Alignments for a candidate for hcs in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for hcs in Cupriavidus basilensis 4G11

Align homocitrate synthase (EC 2.3.3.14) (characterized)
to candidate RR42_RS05465 RR42_RS05465 2-isopropylmalate synthase

Query= BRENDA::D0VY45
         (540 letters)



>FitnessBrowser__Cup4G11:RR42_RS05465
          Length = 512

 Score =  402 bits (1032), Expect = e-116
 Identities = 237/512 (46%), Positives = 320/512 (62%), Gaps = 20/512 (3%)

Query: 27  ILDTTLRDGEQSPGAAMTCVQKLETARQLAKLGVDIIEAGFPCASKQDFMAVKMIAEEVG 86
           I DTTLRDGEQSPGA+MT  +K+  ARQL +L VD+IEAGF  +S  DF A++ IA+ V 
Sbjct: 7   IFDTTLRDGEQSPGASMTREEKIRIARQLERLKVDVIEAGFAASSNGDFEAIRSIAQVVK 66

Query: 87  NCVDGNGYVPVITGVSRCNEKDIATAWEALKHAKRPRLRTFIATSPIHMEYKLRKSKDQV 146
           +          I  ++R N++DIA A EALK A   R+ TFIATS +HME KLR + DQV
Sbjct: 67  DST--------ICSLARANDRDIARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQV 118

Query: 147 LETARNMVKFARSLGCTDIQFGAEDAARSDKEFLYQIFGEVIKAGATTLTIPDTVGIAMP 206
            E A   V+FAR     DI+F  ED +RSD +FL ++   VIK GATT+ +PDTVG A+P
Sbjct: 119 FEQAIQAVRFARQF-TDDIEFSPEDGSRSDMDFLCRVLEGVIKEGATTINLPDTVGYAVP 177

Query: 207 FEYGKLIADIKANTPGIENAIMATHCHNDLGLATANTIEGARY-GARQLEVTINGIGERA 265
             Y  LI  ++   P  + AI + HCHNDLG+A AN++   +  GARQ+E TING+GERA
Sbjct: 178 EGYAGLIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQVECTINGLGERA 237

Query: 266 GNASFEEVVMALTCRGIDILGGLHTGINTRHILKTSKMVEKYSGLHLQPHKALVGANAFL 325
           GN S EEVVMA+  R       +  G++T  I+  SK+V + +G  +QP+KA+VGANAF 
Sbjct: 238 GNTSLEEVVMAVKTRRDYF--DMDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFA 295

Query: 326 HESGIHQDGMLKHRGTYEIISPEDIGLVRSVGDTIVLGKLSGRQALRNRLEELGYKLK-D 384
           H SGIHQDG+LK R TYEI+  ED+G      + IVLGKLSGR A + RL+ELG +L+ +
Sbjct: 296 HASGIHQDGVLKARDTYEIMRAEDVGW---SANKIVLGKLSGRNAFKQRLQELGIELESE 352

Query: 385 TEVEGVFWQFKAVAEKKKRITDTDLRALVSNEAFNEQPIWKLGDLQVTCGTVGFSTATVK 444
            EV   F +FK +A++K  I D D+ A+VS+EA +    ++   L     T     A V 
Sbjct: 353 GEVNAAFTRFKELADQKSEIFDEDIMAIVSDEAHDVNEHFRFISLSQHSETGERPHARV- 411

Query: 445 LFSIDGSMHVACSIGTGPVDSAYKAINHIVKEPAKLVKYTLGAITEGIDATATTSVEISR 504
           +F++DG+       G GPVD+   AI   V   A+LV Y++ AIT G  A    +V +S+
Sbjct: 412 VFNMDGNEVTGEGEGNGPVDATLHAIEGKVASGAELVLYSVNAITAGTQAQGEVTVRLSK 471

Query: 505 GDTNHPVFSGTGGGTDVVVSSVDAYLSALNNM 536
           G     + +G G   D+V +S  AYL+ALN +
Sbjct: 472 GGR---IVNGVGTDPDIVAASAKAYLAALNKL 500


Lambda     K      H
   0.318    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 600
Number of extensions: 30
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 540
Length of database: 512
Length adjustment: 35
Effective length of query: 505
Effective length of database: 477
Effective search space:   240885
Effective search space used:   240885
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis