Align (R)-citramalate synthase (EC 2.3.3.21) (characterized)
to candidate 3608293 Dshi_1696 2-isopropylmalate synthase/homocitrate synthase family protein (RefSeq)
Query= reanno::DvH:207383 (538 letters) >FitnessBrowser__Dino:3608293 Length = 545 Score = 414 bits (1065), Expect = e-120 Identities = 240/544 (44%), Positives = 335/544 (61%), Gaps = 24/544 (4%) Query: 1 MRRQIHLYDTTLRDGSQSEDINLNTPDKLKIALRLDELGIAYIEGGWPGSNPVDVAFFKE 60 M +++LYDTTLRDG Q++ + +TP+K +IA LD LGI +IEGGWPG+NP D FF Sbjct: 1 MNERLYLYDTTLRDGQQTQGVQFSTPEKQQIAAALDALGIDFIEGGWPGANPTDSEFFAS 60 Query: 61 IRNYNLKQAKISAFGSTHHPSHTAENDPNLKAIAAARTDAAAIFGKSCERHAREALRLDG 120 ++ A+ +AFG T +AEND L A+ A T A + GKS E H AL + Sbjct: 61 APTFD--HARFAAFGMTKRAGFSAENDDVLAAVLNAGTQAVCLVGKSHEYHVTRALGISL 118 Query: 121 RRNLDIIHDSIAFLKKQVAEVFFDAEHFFDGYRHNAAYALEVLRRAHEAGGDVLVLCDTN 180 N + I S+A L Q E FDAEHFFDGY N YA+E L+ A EAG +VLCDTN Sbjct: 119 AENTENIARSVAHLVAQGREAIFDAEHFFDGYLANPGYAVETLQAALEAGARWVVLCDTN 178 Query: 181 GGTLPHEVHDIVT-AVREQLPEAKLGIHAHNDCEVAVANSIAAVQAGAVQVQGTMNGVGE 239 GG LP EV I + +P +LGIH HND AVAN++AA+ AGA QVQGT+NG+GE Sbjct: 179 GGRLPAEVGRITAEVIAAGVPGDRLGIHTHNDTGNAVANALAAIDAGARQVQGTLNGLGE 238 Query: 240 RCGNANLSSVIPILELKSAGA---YACLPEGRLQQLTAVSSYVSEVTNLPPFSRQPFVGR 296 RCGNANL ++IP L LK A + L+ L VS + ++ N P P+VG Sbjct: 239 RCGNANLVTLIPTLLLKEPYASRFETGVSAAALRDLVRVSRTLDDILNRVPTKSAPYVGA 298 Query: 297 SAFAHKGGVHVSAVNRNATLYEHITPESVGNHQRVLITELAGRSNIVSLARRFGFHLDKD 356 SAFAHK G+H SA+ ++ + YEHI PE+VGN + + ++ AG+SN+ G + K Sbjct: 299 SAFAHKAGLHASAILKDPSTYEHIPPEAVGNARVIPMSNQAGQSNLRKRLADAGIAVQKG 358 Query: 357 EPVVKGLMNELKKKASLGYDYAAAEASVELLLLRKLARRGVREFFKLIQFRVLESKQEND 416 + + L++E+K++ +GY + +A+AS ELL R L G+ EFF++ ++RV +++N Sbjct: 359 DMRLGALLDEVKRREDMGYSFDSAQASFELLARRHL--EGLPEFFEVKRYRVTVERRKNK 416 Query: 417 LEPM---SEASVMVEVE-----GIIEHTAATG--RGPVNALDNALRKALSSFYPRIREMR 466 + M SEA V++++ + E TG RGPVNAL AL K L + P I ++R Sbjct: 417 YDRMVSLSEAVVVLKIGDEKMLSVSESMDETGCDRGPVNALAKALGKDLGPYTPYIADLR 476 Query: 467 LLDFKVRVLTGTETDGGTASTVRVLIESGDADS-RWVTVGVSYNIIEASWQALADSMTYK 525 L+DFKVR+ T+GGT + RVLI+S D RW TVGVS NI++AS++AL +++ +K Sbjct: 477 LVDFKVRI-----TNGGTEAVTRVLIDSEDGQGRRWSTVGVSANIVDASFEALVEAIQWK 531 Query: 526 LYKD 529 L +D Sbjct: 532 LIRD 535 Lambda K H 0.318 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 649 Number of extensions: 28 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 538 Length of database: 545 Length adjustment: 35 Effective length of query: 503 Effective length of database: 510 Effective search space: 256530 Effective search space used: 256530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate 3608293 Dshi_1696 (2-isopropylmalate synthase/homocitrate synthase family protein (RefSeq))
to HMM TIGR00977 (cimA: citramalate synthase (EC 2.3.1.182))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00977.hmm # target sequence database: /tmp/gapView.10537.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00977 [M=526] Accession: TIGR00977 Description: citramal_synth: citramalate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.7e-177 574.8 0.0 8.2e-177 574.5 0.0 1.0 1 lcl|FitnessBrowser__Dino:3608293 Dshi_1696 2-isopropylmalate synt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3608293 Dshi_1696 2-isopropylmalate synthase/homocitrate synthase family protein (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 574.5 0.0 8.2e-177 8.2e-177 2 520 .. 5 535 .. 4 540 .. 0.95 Alignments for each domain: == domain 1 score: 574.5 bits; conditional E-value: 8.2e-177 TIGR00977 2 lklydttlrdGaqaeGvslsledkiriaeklddlGihyieGGwpganpkdvaffekvkeenlknakvvafsstrrpd 78 l+lydttlrdG q++Gv +s +k +ia +ld lGi++ieGGwpganp d +ff + +++a+ +af+ t+r lcl|FitnessBrowser__Dino:3608293 5 LYLYDTTLRDGQQTQGVQFSTPEKQQIAAALDALGIDFIEGGWPGANPTDSEFFASA--PTFDHARFAAFGMTKRAG 79 89****************************************************876..5799************** PP TIGR00977 79 kkveedkqlqalikaetpvvtifGkswdlhveealkttleenlkmiydtveylkrfadeviydaehffdGykanpey 155 e+d l a+++a+t+ v + Gks + hv+ al +l+en + i +v++l +++e i+daehffdGy anp y lcl|FitnessBrowser__Dino:3608293 80 FSAENDDVLAAVLNAGTQAVCLVGKSHEYHVTRALGISLAENTENIARSVAHLVAQGREAIFDAEHFFDGYLANPGY 156 ***************************************************************************** PP TIGR00977 156 alktlkvaekaGadwlvladtnGGtlpheieeitkkvkk.rlkdpqlGihahndsetavansllaveaGavqvqGti 231 a++tl++a +aGa w+vl+dtnGG lp e+ it +v + +lGih+hnd++ avan+l+a+ aGa+qvqGt+ lcl|FitnessBrowser__Dino:3608293 157 AVETLQAALEAGARWVVLCDTNGGRLPAEVGRITAEVIAaGVPGDRLGIHTHNDTGNAVANALAAIDAGARQVQGTL 233 **********************************998651689999******************************* PP TIGR00977 232 nGlGercGnanlcslipnlqlklgldv.....iekenlkkltevarlvaeivnlaldenmpyvGesafahkGGvhvs 303 nGlGercGnanl +lip l lk + + l+ l+ v+r + +i n+ +++ +pyvG safahk G+h+s lcl|FitnessBrowser__Dino:3608293 234 NGLGERCGNANLVTLIPTLLLKEPYASrfetgVSAAALRDLVRVSRTLDDILNRVPTKSAPYVGASAFAHKAGLHAS 310 **********************987542222478899**************************************** PP TIGR00977 304 avkrnpktyehidpelvGnkrkivvselaGksnvleklkelGieidekspkvrkilkkikelekqGyhfeaaeasle 380 a+ ++p tyehi pe vGn r i +s++aG+sn+ ++l + Gi++++ + ++ +l+++k e+ Gy f++a+as+e lcl|FitnessBrowser__Dino:3608293 311 AILKDPSTYEHIPPEAVGNARVIPMSNQAGQSNLRKRLADAGIAVQKGDMRLGALLDEVKRREDMGYSFDSAQASFE 387 ***************************************************************************** PP TIGR00977 381 llvrdalGkrkkyfevdgfrvliakrrdee....slseaeatvrvsvegae.....eltaaeGnGpvsaldralrka 448 ll r++l ++fev+ +rv++++r+++ slsea + +++ +e + + ++ Gpv+al +al k lcl|FitnessBrowser__Dino:3608293 388 LLARRHLEGLPEFFEVKRYRVTVERRKNKYdrmvSLSEAVVVLKIGDEKMLsvsesMDETGCDRGPVNALAKALGKD 464 ***********************99887655678999999999999997654444455666779************* PP TIGR00977 449 lekfypslkdlkltdykvrilnesaGtsaktrvliessdGk.rrwgtvGvseniieasytallesieyklrkd 520 l + p ++dl+l+d+kvri n+ Gt+a trvli+s dG+ rrw+tvGvs ni++as+ al+e+i++kl +d lcl|FitnessBrowser__Dino:3608293 465 LGPYTPYIADLRLVDFKVRITNG--GTEAVTRVLIDSEDGQgRRWSTVGVSANIVDASFEALVEAIQWKLIRD 535 ********************986..9**************779***************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (526 nodes) Target sequences: 1 (545 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.02s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 10.51 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory