Alignments for a candidate for PAH in Cupriavidus basilensis 4G11

GapMind for Amino acid biosynthesis

Alignments for a candidate for PAH in Cupriavidus basilensis 4G11

Align phenylalanine 4-monooxygenase (EC 1.14.16.1) (characterized)
to candidate RR42_RS20365 RR42_RS20365 phenylalanine-4-hydroxylase

Query= BRENDA::P30967
         (297 letters)



>FitnessBrowser__Cup4G11:RR42_RS20365
          Length = 310

 Score =  426 bits (1095), Expect = e-124
 Identities = 198/272 (72%), Positives = 228/272 (83%)

Query: 26  DFTLPQPLDRYSAEDHATWATLYQRQCKLLPGRACDEFMEGLERLEVDADRVPDFNKLNQ 85
           DFT+ QPL+RY+  DHA WA LY RQ  +L GR CDEF++GL  L ++ DRVP F++LN+
Sbjct: 39  DFTIAQPLERYTDTDHAVWAKLYDRQASMLRGRVCDEFLQGLSTLGMERDRVPSFDQLNE 98

Query: 86  KLMAATGWKIVAVPGLIPDDVFFEHLANRRFPVTWWLREPHQLDYLQEPDVFHDLFGHVP 145
            LM ATGW++VAVPGL+PD VFFEHLANRRFP +WW+R+P QLDYLQEPD FHD+FGHVP
Sbjct: 99  TLMRATGWQVVAVPGLVPDAVFFEHLANRRFPASWWMRKPEQLDYLQEPDCFHDVFGHVP 158

Query: 146 LLINPVFADYLEAYGKGGVKAKALGALPMLARLYWYTVEFGLINTPAGMRIYGAGILSSK 205
           LLINPVFA+Y+EAYGKGG+KA +LGAL MLARLYWYTVEFGLI TP G+RIYGAGILSS+
Sbjct: 159 LLINPVFANYMEAYGKGGLKAASLGALDMLARLYWYTVEFGLIRTPEGLRIYGAGILSSQ 218

Query: 206 SESIYCLDSASPNRVGFDLMRIMNTRYRIDTFQKTYFVIDSFKQLFDATAPDFAPLYLQL 265
            ESIY LDSASPNR+GFD+ RIM TRYRIDTFQKTYFVIDSF QLFDAT PDFAPLY +L
Sbjct: 219 GESIYSLDSASPNRIGFDVRRIMRTRYRIDTFQKTYFVIDSFDQLFDATRPDFAPLYEEL 278

Query: 266 ADAQPWGAGDVAPDDLVLNAGDRQGWADTEDV 297
                 GAGDV   DLVL  G R+GWAD++DV
Sbjct: 279 RAQPTLGAGDVGQSDLVLQRGSREGWADSDDV 310


Lambda     K      H
   0.323    0.140    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 372
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 297
Length of database: 310
Length adjustment: 27
Effective length of query: 270
Effective length of database: 283
Effective search space:    76410
Effective search space used:    76410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate RR42_RS20365 RR42_RS20365 (phenylalanine-4-hydroxylase)
to HMM TIGR01267 (phhA: phenylalanine-4-hydroxylase (EC 1.14.16.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01267.hmm
# target sequence database:        /tmp/gapView.24950.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01267  [M=248]
Accession:   TIGR01267
Description: Phe4hydrox_mono: phenylalanine-4-hydroxylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.3e-123  395.7   0.0   4.1e-123  395.4   0.0    1.1  1  lcl|FitnessBrowser__Cup4G11:RR42_RS20365  RR42_RS20365 phenylalanine-4-hyd


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Cup4G11:RR42_RS20365  RR42_RS20365 phenylalanine-4-hydroxylase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  395.4   0.0  4.1e-123  4.1e-123       1     244 [.      39     283 ..      39     287 .. 0.98

  Alignments for each domain:
  == domain 1  score: 395.4 bits;  conditional E-value: 4.1e-123
                                 TIGR01267   1 dftvaqdldryseeehavwatlidrqlkllegracdeyldGveklgldadripdleevneklraltGwk 69 
                                               dft+aq+l+ry++++havwa l+drq+ +l+gr cde+l+G+  lg++ dr+p ++++ne l+++tGw+
  lcl|FitnessBrowser__Cup4G11:RR42_RS20365  39 DFTIAQPLERYTDTDHAVWAKLYDRQASMLRGRVCDEFLQGLSTLGMERDRVPSFDQLNETLMRATGWQ 107
                                               79******************************************************************* PP

                                 TIGR01267  70 ivavpglipadvffehlanrrfpvttflrtpeeldylqepdvfhdlfGhvpllsnpvfadfleayGkkg 138
                                               +vavpgl+p++vffehlanrrfp ++++r+pe+ldylqepd fhd+fGhvpll+npvfa+++eayGk+g
  lcl|FitnessBrowser__Cup4G11:RR42_RS20365 108 VVAVPGLVPDAVFFEHLANRRFPASWWMRKPEQLDYLQEPDCFHDVFGHVPLLINPVFANYMEAYGKGG 176
                                               ********************************************************************* PP

                                 TIGR01267 139 vkakalgaa.llarlywytvefGlvetaaglriyGaGilsskkelvyaleskeplrvafdllevmrtry 206
                                               +ka  lga  +larlywytvefGl++t++glriyGaGilss+ e++y+l+s++p+r++fd+ ++mrtry
  lcl|FitnessBrowser__Cup4G11:RR42_RS20365 177 LKAASLGALdMLARLYWYTVEFGLIRTPEGLRIYGAGILSSQGESIYSLDSASPNRIGFDVRRIMRTRY 245
                                               *******977*********************************************************** PP

                                 TIGR01267 207 ridklqkayfvlpslkrlfdaaqedfealvaeakdlka 244
                                               rid++qk+yfv++s+++lfda+++df++l+ e++ + +
  lcl|FitnessBrowser__Cup4G11:RR42_RS20365 246 RIDTFQKTYFVIDSFDQLFDATRPDFAPLYEELRAQPT 283
                                               ******************************99887655 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (248 nodes)
Target sequences:                          1  (310 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 8.10
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis