Alignments for a candidate for agx1 in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for agx1 in Cupriavidus basilensis 4G11

Align Alanine--glyoxylate aminotransferase 2 homolog 1, mitochondrial; Beta-alanine-pyruvate aminotransferase 1; EC 2.6.1.44 (characterized)
to candidate RR42_RS21370 RR42_RS21370 4-aminobutyrate aminotransferase

Query= SwissProt::Q940M2
         (476 letters)



>FitnessBrowser__Cup4G11:RR42_RS21370
          Length = 420

 Score =  179 bits (453), Expect = 2e-49
 Identities = 131/396 (33%), Positives = 196/396 (49%), Gaps = 26/396 (6%)

Query: 89  LYDESGRRYLDAFAGIVTVSCGHCHPDILNAITEQSKLLQH-ATTIYLHHAIGDFAEALA 147
           ++D  G+RY+D  AGI  ++ GH HP ++ A+  Q +   H A  I  + +  + AE + 
Sbjct: 34  IWDVEGKRYIDFAAGIAVLNTGHRHPRLVEAMQRQLERFTHTAYQIVPYASYIELAERIN 93

Query: 148 AKMPGN-LKVVYFVNSGSEANELAMMMARLYTGSLEMISLRNAYHGGSSNTIGLTA-LNT 205
            + PG   K   F  +G+EA E A+ +AR  TG   +I+    +HG +   + LT  +  
Sbjct: 94  QRAPGRGAKKTAFFTTGAEAVENAIKIARAATGRPGVIAFSGGFHGRTMMGMALTGKVVP 153

Query: 206 WKY---PLPQGEIHHVVNPDPYRGVFGSDG-----SLYAKDVHDHIEYGTSGKVAGFIAE 257
           +K    P P GE+ H   P    GV   D       L+  DV          +VA  I E
Sbjct: 154 YKVGFGPFP-GEVFHAPYPCALHGVSVEDALKAMEHLFKADVDPK-------RVAAIIFE 205

Query: 258 TIQGVGGAVELAPGYLKSVYEIVRNAGGVCIADEVQTGFGRTGSHYWGFQTQDVVPDIVT 317
            +QG GG     P ++K++  I    G + +ADEVQTGFGRTG  +   +  DV PD+ T
Sbjct: 206 PVQGEGGFNVAPPAFVKALRAICDEHGILLVADEVQTGFGRTGKLF-AMEHYDVAPDLTT 264

Query: 318 MAKGIGNGLPLGAVVTTPEIASVLASKILFNTFGGNPVCSAGGLAVLNVIDKEKRQEHCA 377
           MAK +  G+PL AV    E+    A   L  T+ GNP+  A  LAVL+V++ E+     A
Sbjct: 265 MAKSLAGGMPLSAVCGRAEVMDAPAPGGLGGTYAGNPLAVASALAVLDVLEGEQLIPRGA 324

Query: 378 EVGSHLIQRLKDVQKRHDIIGDVRGRGLMVGIELVSDRKDKTPAKAETSVLFEQLRELGI 437
            +G  L+ RL+ ++ R   I ++RG G MV +E    R D  P    T  +  +  E G+
Sbjct: 325 ALGQRLVARLEALKARVPQIAEIRGLGAMVAVEF--RRADGAPDAEFTREVQNRALEQGL 382

Query: 438 LVGKGGLHGNVFRIKPPMCFT----KDDADFLVDAL 469
           L+   G++GNV R   P+  +     +  D L D L
Sbjct: 383 LLLSCGVYGNVIRFLFPLTISDAVMNEGLDILADVL 418


Lambda     K      H
   0.320    0.138    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 528
Number of extensions: 36
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 476
Length of database: 420
Length adjustment: 33
Effective length of query: 443
Effective length of database: 387
Effective search space:   171441
Effective search space used:   171441
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis