GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapC in Cupriavidus basilensis 4G11

Align acetylornithine/N-succinyldiaminopimelate aminotransferase [EC:2.6.1.11 2.6.1.17] (characterized)
to candidate RR42_RS16955 RR42_RS16955 acetylornithine aminotransferase

Query= reanno::azobra:AZOBR_RS19025
         (389 letters)



>lcl|FitnessBrowser__Cup4G11:RR42_RS16955 RR42_RS16955
           acetylornithine aminotransferase
          Length = 395

 Score =  281 bits (718), Expect = 3e-80
 Identities = 158/382 (41%), Positives = 228/382 (59%), Gaps = 8/382 (2%)

Query: 11  RADIVFERGEGPYLYATDGRRFLDFAAGVAVNVLGHANPYLVEALTAQAHKLWHTSNLFR 70
           R ++VF  G+G +L   +G+R+LDF  G AVN LGH+N  +++AL  Q+ KL++ S  F 
Sbjct: 17  RPELVFTEGKGSWLTDHNGKRYLDFVQGWAVNCLGHSNQAMIDALVDQSKKLFNPSPAFY 76

Query: 71  VAGQESLAKRLTEATFADTVFFTNSGAEAWECGAKLIRKYHYEKGDKARTRIITFEQAFH 130
                 LA++LT+A+  D VFF NSGAEA E   KL RK+   K       IIT + +FH
Sbjct: 77  NEPMLRLARQLTDASCFDKVFFANSGAEANEGAIKLARKWG-RKHKNGAFEIITMDHSFH 135

Query: 131 GRTLAAVSAAQQEKLIKGFGPLLDGFDLVPFGDLEAVRNAVTDETAGICLEPIQGEGGIR 190
           GRTLA +SA+ +      F P + GF      DL +V   + D+T  I LEP+QGEGG+ 
Sbjct: 136 GRTLATMSASGKAGWDTIFAPQVPGFPKADLNDLASVEKLINDKTVAIMLEPVQGEGGVI 195

Query: 191 AGSVEFLRGLREICDEHGLLLFLDEIQCGMGRTGKLFAHEWAGITPDVMAVAKGIGGGFP 250
             S EF++GLR++ D+H LL  +DE+Q G GR G +FA+E +G+ PD+M + KGIGGG P
Sbjct: 196 PASREFMQGLRKLADQHKLLFIVDEVQTGCGRCGTMFAYELSGVEPDIMTLGKGIGGGVP 255

Query: 251 LGACLATEKAASGMTAGTHGSTYGGNPLATAVGNAVLDKVLEPGFLDHVQRIGGLLQDRL 310
           L A L   + AS   AG  G TY GNP+ TAVG+AV+ ++  PGFL  VQ  G  L+++L
Sbjct: 256 LAALLCKAEVAS-FEAGDQGGTYNGNPVMTAVGSAVISQLTAPGFLQSVQDKGAYLREQL 314

Query: 311 AGLVAENPAVFKGVRGKGLMLGLACGPAVGDVVVA----LRANGLLSVPAGDNVVRLLPP 366
             L +E      G RG+GL+  L     +G  +V     ++  GLL      N++R +P 
Sbjct: 315 LALTSE--FGLGGERGEGLLRALVLNKDIGPQLVEEARDMQPQGLLLNSPRPNLLRFMPA 372

Query: 367 LNIGEAEVEEAVAILAKTAKEL 388
           LN+   E+++ +++L    K+L
Sbjct: 373 LNVTIEEIDQMISMLRTLLKKL 394


Lambda     K      H
   0.321    0.139    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 382
Number of extensions: 19
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 389
Length of database: 395
Length adjustment: 31
Effective length of query: 358
Effective length of database: 364
Effective search space:   130312
Effective search space used:   130312
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory