Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (characterized)
to candidate 3606698 Dshi_0129 dihydroxy-acid dehydratase (RefSeq)
Query= SwissProt::P9WKJ5 (575 letters) >FitnessBrowser__Dino:3606698 Length = 577 Score = 538 bits (1385), Expect = e-157 Identities = 283/556 (50%), Positives = 369/556 (66%), Gaps = 9/556 (1%) Query: 21 SRDVTDGLEKAAARGMLRAVGMDDEDFAKPQIGVASSWNEITPCNLSLDRLANAVKEGVF 80 SR VT+G +A R A+GM +E+ +P +GVA+ WNE PCN++L R A AVK GV Sbjct: 13 SRHVTEGPSRAPHRSYYYAMGMTEEEIHQPLVGVATCWNEAAPCNIALSRQAQAVKMGVK 72 Query: 81 SAGGYPLEFGTISVSDGISMGHEGMHFSLVSREVIADSVEVVMQAERLDGSVLLAGCDKS 140 A G P EF TI+V+DGI+MGHEGM SL SRE IAD+VE+ M+ D V LAGCDKS Sbjct: 73 QASGTPREFTTITVTDGIAMGHEGMRSSLASREAIADTVELTMRGHCYDAIVGLAGCDKS 132 Query: 141 LPGMLMAAARLDLAAVFLYAGSILPGRAKLSDGSERDVTIIDAFEAVGACSRGLMSRADV 200 LPGM+MA RL++ +VF+Y GSILPGR + DVT+ D FEAVG G + A++ Sbjct: 133 LPGMMMAMVRLNVPSVFIYGGSILPGRL-----NGEDVTVQDVFEAVGKHQAGNYTDAEL 187 Query: 201 DAIERAICPGEGACGGMYTANTMASAAEALGMSLPGSAAPPATDRRRDGFARRSGQAVVE 260 + +ER CP GACGG +TANTMA +EA+G++LP SA PA RD + SG+AV++ Sbjct: 188 EVLERVACPSAGACGGQFTANTMACVSEAIGLALPNSAGAPAPYESRDQYGEASGRAVMD 247 Query: 261 LLRRGITARDILTKEAFENAIAVVMAFGGSTNAVLHLLAIAHEANVALSLQDFSRIGSGV 320 L+ +GI ARDI+T+++ ENA +V GGSTNA LHL AIAHEA + +LQD I Sbjct: 248 LIEKGIRARDIVTRKSLENAARIVACTGGSTNAGLHLPAIAHEAGIEFTLQDVCDIFRDT 307 Query: 321 PHLADVKPFGRHVMSDVDHIGGVPVVMKALLDAGLLHGDCLTVTGHTMAENLAAITPPDP 380 P+ D+KP G++V D+ GGVPVVM+ L AGL+H DC+TVTG+++ E L +T + Sbjct: 308 PYFVDLKPGGKYVAKDMYEAGGVPVVMRELRRAGLIHEDCMTVTGYSIGEELDKVT-LEA 366 Query: 381 DGKVLRALANPIHPSGGITILHGSLAPEGAVVKTAGFDSD--VFEGTARVFDGERAALDA 438 DG+V+ + P+ +GG+ L G+LAPEGA+VK AG D VF G ARVF+ E A +A Sbjct: 367 DGRVIYPVDTPLSTTGGVVGLEGNLAPEGAIVKIAGMSDDQLVFTGPARVFECEEDAFEA 426 Query: 439 LEDGTITVGDAVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLLTDGRFSGGTTGLC 498 +++ GD VIR EGP GGPGMREMLA T A+ G G+GK V L+TDGRFSG T G C Sbjct: 427 VQNRAYAEGDVFVIRNEGPAGGPGMREMLATTAALSGQGMGKKVALITDGRFSGATRGFC 486 Query: 499 VGHIAPEAVDGGPIALLRNGDRIRLDVAGRVLDVLADPAEFASRQQDFSPPPPR-YTTGV 557 VGH+ PEA GGPIA+L++GD I +D L V E A+R+ +S P Y +G Sbjct: 487 VGHVGPEAAHGGPIAMLKDGDMITIDALKGELSVALSEDELAARKDAWSGPRETIYASGA 546 Query: 558 LSKYVKLVSSAAVGAV 573 L KY +LV A +GAV Sbjct: 547 LWKYAQLVGGARLGAV 562 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 956 Number of extensions: 48 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 575 Length of database: 577 Length adjustment: 36 Effective length of query: 539 Effective length of database: 541 Effective search space: 291599 Effective search space used: 291599 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate 3606698 Dshi_0129 (dihydroxy-acid dehydratase (RefSeq))
to HMM TIGR00110 (ilvD: dihydroxy-acid dehydratase (EC 4.2.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00110.hmm # target sequence database: /tmp/gapView.24388.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00110 [M=543] Accession: TIGR00110 Description: ilvD: dihydroxy-acid dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6e-208 677.7 3.8 6.9e-208 677.5 3.8 1.0 1 lcl|FitnessBrowser__Dino:3606698 Dshi_0129 dihydroxy-acid dehydra Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3606698 Dshi_0129 dihydroxy-acid dehydratase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 677.5 3.8 6.9e-208 6.9e-208 1 541 [. 24 563 .. 24 565 .. 0.99 Alignments for each domain: == domain 1 score: 677.5 bits; conditional E-value: 6.9e-208 TIGR00110 1 aarallkatGlkdedlekPiiavvnsyteivPghvhlkdlaklvkeeieaaGgvakefntiavsDGiamgheGmkys 77 ++r+ ++a+G+++e++++P+++v+ +++e +P+++ l+ a++vk ++++a g++ ef ti+v+DGiamgheGm+ s lcl|FitnessBrowser__Dino:3606698 24 PHRSYYYAMGMTEEEIHQPLVGVATCWNEAAPCNIALSRQAQAVKMGVKQASGTPREFTTITVTDGIAMGHEGMRSS 100 689************************************************************************** PP TIGR00110 78 LpsreiiaDsvetvvkahalDalvvissCDkivPGmlmaalrlniPaivvsGGpmeagktklsekidlvdvfeavge 154 L+sre iaD+ve +++h++Da+v ++ CDk +PGm+ma++rln+P+++++GG++ +g+++ +e++++ dvfeavg+ lcl|FitnessBrowser__Dino:3606698 101 LASREAIADTVELTMRGHCYDAIVGLAGCDKSLPGMMMAMVRLNVPSVFIYGGSILPGRLN-GEDVTVQDVFEAVGK 176 *************************************************************.9************** PP TIGR00110 155 yaagklseeeleeiersacPtagsCsGlftansmacltealGlslPgsstllatsaekkelakksgkrivelvkkni 231 +ag+ +++ele +er+acP+ag+C+G ftan+mac++ea+Gl+lP+s+ ++a + + + + sg+ +++l++k i lcl|FitnessBrowser__Dino:3606698 177 HQAGNYTDAELEVLERVACPSAGACGGQFTANTMACVSEAIGLALPNSAGAPAPYESRDQYGEASGRAVMDLIEKGI 253 ***************************************************************************** PP TIGR00110 232 kPrdiltkeafenaitldlalGGstntvLhllaiakeagvklslddfdrlsrkvPllaklkPsgkkviedlhraGGv 308 + rdi+t++++ena ++ +GGstn+ Lhl+aia+eag++++l+d+ + r +P ++lkP+gk+v +d+ +aGGv lcl|FitnessBrowser__Dino:3606698 254 RARDIVTRKSLENAARIVACTGGSTNAGLHLPAIAHEAGIEFTLQDVCDIFRDTPYFVDLKPGGKYVAKDMYEAGGV 330 ***************************************************************************** PP TIGR00110 309 savlkeldkegllhkdaltvtGktlaetlekvkvlrvdqdvirsldnpvkkegglavLkGnlaeeGavvkiagveed 385 + v++el ++gl+h+d++tvtG +++e+l+kv++ +d vi ++d p++ +gg+ L Gnla+eGa+vkiag+++d lcl|FitnessBrowser__Dino:3606698 331 PVVMRELRRAGLIHEDCMTVTGYSIGEELDKVTLE-ADGRVIYPVDTPLSTTGGVVGLEGNLAPEGAIVKIAGMSDD 406 *********************************99.8999************************************* PP TIGR00110 386 ilkfeGpakvfeseeealeailggkvkeGdvvviryeGPkGgPGmremLaPtsalvglGLgkkvaLitDGrfsGgtr 462 l+f+Gpa+vfe ee+a ea+ ++ eGdv vir eGP GgPGmremLa t+al+g G+gkkvaLitDGrfsG+tr lcl|FitnessBrowser__Dino:3606698 407 QLVFTGPARVFECEEDAFEAVQNRAYAEGDVFVIRNEGPAGGPGMREMLATTAALSGQGMGKKVALITDGRFSGATR 483 ***************************************************************************** PP TIGR00110 463 GlsiGhvsPeaaegGaialvedGDkikiDienrkldlevseeelaerrakakkkea.revkgaLakyaklvssadkG 538 G+++Ghv Peaa+gG+ia+++dGD+i+iD+ + +l +++se+ela+r+++++ +++ + +gaL kya+lv +a G lcl|FitnessBrowser__Dino:3606698 484 GFCVGHVGPEAAHGGPIAMLKDGDMITIDALKGELSVALSEDELAARKDAWSGPREtIYASGALWKYAQLVGGARLG 560 ***************************************************977653799***************** PP TIGR00110 539 avl 541 av+ lcl|FitnessBrowser__Dino:3606698 561 AVT 563 *97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (543 nodes) Target sequences: 1 (577 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 11.69 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory