Alignments for a candidate for lysN in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysN in Cupriavidus basilensis 4G11

Align 2-aminoadipate transaminase (2.6.1.39) (characterized)
to candidate RR42_RS21370 RR42_RS21370 4-aminobutyrate aminotransferase

Query= reanno::Putida:PP_4108
         (416 letters)



>FitnessBrowser__Cup4G11:RR42_RS21370
          Length = 420

 Score =  375 bits (963), Expect = e-108
 Identities = 197/390 (50%), Positives = 249/390 (63%), Gaps = 2/390 (0%)

Query: 23  NAEVWDTDGKRYIDFVGGIGVLNLGHCNPAVVEAIQAQATRLTHYAFNAAPHGPYLALME 82
           N+E+WD +GKRYIDF  GI VLN GH +P +VEA+Q Q  R TH A+   P+  Y+ L E
Sbjct: 31  NSEIWDVEGKRYIDFAAGIAVLNTGHRHPRLVEAMQRQLERFTHTAYQIVPYASYIELAE 90

Query: 83  QLSQFVPVSYPLAGMLTNSGAEAAENALKVARGATGKRAIIAFDGGFHGRTLATLNLNGK 142
           +++Q  P           +GAEA ENA+K+AR ATG+  +IAF GGFHGRT+  + L GK
Sbjct: 91  RINQRAPGRGAKKTAFFTTGAEAVENAIKIARAATGRPGVIAFSGGFHGRTMMGMALTGK 150

Query: 143 VAPYKQRVGELPGPVYHLPYPSADTGVTCEQALKAMDRLFSVELAVEDVAAFIFEPVQGE 202
           V PYK   G  PG V+H PYP A  GV+ E ALKAM+ LF  ++  + VAA IFEPVQGE
Sbjct: 151 VVPYKVGFGPFPGEVFHAPYPCALHGVSVEDALKAMEHLFKADVDPKRVAAIIFEPVQGE 210

Query: 203 GGFLALDPAFAQALRRFCDERGILIIIDEIQSGFGRTGQRFAFPRLGIEPDLLLLAKSIA 262
           GGF    PAF +ALR  CDE GIL++ DE+Q+GFGRTG+ FA     + PDL  +AKS+A
Sbjct: 211 GGFNVAPPAFVKALRAICDEHGILLVADEVQTGFGRTGKLFAMEHYDVAPDLTTMAKSLA 270

Query: 263 GGMPLGAVVGRKELMAALPKGGLGGTYSGNPISCAAALASLAQMTDENLATWGERQEQAI 322
           GGMPL AV GR E+M A   GGLGGTY+GNP++ A+ALA L  +  E L   G    Q +
Sbjct: 271 GGMPLSAVCGRAEVMDAPAPGGLGGTYAGNPLAVASALAVLDVLEGEQLIPRGAALGQRL 330

Query: 323 VSRYERWKASGLSPYIGRLTGVGAMRGIEFANADGSPAPAQLAKVMEAARARGLLLMPSG 382
           V+R E  KA    P I  + G+GAM  +EF  ADG+P      +V   A  +GLLL+  G
Sbjct: 331 VARLEALKAR--VPQIAEIRGLGAMVAVEFRRADGAPDAEFTREVQNRALEQGLLLLSCG 388

Query: 383 KARHIIRLLAPLTIEAEVLEEGLDILEQCL 412
              ++IR L PLTI   V+ EGLDIL   L
Sbjct: 389 VYGNVIRFLFPLTISDAVMNEGLDILADVL 418


Lambda     K      H
   0.320    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 547
Number of extensions: 22
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 416
Length of database: 420
Length adjustment: 32
Effective length of query: 384
Effective length of database: 388
Effective search space:   148992
Effective search space used:   148992
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis