Align Serine O-succinyltransferase; SST; Homoserine O-succinyltransferase; HST; Homoserine transsuccinylase; HTS; EC 2.3.1.-; EC 2.3.1.46 (characterized)
to candidate RR42_RS01160 RR42_RS01160 homoserine O-acetyltransferase
Query= SwissProt::S2KHP1 (367 letters) >FitnessBrowser__Cup4G11:RR42_RS01160 Length = 390 Score = 268 bits (684), Expect = 2e-76 Identities = 155/357 (43%), Positives = 206/357 (57%), Gaps = 9/357 (2%) Query: 2 PDARRFIELPGPVRMYRGGELPSVTIAYETWGELRGQGDNALLLFTGLSPSAHAASSMA- 60 P F E P+++ G L + ET+G L NA+L+ L+ S H A Sbjct: 23 PQCMHFTE---PLKLRNGTSLADYDLMVETYGTLNAARSNAVLVCHALNASHHVAGVYEH 79 Query: 61 DPSP-GWWEYMIGPGKPIDTERFFVIAINSLGSCFGSTGPASINPATGQPYRLDFPKLSV 119 DP GWW+ M+GPGKP+DT+RFFVI +N+LGSCFGSTGP S NPATGQPY FP ++V Sbjct: 80 DPRDVGWWDNMVGPGKPLDTDRFFVIGVNNLGSCFGSTGPMSANPATGQPYGATFPVVTV 139 Query: 120 EDIVAAARGACRALGIDHVHTVAGASLGGMDALAYAVMYPGTYRDIISISAAAHATPFTI 179 ED V A GI V G SLGGM ALA+++MYP R I +++ + I Sbjct: 140 EDWVNAQARVADRFGITQFAAVMGGSLGGMQALAWSLMYPDRLRHCIVVASTPKLSAQNI 199 Query: 180 ALRSIQREAVRADPAWAGGN-YAPGEGPKDGMRVARQLGILTYRSAEEWLQRFDRERLEG 238 A + R A+ +DP + GGN YA G PK G+RVAR +G +TY S E+ ++F RE Sbjct: 200 AFNEVARSAILSDPDFHGGNYYAHGVKPKRGLRVARMIGHITYLSDEDMAEKFGREL--K 257 Query: 239 SDDSANPFAMAFQVQSYMEANARKFADRFDANCYLYLSQAMDLFDMAEHGDGSLEAAVRR 298 +DD F + FQV+SY+ KFA+ FDAN YL +++A+D FD A G L AV + Sbjct: 258 TDDIRFSFDVEFQVESYLRYQGDKFAEYFDANTYLLITRALDYFDPALAFGGDLTRAVSQ 317 Query: 299 IDAKRALVAGVTTDWLFPLWQQRQVAELLEHAGVAVSYHELGSIQGHDAFLVDSERF 355 A LVA TTDW F + R++ + L VSY E+ + GHDAFL+D R+ Sbjct: 318 TQAS-FLVASFTTDWRFAPNRSRELVKALLDNKRPVSYAEIDAPHGHDAFLLDDPRY 373 Lambda K H 0.320 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 395 Number of extensions: 15 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 367 Length of database: 390 Length adjustment: 30 Effective length of query: 337 Effective length of database: 360 Effective search space: 121320 Effective search space used: 121320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory