Aligments for a candidate for ilvE in Dinoroseobacter shibae DFL-12

GapMind for Amino acid biosynthesis

Aligments for a candidate for ilvE in Dinoroseobacter shibae DFL-12

Align Putative branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42; Transaminase B (uncharacterized)
to candidate 3609919 Dshi_3301 branched-chain amino acid aminotransferase (RefSeq)

Query= curated2:O27481
         (306 letters)



>lcl|FitnessBrowser__Dino:3609919 Dshi_3301 branched-chain amino
           acid aminotransferase (RefSeq)
          Length = 289

 Score =  214 bits (545), Expect = 2e-60
 Identities = 116/294 (39%), Positives = 175/294 (59%), Gaps = 14/294 (4%)

Query: 4   EASGKIWLNGEMVEWEEATVHVLSHVVHYGSSVFEGIRCYRNSKGSAIFRLREHVKRLFD 63
           +  G IW++G++V+W  A VH+L+H +HY SSVFEG R Y       IF  R H +RL  
Sbjct: 7   DRDGTIWMDGKLVDWRAANVHILTHAMHYASSVFEGERAYNGK----IFESRRHSERLHF 62

Query: 64  SAKIYRMDIPYTQEQICDAIVETVRENGLEECYIRPVVFRGYG-EMGVHPVNCPVDVAVA 122
           SA    ++IP+T ++I  A  E ++ NGL + Y+R V +RG G +MGV     PV +A+A
Sbjct: 63  SAGELDIEIPFTVDEIEAAKYEVMKVNGLSDAYVRAVAWRGAGPDMGVASARNPVHLAIA 122

Query: 123 AWEWGAYLGAEALEVGVDAGVSTWRRMAPNTMPNMAKAGGNYLNSQLAKMEAVRHGYDEA 182
            WEWG+Y G +A   G    ++ W+R +P T+P  AKA G Y+    +K  A   G  +A
Sbjct: 123 CWEWGSYYG-DAKTRGAKLDIAKWKRPSPETIPVHAKAAGLYMICTTSKHAAEAKGCSDA 181

Query: 183 IMLDYHGYISEGSGENIFLVSEGEIYTPPVSSSLLRGITRDSVIKIARTEGVTVHEEPIT 242
           + +DY GY++E +G NIF V +GE++TP      L GITR +VI + R + V VHE  I 
Sbjct: 182 LFMDYRGYVAEATGANIFFVKDGEVHTPD-PDCFLNGITRQTVIGMLRDKQVKVHERHIM 240

Query: 243 REMLYIADEAFFTGTAAEITPIRSVD--GIEIGAGRRGPVTKLLQDEFFRIIRA 294
            E L   ++ + TGTAAE+TP+  +     E+GA     +T+ + + + +++RA
Sbjct: 241 PEELEGFEQCWLTGTAAEVTPVGQIGTYNFEVGA-----LTRDIAESYEQLVRA 289


Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 286
Number of extensions: 16
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 306
Length of database: 289
Length adjustment: 26
Effective length of query: 280
Effective length of database: 263
Effective search space:    73640
Effective search space used:    73640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate 3609919 Dshi_3301 (branched-chain amino acid aminotransferase (RefSeq))
to HMM TIGR01122 (ilvE: branched-chain amino acid aminotransferase (EC 2.6.1.42))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01122.hmm
# target sequence database:        /tmp/gapView.5692.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01122  [M=298]
Accession:   TIGR01122
Description: ilvE_I: branched-chain amino acid aminotransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
      7e-84  267.5   0.0    7.9e-84  267.3   0.0    1.0  1  lcl|FitnessBrowser__Dino:3609919  Dshi_3301 branched-chain amino a


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Dino:3609919  Dshi_3301 branched-chain amino acid aminotransferase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  267.3   0.0   7.9e-84   7.9e-84       1     285 [.      13     288 ..      13     289 .] 0.98

  Alignments for each domain:
  == domain 1  score: 267.3 bits;  conditional E-value: 7.9e-84
                         TIGR01122   1 wldGelvdvedakvhvlthalhYGtgvfeGiRaYetdkglaifrlkehveRlydsakilrleipyskeelvevtkev 77 
                                       w+dG+lvd++ a+vh+ltha+hY ++vfeG RaY++     if  + h eRl+ sa  l++eip++ +e+  +  ev
  lcl|FitnessBrowser__Dino:3609919  13 WMDGKLVDWRAANVHILTHAMHYASSVFEGERAYNG----KIFESRRHSERLHFSAGELDIEIPFTVDEIEAAKYEV 85 
                                       9***********************************....9************************************ PP

                         TIGR01122  78 lrknnlksaYiRplvyvGae.dlglkpkvdlkveviiaawewgaylgeealekGikvkvssfrraavnsiptkakaa 153
                                       +++n+l++aY+R ++++Ga+ d+g+   +  +v+++ia wewg y+g+ a  +G k  ++ ++r ++++ip +akaa
  lcl|FitnessBrowser__Dino:3609919  86 MKVNGLSDAYVRAVAWRGAGpDMGVAS-ARNPVHLAIACWEWGSYYGD-AKTRGAKLDIAKWKRPSPETIPVHAKAA 160
                                       ******************9769*****.888****************6.89************************** PP

                         TIGR01122 154 gnYlnsllaksealraGydeailLdeeGyvaeGsGenifivkdgvlltPpvsesiLkgitrdaviklakelgievke 230
                                       g Y+  +  k+ a ++G  +a+++d  Gyvae +G nif vkdg++ tP+  +  L+gitr++vi +++++ ++v+e
  lcl|FitnessBrowser__Dino:3609919 161 GLYMICTTSKHAAEAKGCSDALFMDYRGYVAEATGANIFFVKDGEVHTPDP-DCFLNGITRQTVIGMLRDKQVKVHE 236
                                       ***************************************************.99*********************** PP

                         TIGR01122 231 erisreelytaDevfltGtaaevtPirevDgrkigegkrGpvtkklqeaffdlve 285
                                       ++i  eel   +  +ltGtaaevtP+ ++      + ++G +t+ + e++ +lv+
  lcl|FitnessBrowser__Dino:3609919 237 RHIMPEELEGFEQCWLTGTAAEVTPVGQIGTY---NFEVGALTRDIAESYEQLVR 288
                                       ***************************99876...568999**********9987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (298 nodes)
Target sequences:                          1  (289 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 9.02
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis