Alignments for a candidate for gatA in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Cupriavidus basilensis 4G11

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate RR42_RS26835 RR42_RS26835 amidase

Query= curated2:Q67KJ2
         (488 letters)



>FitnessBrowser__Cup4G11:RR42_RS26835
          Length = 460

 Score =  211 bits (536), Expect = 6e-59
 Identities = 157/471 (33%), Positives = 235/471 (49%), Gaps = 44/471 (9%)

Query: 3   SAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKAGD 62
           S A +     +G LSA ++  ++L+R AQV  ++     + A   +  A   D  R AG 
Sbjct: 7   SVAGIAAQLRSGSLSAQDVVRASLARAAQVA-SLNCVSRLLAREAVVGAGACDTARLAGK 65

Query: 63  TELGPLAGVPIAVKDNICTSGMETTCASRILKGYVSPF--DATVVERLRAAGAMIIGKAN 120
             L PLAGVP  VK+ +   G   T A    +    P    A VV+ L AAGA+ +    
Sbjct: 66  APL-PLAGVPFVVKNLLDVEG-HATLAGAHARANEPPATRSAAVVQALMAAGAIPVALTQ 123

Query: 121 MDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVAAGEAPLALGTDTGGSIRQPA 180
           MDE+A G++GE+   G  RNP D  R+ GGSS+G+AAA+AAG AP+ +G+DT GSIR PA
Sbjct: 124 MDEYACGATGENVIGGPVRNPLDPARIAGGSSAGTAAAIAAGIAPIGIGSDTNGSIRAPA 183

Query: 181 AFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGR 240
           +F GI GL+PT G +S  G   +A SLD VGPM  D   +A  +  + GP        G 
Sbjct: 184 SFCGIWGLRPTTGRMSAQGCFPYAESLDAVGPMASDAAGLALAYRAMLGPG--SGPRPGE 241

Query: 241 TPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTE 300
             PA       S++G+R+G+    L  G     +   ++A+ ++      V +  LP  +
Sbjct: 242 PAPA----AGVSMAGLRVGI----LQAGFGDFAQDEAQDAVLRVASAFDAVRKIDLPEAD 293

Query: 301 YALSAYYVIAVAEASSNLARFDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGT 360
            A  A  +I+  E   N      V+ G+               R  G+   V++R++ G 
Sbjct: 294 LARDAASIISAFEVGRN-----HVQRGF-------------AERHPGYSAFVRQRMLAGL 335

Query: 361 YVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPTTPFTAWKIG-EKVDDPVSMY 419
            +      + +Y+ A+  +    +  E  F+  D L+  TTP+ A  IG E++      Y
Sbjct: 336 SI-----PEGWYQLAKAYQAAWRQKLEALFDEVDLLLACTTPYAAPLIGAEQIAGGKRAY 390

Query: 420 L----GDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIA 466
           +        T PV+LAGLP V+ PC  V GLP+G+QLI  P+ +T+ L+ A
Sbjct: 391 VPRADAGHLTRPVSLAGLPVVAAPCA-VAGLPLGVQLIAPPWQETRCLEAA 440


Lambda     K      H
   0.318    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 581
Number of extensions: 37
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 488
Length of database: 460
Length adjustment: 33
Effective length of query: 455
Effective length of database: 427
Effective search space:   194285
Effective search space used:   194285
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis