFNVAPAEFVRALRAICDEHGILLVADEVQT 242

Query: 244 GAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPLAGVTGRAEVMDAVAPGGLGGTYAGNPI 303
           G GRTG LFAME   V PDLTT AKS+AGG PL+ V GRAE+MDA APGGLGGTYAGNP+
Sbjct: 243 GFGRTGKLFAMEHYDVTPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGNPL 302

Query: 304 ACVAALEVLKVFEQENLLQKANDLGQKLKDGLLAIAEKHPEIGDVRGLGAMIAIELFEDG 363
           A  +AL VL V E E L+++   LGQ+L+D L  +  + PEIG+VRG+GAMIA+E F   
Sbjct: 303 AVASALAVLDVLESEKLIERGAALGQRLQDKLDGLKSRVPEIGEVRGVGAMIAVE-FRKA 361

Query: 364 DHNKPDAKLTAEIVARARDKGLILLSCGPYYNVLRILVPLTIEDAQIRQGLEII 417
           D  +PD + T ++  RA ++GL+LLSCG Y NV+R L PLTI DA + +GL I+
Sbjct: 362 D-GRPDPEFTRQVQDRALERGLLLLSCGVYGNVVRFLFPLTIPDAVMDEGLGIL 414


Lambda     K      H
   0.320    0.137    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 593
Number of extensions: 25
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 426
Length of database: 420
Length adjustment: 32
Effective length of query: 394
Effective length of database: 388
Effective search space:   152872
Effective search space used:   152872
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis